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111.
Ju Kyoung Oh Jong Pyo Chae Edward Alain B. Pajarillo Sung Hun Kim Min‐Jin Kwak Jong‐Su Eun Seok Woo Chee Kwang‐Youn Whang Sae‐Hun Kim Dae‐Kyung Kang 《Animal Science Journal》2020,91(1)
Gastrointestinal microbiota impact host's biological activities, including digestion of indigestible feed components, energy harvest, and immunity. In this study, fecal microbiota of high body weight (HW) and low body weight (LW) growing pigs at 103 days of age were compared. Principal coordinates analysis separated the HW and LW groups into two clusters, indicating their potential differences between microbial community composition. Although the abundances of two major phyla, Firmicutes and Bacteroidetes, did not significantly differ between the HW and LW groups, some genera showed significant differences. Among them, Peptococcus and Eubacterium exhibited strong positive correlations with body weight (BW) and average daily gain (ADG) (Rho > 0.40), whereas Treponema, Desulfovibrio, Parabacteroides, and Ruminococcaceae_unclassified exhibited strong negative correlations with BW and ADG (Rho < ?0.40). Based on these results, the structure of intestinal microbiota may affect growth traits in pigs through host–microbe interactions. Further in‐depth studies will provide insights into how best to reshape host–microbe interactions in pigs and other animals as well. 相似文献
112.
Dominique Hazard Tiphaine Macé Amandine Kempeneers Eric Delval Didier Foulquié Jacques Bouix Alain Boissy 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(4):374-383
In livestock, improving maternal reactivity towards the litter is an important issue in breeding strategies to promote production and animal welfare. As of yet, no studies have investigated the within-breed genetic variation of maternal reactivity in sheep. The objective of this study was to estimate the genetic parameters of maternal reactivity traits. A total of 1,095 primiparous and 1,441 multiparous Romane ewes were phenotyped 24 hr postlambing using a behavioural test (arena test, AT) over a 10-year experimental period. The test consisted of three successive phases evaluating the ewe's attraction to her litter, reactivity to separation from her litter, and reactivity to a conflict between attraction to her litter and avoidance of a motionless human. The ewes were reared exclusively on rangelands (South of France) and lambed outdoors in the spring. High-pitched bleating and low-pitched bleating in the AT were mostly highly heritable (0.39‒0.46). Heritabilities were moderate for proximity to the litter in the presence of a human (0.27) and low for locomotion and vigilance in the AT (0.09‒0.15). The measurements of a given behaviour in the three phases of the AT were highly genetically correlated. Few genetic correlations were found between the different behavioural traits in the AT, the highest correlations being between high-pitched bleating and low-pitched bleating (−0.43 to −0.77). In conclusion, our findings demonstrate moderate-to-high heritability for maternal reactivity traits. These traits could be included in genetic selection schemes to enhance maternal attachment provided there is no unfavourable link with other production traits. 相似文献
113.
This greenhouse study aimed to analyze the impact of arbuscular mycorrhizal (AM) fungal associations on maize (Zea mays L. hybrid Pioneer 3905) in order to compare their functional compatibility and efficiency. The AM fungus species used for this study were Glomus aggregatum, G. etunicatum, G. mosseae, and G. versiforme. Shoot and leaf masses, chlorophyll, soluble protein, total and reducing sugar, carbon (C), and nitrogen (N) concentrations, and glutamine synthetase (GS) activity in the maize leaves were analyzed. The root colonization ranged from 26% to 72% depending on the AM fungus species. Leaf mass was significantly higher when maize plants were colonized with G. etunicatum in comparison to the non‐AM control. The mycorrhizal effect on dry leaf mass ranged from 15.9% to 23.9% depending on the AM species. However, the total shoot mass did not differ significantly among the treatments. The mycorrhizal treatment had a marginally significant effect on the chlorophyll concentrations in maize leaves. The protein concentration was the highest in the plants colonized with G. etunicatum and the N percentage was significantly higher in the leaves of plants colonized by G. versiforme or G. aggregatum than those with G. mosseae. However, the AM colonization did not significantly alter the GS activity among the treatments. The highest sugar concentrations were detected in the leaves of plants colonized by G. versiforme. The sugar concentrations as well as the C percentage were lower in the leaves of plants colonized by G. etunicatum compared to the other mycorrhizal treatments but the values were comparable to the non‐AM control. Our overall results suggest that the expression of the mycorrhizal potential in the maize host plants varies among AM fungal species. 相似文献
114.
115.
Background
About 4 × 105 eggs in more than 5000 marine turtle nests are deposited every year on a 3.6 km long beach in French Guiana (South America). The dry biomass of eggs is estimated to be 5 × 103 kg, yet only 25% of this organic matter will return to the ocean in the form of hatchlings. Such amounts of organic matter are supposed to drive the functioning of the beach ecosystem. Previous studies have shown that egg predators and detritivorous organisms dominate the trophic relationships and the dynamics of the system. The role of a terrestrial insect Scapteriscus didactylus (Latreille), which damages up to 40% of the eggs of the marine turtle (Dermochelys coriacea), was unexpected. However it was impossible from direct observations to prove that the mole cricket consumed a significant amount of these eggs. Therefore, the precise place of the mole cricket in the nitrogen and carbon cycles of the beach ecosystem could not be determined. In order to answer this question, we looked for a marine signature of carbon and nitrogen source metabolized by the mole cricket. 相似文献116.
Guylaine Blanc Alain Charcosset Jean-Baptiste Veyrieras André Gallais Laurence Moreau 《Euphytica》2008,161(1-2):71-84
Many studies already investigated marker-assisted selection (MAS) efficiency but mainly in biparental populations. Connected
multiparental populations address a broader diversity and confer a gain of power for QTL detection which must be beneficial
for MAS. Our objective was to compare multiparental connected designs to biparental populations taken separately for MAS and
phenotypic selection. We first detected QTL for flowering time and grain yield in an experimental maize design involving four
parental inbred lines crossed to produce six different biparental populations and confirmed the advantage of multiparental
connected designs over biparental populations for QTL detection. Based on these results we performed stochastic simulations
to evaluate the expected efficiency of four generations of MAS and phenotypic selection. Different parameters were considered:
trait heritability, genetic architecture and whether QTL were assumed to be known or have to be detected. Genetic gains were
higher in the multiparental design than on average over the biparental populations considered separately, especially when
favourable alleles were equally distributed among parental lines. When QTL detection was included in the simulation process,
we found that type I error risk considered for declaring QTL as significant should be adapted to the design. Type I error
risks leading to the best response were higher for the biparental populations than for the multiparental design. Besides addressing
a broader diversity, multiparental designs increase the power of QTL detection, which reinforces their superiority over biparental
designs for MAS. Application of MAS to multiparental designs therefore appears promising to accelerate genetic gain in plant
breeding programs. 相似文献
117.
Taissir Abou Al Fadil Alain Jauneau Yves Martinez Martina Rickauer Grégory Dechamp-Guillaume 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):93-103
Phoma macdonaldii, the causal agent of black stem disease of sunflower (Helianthus annuus), also attacks roots and collars of the plants, resulting in early death. Totally resistant lines do not exist for infection
of the aerial parts, but tolerant lines have been characterised. This paper presents a study on colonisation of a partially
resistant and a susceptible sunflower line by P. macdonaldii. The fungus was transformed with a constitutively expressed reporter gene encoding the jellyfish green fluorescent protein
via Agrobacterium tumefaciens, and colonisation of sunflower roots by this transformed strain was studied by various microscopy techniques including confocal
and scanning electron microscopy. The results show that penetration of the fungus into the root occurred through natural fissures
or through the epidermis and was similar in both lines. In contrast, the colonisation rate of the stele was reduced in the
partially resistant line, and the morphology of the fungal hyphae was also affected. The effect on hyphal morphology was strongest
in the stele, indicating a localised production of defence compounds in this line. 相似文献
118.
Pierre-Louis Lucas Rodolphe Dumontier Corinne Loutelier-Bourhis Alain Mareck Carlos Afonso Patrice Lerouge Narimane Mati-Baouche Muriel Bardor 《Plant methods》2018,14(1):107
Background
Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor. This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins. The N-linked glycans bore by the glycoproteins are then processed into oligomannosides prior to the exit of the glycoproteins from the endoplasmic reticulum and its entrance into the Golgi apparatus. In this compartment, the N-linked glycans are further maturated in complex type N-glycans. This process has been well studied in a lot of eukaryotes including higher plants. In contrast, little information regarding the LLO precursor and synthesis of N-linked glycans is available in microalgae.Methods
In this report, a user-friendly extraction method combining microsomal enrichment and solvent extractions followed by purification steps is described. This strategy is aiming to extract LLO precursor from microalgae. Then, the oligosaccharide moiety released from the extracted LLO were analyzed by multistage tandem mass spectrometry in two models of microalgae namely the green microalgae, Chlamydomonas reinhardtii and the diatom, Phaeodactylum tricornutum.Results
The validity of the developed method was confirmed by the analysis of the oligosaccharide structures released from the LLO of two xylosyltransferase mutants of C. reinhardtii confirming that this green microalga synthesizes a linear Glc3Man5GlcNAc2 identical to the one of the wild-type cells. In contrast, the analysis of the oligosaccharide released from the LLO of the diatom P. tricornutum demonstrated for the first time a Glc2Man9GlcNAc2 structure.Conclusion
The method described in this article allows the fast, non-radioactive and reliable multistage tandem mass spectrometry characterization of oligosaccharides released from LLO of microalgae including the ones belonging to the Phaeodactylaceae and Chlorophyceae classes, respectively. The method is fully adaptable for extracting and characterizing the LLO oligosaccharide moiety from microalgae belonging to other phyla.119.
Alain Regnier Clara Landrevie Marie-Claire Robin Delphine Einsweiler Jean-Yves Douet Isabelle Raymond-Letron 《Veterinary ophthalmology》2023,26(1):62-70
A 6-year-old miniature Shetland pony mare was referred for evaluation of a left corneal mass, which developed from the healing tissue of a corneal traumatic ulceration that had occurred 4 weeks previously. On gross examination, a spherical, smooth-surfaced, and pink-colored lesion of about 1 cm in diameter was protruding from the left palpebral fissure. Ophthalmic examination revealed that it was attached to the scar tissue of the cornea, and that one corpora nigra was adherent to the posterior face of corneal wounded area, without sign of uveitis. The remainder of the ophthalmic examination was unremarkable. The mass was excised, and cryotherapy was used as an adjunctive therapy. Histopathology of the resected mass was consistent with a pyogenic granuloma on the basis of radially oriented proliferating capillaries, embedded in immature granulation tissue containing an infiltrate of neutrophils, plasma cells and eosinophils. There were no histological features of malignancy. 2 months after surgery, the ventral part of the fibrotic corneal scar was slightly raised by a pink tissue, suggesting possible recurrence of the initial lesion. A second cryotherapy was performed over the leukoma area. No recurrence has been noted for a follow-up period of more than 25 months. Pyogenic granuloma is a benign proliferative fibrovascular response that typically develops after trauma or surgery. Corneal involvement is rare in humans, and to the authors' knowledge has never been documented in veterinary ophthalmology. 相似文献
120.
Roger R.B. Leakey Zacharie Tchoundjeu Rognvald I. Smith Robert C. Munro Jean-Marie Fondoun Joseph Kengue Paul O. Anegbeh Alain R. Atangana Annabelle N. Waruhiu Ebenezer Asaah Cecilia Usoro Victoria Ukafor 《Agroforestry Systems》2004,60(2):101-111
Ten fruit and kernel traits were measured in 152 Irvingia gabonensis and 293 Dacryodes edulis trees from 6 villages in Cameroon and Nigeria. Frequency distribution curves were used to examine the range of variation
of each trait of each species in each village and aggregated into national and regional populations. There were differences
between the village sub-populations, with regard to the normality (e.g., mean kernel mass of D. edulis) or skewness (e.g., mean flesh depth of D. edulis) of the distribution curves and in the degree of separation between the individual village populations along the x axis,
resulting in the development of a bimodal distribution in the regional population. For all traits, populations of both species
differed significantly between countries, but only in D. edulis were there significant differences between the Cameroon populations. On the basis of the results of this study, D. edulis can be said to be virtually wild in Nigeria but semi-domesticated in Cameroon, while I. gabonensis is wild in Cameroon and semi-domesticated in Nigeria. These results are discussed with regard to a hypothesis that the range
and frequency of variation in the different populations can be used to identify five stages of domestication. From a comparison
of the frequency distribution curves of desirable versus undesirable traits, and statistically identifyable changes in skewness and kurtosis, it is concluded that as a result of
the farmers’ own efforts by truncated selection, D. edulis is between Stages 2 and 3 of domestication (with a 67% relative gain in flesh depth) in Cameroon, while I. gabonensis in Nigeria is at Stage 2 (with a 44% relative gain in flesh depth). In this study, genetic diversity seems to have been increased,
and not reduced, by domestication.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献