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101.
102.
Tariq Mahmood Aysha Jameel Bilal Haider Abbasi Faiza Munir Syed Muhammad Saqlan Naqvi 《Journal of Crop Science and Biotechnology》2012,15(4):289-295
Planago ovata L. is an economically important species in the monotypic genus Plantago. It is a short-stemmed annual herb. The seed husk of this plant is commonly called psyllium or isabgol which is important in pharmaceutical formulation and food industry. In this study, callus induction was optimized using different explants of Plantago ovata. Callus DNA was utilized to access the somaclonal variations using the Random Amplification of Polymorphic DNA (RAPD) markers. The maximum callus growth was observed in Murashige and Skoog (MS) medium containing 4 mg L?1 2,4-D concentration for shoots, 0.5 mg L?1 for seeds and 2 mg L?1 for roots. Moreover, the effect of culture age was considered in assessing genetic variability. Maximum genetic variability was observed in the DNA samples of callus at the concentration of 2 mg L?1 2,4-D for all explants (roots, shoots, and seeds). Cluster analysis was performed based on 1) similarity coefficient between samples and 2) molecular data using the Numerical Taxonomy and Multivariate Analysis System (NTSYS) PC version 2.01; similarity index was generated by similarity for Quantitative Data (SIMQUAL). Our study indicated that Random Amplified Polymorphic DNAs can successfully be used to explore polymorphism among callus samples at different hormonal concentrations. This study can be useful for the production of callus from Plantago ovata and estimation of genetic variations due to tissue culture conditions. Evaluation of genetic variations can display novel features and manipulate genetic bottlenecks in Plantago ovata. New genetic variations in somaclones can bring vital insight for plant improvement. 相似文献
103.
Waseem Shahzad Rashid Munir Mohammad S. Khan Mansur D. Ahmad Mohammad Ijaz Ashfaq Ahmad Mohammad Iqbal 《Tropical animal health and production》2010,42(8):1597-1599
The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies. 相似文献
104.
In vivo quantification of muscle damage in dogs after general anaesthesia with halothane andpropofol
B. M. Aktas P. Vinclair A. Autefage H. P. Lefebvre P. L. Toutain J. P. Braun 《The Journal of small animal practice》1997,38(12):565-569
Muscle damage in dogs anaesthetised with halothane and propofol was quantified by measurement of the area under the curve of plasma creatine kinase (CK) versus time. Plasma CK remained unchanged during anaesthesia for two and a half and five hours. Following halothane anaesthesia of dogs (resting on one side directly on the surgical table or on cushions, and with or without rotation of the body every 30 minutes), plasma CK was elevated in some animals to 10 000 U/litre by the 12th hour (baseline value ≤100 u/litre), whereas it remained almost unchanged in other animals. Plasma CK then returned to reference values on day 2 or 3. The mean equivalent of muscle damaged ranged from 0–6 to 0–9 g/kg bodyweight. No muscle damage could be demonstrated in animals anaesthetised with propofol. It is therefore concluded that plasma CK should not be used as a diagnostic aid following halothane anaesthesia because of false positives due to the halothane anaesthesia itself and that propofol Is best suited for the investigation of muscle damage due to surgical procedures. 相似文献
105.
Chemical interaction of Earth's mantle with the liquid outer core should influence the mantle's iron content. Osmium isotope ratios in Hawaiian lavas indicate a mass flux of =1% core to the mantle, which is the immediate source of these lavas. We present precise measurements of the Fe/Mn ratio for Hawaiian lavas, revealing an increase of 1 to 2% in the mole fraction of iron in the mantle beneath Hawaii. This corresponds to a density anomaly of about 0.5%, about the same magnitude observed in seismic tomography models of the Pacific superswell region. These data also rule out a role for Mn-rich sediments as the source of the Hawaiian Os isotope signal. 相似文献
106.
107.
Detection and identification of Theileria and Babesia species in 920 apparently healthy small ruminants in eastern Turkey, as well as parasite genetic diversity, was investigated using a specifically designed reverse line blot (RLB) assay. The hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene was amplified and hybridized to a membrane onto which catchall and species-specific oligonucleotide probes were covalently linked. Three Theileria and one Babesia genotype were identified. Comparison of the Theileria genotypes revealed 93.6-96.2% similarity among their 18S rRNA genes. Two Theileria shared 100% and 99.7% similarity with the previously described sequences of T. ovis and Theileria sp. OT3, respectively. A third Theileria genotype was found to be clearly different from previously described Theileria species. The genotype was provisionally designated as Theileria sp. MK. The Babesia genotype shared 100% similarity with Babesia ovis. The survey indicated a high prevalence of piroplasm infections in small ruminants (38.36%). Theileria spp. prevalence was 36.08%. Prevalence of B. ovis was 5.43%. The most abundant Theileria species identified was T. ovis (34.56%) followed by Theileia sp. MK (1.30%) and Theileria sp. OT3 (0.43%). 相似文献
108.
Aqil Tariq Hong Shu Saima Siddiqui Iqra Munir Alireza Sharifi Qingting Li Linlin Lu 《林业研究》2022,33(1):183-194
Most forest fires in the Margalla Hills are related to human activities and socioeconomic factors are essential to assess their likelihood of occurrence.This study consid-ers both environmental (altitude,precipitation,forest type,terrain and humidity index) and socioeconomic (popula-tion density,distance from roads and urban areas) factors to analyze how human behavior affects the risk of forest fires.Maximum entropy (Maxent) modelling and random forest (RF) machine learning methods were used to predict the probability and spatial diffusion patterns of forest fires in the Margalla Hills.The receiver operating characteristic(ROC) curve and the area under the ROC curve (AUC) were used to compare the models.We studied the fire history from 1990 to 2019 to establish the relationship between the prob-ability of forest fire and environmental and socioeconomic changes.Using Maxent,the AUC fire probability values for the 1999s,2009s,and 2019s were 0.532,0.569,and 0.518,respectively;using RF,they were 0.782,0.825,and 0.789,respectively.Fires were mainly distributed in urban areas and their probability of occurrence was related to acces-sibility and human behaviour/activity.AUC principles for validation were greater in the random forest models than in the Maxent models.Our results can be used to establish preventive measures to reduce risks of forest fires by consid-ering socio-economic and environmental conditions. 相似文献
109.
Tariq-Khan Muhammad Munir Anjum Mukhtar Tariq Hallmann Johannes Heuer Holger 《植物病害和植物保护杂志》2017,124(3):201-212
Journal of Plant Diseases and Protection - Root-knot nematodes (RKN) are yield devastating plant pathogens. Objective of this study was to investigate the distribution of RKN and their threat to... 相似文献
110.
Kursat Ozer Aysun Yilmaz Mariano Carossino Gulay Yuzbasioglu Ozturk Ozge Erdogan Bamac Hasan E. Tali Egemen Mahzunlar Utku Y. Cizmecigil Ozge Aydin Hamid B. Tali Semaha G. Yilmaz Zihni Mutlu Ayse Ilgn Kekec Nuri Turan Aydin Gurel Udeni Balasuriya Munir Iqbal Juergen A. Richt Huseyin Yilmaz 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(4)
This paper reports a presumptive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in a cat. A cat with respiratory disease living with three individuals with coronavirus disease 2019 showed bilateral ground-glass opacities in the lung on X-ray and computed tomography. The clinical swabs were negative for SARS-CoV-2 RNA, but the serum was positive for SARS-CoV-2 antibodies. Interstitial pneumonia and prominent type 2 pneumocyte hyperplasia were noted on histopathology. Respiratory tissues were negative for SARS-CoV-2 RNA or antigen, but the cat was positive for feline parvovirus DNA. In conclusion, the respiratory disease and associated pathology in this cat could have been due to exposure to SARS-CoV-2. 相似文献