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排序方式: 共有176条查询结果,搜索用时 31 毫秒
151.
Muhammad I Illijas Masaru Terasaki Ryo Nakamura Noriaki Iijima Akihiko Hara Nobuhiro Fusetani Yutaka Itabashi 《Fisheries Science》2008,74(3):670-676
ABSTRACT: A glycerolipid acyl-hydrolase was purified 19-fold with a yield of 11% from the prostaglandin-producing red alga Gracilaria vermiculophylla by ammonium sulfate precipitation, anion-exchange chromatoraphy and gel filtration chromatography. Sodium dodecylsulfate– polyacrylamide gel electrophoresis of the final preparation showed a single band corresponding to a molecular mass of 20 kDa, but Superdex 200 fast protein liquid chromatography exhibited a molecular mass of 40 kDa. Accordingly, it was suggested that the purified enzyme was a homodimer of a 20 kDa subunit. The optimal temperature and pH were 37°C and 7–8, respectively. The purified enzyme catalyzed hydrolysis of the acyl groups of both glycoglycerolipids and phospholipids, especially monogalactosyldiacylglycerol and phosphatidylcholine. These results suggest that the enzyme hydrolyze the membrane lipids of the alga to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis. 相似文献
152.
Akihiko SUGIYAMA Jing SUN Kota UEDA Satoshi FURUKAWA Takashi TAKEUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):789-797
Six-day-old rats were treated intraperitoneal injections with methotrexate 1
mg/kg, and the cerebellum was examined. Both the length and width of the vermis decreased
in the methotrexate-treated group instead of the control from 4 day after treatment (DAT)
onward. A significant reduction in the width of the external granular layer was detected
on 2 and 3 DAT in the methotrexate group. By 4 DAT, the width of the external granular
layer of the methotrexate group was indistinguishable from the control, and by 8 DAT, it
was greater than that of the control. The molecular layer of methotrexate group on 8 and
15 DAT was thinner than that of the control. On 1 DAT, in the methotrexate group, there
were many TUNEL and cleaved caspase-3-positive granular cells throughout the external
granular layer, and they decreased time-dependently. On 1 DAT, in the methotrexate group,
phospho-histone H3-positive cells in the external granular layer were fewer than in the
control and tended to increase on 2–4 DAT. The p21-positive-rate of the external granule
cells in the MTX group was higher than in the control on 1–4 DAT. These results suggested
that methotrexate exposure on postnatal day 6 induces a delay, slowing in the migration of
external granular cells to the inner granular layer, attributed to decrease or inhibition
in the production of external granular cells that had arisen from apoptosis and the
decrease in cell proliferative activity, resulting in cerebellar hypoplasia. 相似文献
153.
Kimoto Y Sugiyama A Nishinohara M Asano A Masuda A Ochi T Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):403-407
The purpose of this study was to evaluate whether dityrosine and advanced oxidation protein products (AOPP) reflect the severity of cisplatin-induced nephrotoxicity. Immunoexpression of dityrosine in kidneys and plasma AOPP concentration were examined up to day 4 post-cisplatin injection in rats. Cisplatin injection induced tubular injury on days 2-4 after injection and increased serum creatinine and BUN on days 3 and 4. On days 2-4, dityrosine was immunostained in the cytoplasm of damaged tubular cells, and their immunostaining intensity increased time-dependently. Plasma AOPP levels were significantly increased on days 3 and 4. These results suggest that expressions of dityrosine and AOPP were associated with the severity of renal injury and may be useful markers for the development of cisplatin-induced nephrotoxicity. 相似文献
154.
Moritoh K Maeda A Nishino T Sasaki N Agui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):683-686
We have developed a West Nile virus (WNV) subgenomic replicon harboring the secreted alkaline phosphatase (SEAP) reporter gene instead of viral structural genes (designated repWNV/SEAP). The repWNV/SEAP allowed easy evaluation of viral replication efficiency by direct measurement of SEAP secretion in the cell culture medium in physical containment level 2 facilities. Furthermore, we validated the availability of this system using a known anti-flavivirus gene, mouse oligoadenylate synthetase 1b (Oas1b). The Oas1b-transfected cells were more resistant to repWNV/SEAP replication than the original cells. Thus, this system not only affords a useful tool for identification/evaluation of anti-flavivirus genes/drugs in terms of safety, ease of use and reliability, but should be able to reduce or replace the bioassay using laboratory animals. 相似文献
155.
Akihiko Okahara Hidetoshi Tanioka Koichi Takada Kouichi Kawazu 《Journal of toxicologic pathology》2013,26(4):343-349
This study was performed to assess the in vivo ocular toxicity of benzalkonium chloride (BAK) homologs compared with commercially available BAK (BAK mixture) and to assess the ocular toxicity of BAK homolog after repeated ocular application. Rabbit eyes were examined by ophthalmology and scanning electron microscopy (SEM) after 10 applications of BAK homologs with C12 (C12-BAK) and C14 (C14-BAK) alkyl chain lengths and a BAK mixture at concentrations of 0.001% (w/v), 0.003% (w/v), 0.005% (w/v), 0.01% (w/v) and 0.03% (w/v). The ocular toxicity of C12-BAK to rabbit eyes was examined by ophthalmology and histopathology after repeated ocular application for 39 weeks. In addition, the antimicrobial activities of C12-BAK and C14-BAK against A. niger, S. aureus and P. aeruginosa were assessed. Ocular toxicity of C12-BAK was less than those of the BAK mixture and C14-BAK. No ocular toxicity was noted after ocular application of 0.01% C12-BAK to rabbits for 39 weeks. C12-BAK showed antimicrobial activities at a concentration of 0.003%. These results suggest that the use of C12-BAK to replace BAK mixture as a preservative in ophthalmic solutions should be considered in order to reduce the incidence of the corneal epithelial cell injury induced clinically by BAK. 相似文献
156.
Yuka MORITA Naoshi HIRAMATSU Toshiaki FUJITA Haruna AMANO Etsuko KATSUMATA Kazutoshi ARAI Toshihide IWASAKI Takashi TODO Akihiko HARA 《The Journal of reproduction and development》2013,59(4):368-377
A single radial immunodiffusion (SRID) assay and a chemiluminescent immunoassay (CLIA)
were initially developed for alpha-fetoprotein (AFP) of the striped dolphin. Utilizing
these developed assays, we investigated pregnancy-associated changes in the levels of AFP
in the sera of fetuses and pregnant females of three dolphin species; samples were either
collected from captive individuals or obtained as fishery by-products. The concentrations
of AFP in the fetal serum ranged from 419.0 to 2026.3 μg/ml in the striped dolphin, 12.6
to 1218.7 μg/ml (for an AFP equivalent; eqAFP) in the common bottlenose dolphin and 770.6
to 3129.1 μg eqAFP/ml in the Risso''s dolphin. AFP levels decreased with increased fetal
size in fetuses over 20 cm in length. The concentrations of AFP in sera of pregnant
females ranged from 7.18 to 8068.7 ng/ml in the striped dolphin, 6.6 to 1241.1 ng eqAFP/ml
in the common bottlenose dolphin and 3.4 to 2868.7 ng eqAFP/ml in the Risso''s dolphin. The
levels in most pregnant females were equal to or lower than those found in males and
nonpregnant individuals, although a few pregnant females exhibited extremely high levels
(in the range of hundreds to thousands of nanograms per milliliter). Such high levels of
AFP were not observed during pseudopregnancy. To our knowledge, this is the first report
on basal profiles for serum AFP levels in small odontocetes. The profiles indicated that
AFP may play a significant role during embryonic development, although maternal levels do
not appear to be a diagnostic biomarker for monitoring pregnancy. 相似文献
157.
Satoshi Furukawa Seigo Hayashi Masayoshi Abe Souichiro Hagio Kota Irie Yusuke Kuroda Izumi Ogawa Akihiko Sugiyama 《Journal of toxicologic pathology》2013,26(1):83-88
Abstract: Background data during the gestation period were obtained from 128 Wistar Hannover GALAS rats and 26 Crl:CD(SD) pregnant rats in the control groups of our previous toxicity studies. The body weights of dams in the Wistar Hannover GALAS rats were significantly lower throughout the gestation period than those in the Crl:CD(SD) rats. In contrast, the time-dependent change in the body weight gain (%) of dams showed very similar trends in both strains. The mean number of live embryos/fetuses in the Wistar Hannover GALAS rats was 12.0, and was lower than that (14.5) in the Crl:CD(SD) rats. The placental weights gradually increased with pregnancy progression and reached a plateau on gestation day (GD) 19, although the embryo/fetal weights rapidly increased from GD 17 to GD 21. The embryo/fetal weights in the Wistar Hannover GALAS rats were significantly lower on only GD 21 than those in the Crl:CD(SD) rats. It is considered that this fetal weight difference between the strains develops during the fetal period, but not during the organogenesis period. In contrast, there were no differences in the placental weights between the two strains. Microscopically, the thickness of the labyrinth zone in the Wistar Hannover GALAS rats was thicker throughout the gestation period than that in the Crl:CD(SD) rats. 相似文献
158.
159.
160.
Kazuhito Ichii Masayuki Kondo Young-Hee Lee Shao-Qiang Wang Joon Kim Masahito Ueyama Hee-Jeong Lim Hao Shi Takashi Suzuki Akihiko Ito Hyojung Kwon Weimin Ju Mei Huang Takahiro Sasai Jun Asanuma Shijie Han Takashi Hirano Ryuichi Hirata Tomomichi Kato Sheng-Gong Li Ying-Nian Li Takahisa Maeda Akira Miyata Yojiro Matsuura Shohei Murayama Yuichiro Nakai Takeshi Ohta Taku M. Saitoh Nobuko Saigusa Kentaro Takagi Yan-Hong Tang Hui-Min Wang Gui-Rui Yu Yi-Ping Zhang Feng-Hua Zhao 《Journal of Forest Research》2013,18(1):13-20
Based on the model–data comparison at the eddy-covariance observation sites from CarboEastAsia datasets, we report the current status of the terrestrial carbon cycle modeling in monsoon Asia. In order to assess the modeling performance and discuss future requirements for both modeling and observation efforts in Asia, we ran eight terrestrial biosphere models at 24 sites from 1901 to 2010. By analyzing the modeled carbon fluxes against the CarboEastAsia datasets, the strengths and weaknesses of terrestrial biosphere modeling over Asia were evaluated. In terms of pattern and magnitude, the carbon fluxes (i.e., gross primary productivity, ecosystem respiration, and net ecosystem exchange) at the temperate and boreal forest sites were simulated best, whereas the simulation results from the tropical forest, cropland, and disturbed sites were poor. The multi-model ensemble mean values showed lower root mean square errors and higher correlations, suggesting that composition of multiple terrestrial biosphere models would be preferable for terrestrial carbon budget assessments in Asia. These results indicate that the current model-based estimation of terrestrial carbon budget has large uncertainties, and future research should further refine the models to permit re-evaluation of the terrestrial carbon budget. 相似文献