Influence of High Shoot and Root-Zone Temperatures on Growth of Three Wheat Genotypes during Early Vegetative Stages

High temperatures, whether of shoot or root, are reported to affect shoot and root growth of various plant species. The scanty information available on the differential response of wheat genotypes to high shoot and root‐zone temperatures triggered this investigation to study the response and adaptation of shoot and root growth of three wheat genotypes to high shoot and root‐zone temperatures during early growth stages. Three wheat genotypes; Fang (heat tolerant), Siete Cerros (heat sensitive) and Imam (recent cultivar adapted to a hot irrigated environment) were grown in soil and hydroponically. Three shoot/root‐zone temperatures (23/23, 23/35 and 35/35 °C for the soil experiment and 22/22, 22/38 and 38/38 °C for the hydroponic experiment) were applied at three‐leaf growth stage. High root‐zone temperature alone or combined with high shoot temperature reduced xylem sap flow rate, root dry weight, root length and root/shoot ratio. Unexpectedly, shoot fresh and dry weights and relative growth rate (RGR) were not significantly affected by the high root‐zone temperature except for the susceptible genotype, Siete Cerros, after prolonged exposure in the hydroponic experiment. In contrast, high shoot/root‐zone temperature significantly reduced shoot fresh and dry weights from as early as the first week of the hydroponic experiment. The 38/38 °C treatment also caused significant reduction in RGR and net assimilation rate during the first 2 weeks, but no significant differences were found during the last 2 weeks compared with 22/22 °C. Interesting responses were observed among genotypes in terms of shoot and root dry weights and root/shoot ratio at 38/38 °C treatment. The heat‐sensitive Siete Cerros showed the least reduction in these traits during the first 2 weeks while the heat‐tolerant Fang and Imam responded by greatly reducing their shoot and root weights. The situation was almost reversed with the duration of treatments such that Siete Cerros became the most affected genotype while Fang and Imam were better adapted to high shoot/root‐zone temperature. Specific root weight was the exceptional trait that increased under high temperature treatments. Results indicate that despite the reduction in root length and weight observed under high root‐zone temperature, shoot growth was not much affected suggesting that the use of suitable cultivar coupled with proper management could alleviate most of high root‐zone temperature effects during early growth stages.     

Effects of omega-7 palmitoleic acids on skeletal muscle differentiation in a hyperglycemic condition

Maternal obesity and diabetes are known to be involved in fetal myogenesis, but the later stages of myogenesis are not well understood. In this study, we investigated the influence of a hyperglycemic environment on L6 skeletal myoblast differentiation and the function of omega-7 palmitoleic acids. Exposure to a high concentration of glucose (25 mM) in high-glucose culture medium (HG) increased the expression of myogenic genes (MyoD, Myogenin, MRF4, Myhc2x, and Myhc2a) and the synthesis of myosin. HG also activated the PI3K/AKT pathway revealed muscle cell differentiation. Furthermore, the levels of reactive oxygen species (ROS) and an inflammatory cytokine (Tnfaip3; tumor necrosis factor alpha-induced protein 3), which are crucial for the growth and differentiation of skeletal muscle, were increased by HG. Palmitoleic acids suppressed the expression levels of myogenic regulatory genes and increased the expression level of a cell proliferation-related gene (Pax3). Trans-palmitoleic acid and eicosapentaenoic acid (TPA and EPA) increased the phosphorylation level of MAPK/ERK1/2 and downregulated ROS generation and Tnfaip3 expression. In contrast, cis-palmitoleic acid inactivated MAPK/ERK1/2, leading to increased ROS generation. In conclusion, a hyperglycemic environment mediated by HG induced excessive muscle differentiation. Palmitoleic acids inhibited myoblast differentiation by downregulating muscle-specific genes. Moreover, trans-palmitoleic acids may have beneficial antioxidant and/or anti-inflammatory effects in cells.     

Evaluation of three phytoplankton species as food for the pearl oyster <Emphasis Type="Italic">Pinctada fucata</Emphasis>

In the pearl cultivation farms of the Ehime Prefecture, Japan, mass mortalities of the pearl oyster Pinctada fucata have occurred since 1994. The occurrences of mass mortality roughly coincided with a shift of the dominant phytoplankton from Skeletonema and Chaetoceros to Chaetoceros and Nitzschia all of which belong to Bacillariophyceae. Hence, we evaluated Nitzschia, together with Chaetoceros and Isocrysis, as food for the oyster. Wet weights, lengths, widths, glycogen contents, and growth rates in terms of wet weight of the oysters in all the feeding treatments were significantly higher than those in the non-feeding treatment. The highest glycogen content (2.34%) and growth rate (2.21 g month⁻¹) were found in the Chaetoceros treatment. Growth rate in the Isocrysis treatment (1.63 g month⁻¹) was also high, although glycogen content in this treatment (0.41%) was low. In the Nitzschia treatment, growth rate of the oyster (0.94 g month⁻¹) was the lowest and glycogen content (0.83%) was also low relative to that in the Chaetoceros treatment. Chlorophyll a concentration in fecal pellets was lowest in the Nitzschia treatment (<2.7 μg mg⁻¹), suggesting more complete digestion of Nitzschia by the oyster. Thus, Nitzschia was edible and digestible but not assimilated by P. fucata. We propose the following scenario for the relationship between Nitzschia dominance and mass mortality. When Nitzschia dominates in a culture area, the physiological condition of P. fucata deteriorates due to low assimilation of Nitzschia by the oyster, followed by susceptibility of the oyster to infection by agents lethal to the oyster.     

Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus

Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.     

Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage

The aim of the present study was to clarify the overall efficiency of porcine somatic cell nuclear transfer (SCNT) by incorporating cryopreservation of the cloned embryos before transfer. The SCNT embryos reconstructed with preadipocytes and in vitro-matured (IVM) oocytes were cultured to harvest morula stage embryos; they were then subjected to delipation (removal of cytoplasmic lipid droplets) and vitrification. After warming and culture, the embryos developing to blastocysts were transferred to recipients to obtain cloned piglets. From 372 reconstructed embryos, 188 (50.5%) reached the morula stage and 117 (31.5%) developed to blastocysts after vitrification. Transfer of 98 (26.3%) morphologically normal blastocysts gave rise to 6 (1.6%) piglets, including 1 stillborn. The efficiency of the cloned piglet production was comparable with that obtained using SCNT embryos without cryopreservation (2.7%, 17/635). Here, we demonstrate that porcine somatic cell cloning can be performed without a significant reduction in efficiency even when the SCNT embryos are cryopreserved before transfer.     

Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.     

Effect of growth on wood properties for Japanese larch (Larix kaempferi): Differences of annual ring structure between corewood and outerwood

To investigate the relations between growth and the wood properties of Japanese larch (Larix kaempferi), six sample trees of varied ages and radial growth were felled and the ring width, ring density, percentage of latewood, and some other factors were determined. There were significant differences in ring density and percentage of latewood between sample trees with vigorous growth and those with poor growth. In corewood the ring density decreased with increasing ring width for all sample trees, whereas in outerwood this trend did not appear. Moreover, the latewood width increased with the increment of ring width only in outerwood, whereas there was almost no change in the corewood. The variation in patterns of ring width, ring density, and percentage of latewood in the radial direction and the relation with height was also studied.Part of this report was presented at the 47th annual meeting of the Japan Wood Research Society, Kochi, April 1997