全文获取类型
收费全文 | 100篇 |
免费 | 10篇 |
专业分类
林业 | 1篇 |
农学 | 6篇 |
1篇 | |
综合类 | 3篇 |
畜牧兽医 | 94篇 |
园艺 | 1篇 |
植物保护 | 4篇 |
出版年
2023年 | 2篇 |
2020年 | 2篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2014年 | 6篇 |
2013年 | 6篇 |
2012年 | 2篇 |
2011年 | 7篇 |
2010年 | 2篇 |
2009年 | 8篇 |
2008年 | 1篇 |
2006年 | 1篇 |
2005年 | 1篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2001年 | 8篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1973年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有110条查询结果,搜索用时 656 毫秒
91.
MB Vieira I Bianchi EM Madeira VFB Roll CA Oliveira P Viau I Pivato NC Severo FAB Del Pino A Schneider MN Corrêa 《Reproduction in domestic animals》2010,45(6):1110-1113
This study aimed to evaluate the effect of the exogenous recombinant bovine somatotropin (rbST) on plasma concentrations of insulin‐like growth factor I (IGF‐I), insulin and semen quality of bulls. Twenty bulls (Aberdeen Angus and Brangus) were divided by breed into two groups. Placebo group was injected with NaCl 0.9% (s.c.) and treatment group with rbST (s.c., 500 mg) at days 0 and 14 of the experiment. Immediately after semen collection, blood samples were taken on days 0, 14, 28, 42 and 56 of the experiment. Semen was also collected on day 70 of the experiment. Evaluation of sperm motility was performed at pre‐freezing and post‐thawing stage, whereas assessment of sperm membrane integrity was performed after freezing and thawing. Analysis of data revealed that the effect of treatment and treatment‐by‐collection day on plasma concentrations of IGF‐I and insulin was not significant. However, mean plasma concentrations of IGF‐I and insulin were affected (p < 0.0001) by days of blood sampling. Effect of treatment and treatment‐by‐collection day on motility of spermatozoa was similar (p > 0.05) at pre‐freezing and post‐thawing stage. Intactness of plasmalemma and tail membrane of spermatozoa at post‐thawing stage was higher (p < 0.05) in rbST‐treated group than in control. In conclusion, rbST did not affect plasma concentrations of IGF‐I and insulin, however, it did improve post‐thaw sperm membrane integrity. 相似文献
92.
U. Flitsanov A. Mizrach A. Liberzon M. Akerman G. Zauberman 《Postharvest Biology and Technology》2000,20(3):279-286
The low-temperature storage of avocado affects its subsequent softening process and shelf life. One of the main indices of ripeness in avocado fruit is firmness, which changes during the ripening and softening process. The temperature and duration of storage fundamentally influence the firmness of the stored fruit, and monitoring the softening of fruit enables us to regulate its shelf life. The objective of the present study was to use nondestructive ultrasonic tests to elucidate the influences of storage temperature and time on the softening process of avocado fruit. The attenuation of the ultrasonic waves transmitted through the fruit tissue changes as the fruit passes through the various softening stages during and after low-temperature storage. Four groups of avocados, each stored at a different low temperature, and a control group which was stored at room temperature (20°C) were examined during and after their designated storage times, until they reached full ripeness at room temperature. Nondestructive ultrasonic tests and destructive penetration measurements were carried out in order to determine the attenuation and the tissue firmness, respectively, of the avocados. Statistical analysis showed quite good correlation between the firmness and the ultrasonic attenuation, and their dependence on previous storage time-temperature history. This suggests that the ultrasonic measurements could be used as a nondestructive method of monitoring avocado ripeness during low-temperature storage. 相似文献
93.
94.
Anneli Ritala Reino Aikasalo Kristian Aspegren Marjatta Salmenkallio-Marttila Satu Akerman Leena Mannonen Ulrika Kurtén Riitta Puupponen-Pimiä Teemu H. Teeri Veli Kauppinen 《Euphytica》1955,85(1-3):81-88
Summary Transgenic barley plants (Hordeum vulgare L. cv. Kymppi) were obtained by particle bombardment of various tissues. Immature embryos and microspore-derived cultures were bombarded with gold particles coated with plasmid DNA carrying the gene coding for neomycin phosphotransferase II (NPTII), together with plasmid DNA containing the gene for -glucuronidase (GUS).Bombarded immature embryos were grown to plants without selection and NPTII activity was screened in small plantlets. One plant proved to be transgenic (T0). This chimeric plant passed the transferred nptII gene to its T1 progeny. The presence of the nptII gene was demonstrated by the PCR technique and enzyme activity was analyzed by an NPTII gel assay. Four T0 spikes and 15 T1 offspring were transgenic. The integration and inheritance was confirmed by Southern blot hybridization. Transgenic T2 and T3 plants were produced by isolating embryos from green grains of transgenic T1 and T2 plants, respectively and growing them to plants. After selfing, the ratio of transgenic to non-transgenic T2 offspring was shown to follow the rule of Mendelian inheritance. The general performance of transgenic plants was normal and no reduction in fertility was observed.Microspore-derived cultures were bombarded one and four weeks after microspore isolation. After bombardment, cultures were grown either with or without antibiotic selection (geneticin
R
or kanamycin). When cultures were grown without selection and regenerated plants were transferred to kanamycin selection in rooting phase, one out of a total of about 1500 plants survived. This plant both carried and expressed the transferred nptII gene. The integration was confirmed by Southern blot hybridization. This plant was not fertile. 相似文献
95.
96.
A preliminary investigation comparing pre-operative morphine and buprenorphine for postoperative analgesia and sedation in cats 总被引:2,自引:1,他引:1
GW Stanway BVSC Cert VA PM Taylor MA Vet MB PhD DVA Dip ECVA & DC Brodbelt† MA Vet MB DVA Dip ECVA MRCVS 《Veterinary anaesthesia and analgesia》2002,29(1):29-35
Objective To compare the postoperative analgesic and sedative properties of buprenorphine and morphine in cats. Study Design Prospective, randomized, blinded study. Animals Thirty‐two domestic cats undergoing surgery. Methods Cats received pre‐anaesthetic medication with acepromazine (0.05 mg kg?1) given intramuscularly and were randomly allocated to group M and given morphine (0.1 mg kg?1) intramuscularly (IM) or to group B and given buprenorphine (0.01 mg kg?1) IM. Anaesthesia was induced with propofol and maintained with halothane in oxygen and nitrous oxide. Pain and sedation scores using visual analogue scales, and heart and respiratory rates, were measured immediately before, and 30, 60, 120, 180, 300 and 420 minutes after anaesthesia. Results Pain scores were significantly lower at 60, 120 and 180 minutes after anaesthesia in group B. Group B also had higher heart rates at 30 minutes. There were no other statistically significant differences between the groups. Clinical relevance Buprenorphine (0.01 mg kg?1) appeared to provide better postoperative analgesia than morphine (0.1 mg kg?1) and may also have a longer duration of action. 相似文献
97.
Adrian Boswood Vet MB Christopher R. Lamb Vet MB Daniel J. Brockman BVSC Panagiotis Mantis DVM Angela L. Witt DVM 《Veterinary radiology & ultrasound》2003,44(1):53-55
A cat was diagnosed with nasopharyngeal stenosis on the basis of clinical signs, survey and contrast radiography, endoscopy and magnetic resonance imaging. The condition was successfully managed with repeated balloon dilatation. 相似文献
98.
99.
Polly M Taylor MA VetMB PhD DVA MRCVS Stelio PL Luna DVM PhD Kate L White MA Vet MB CertVA MRCVS Malcolm Bloomfield Abigail L Fowden MA PhD 《Veterinary anaesthesia and analgesia》2001,28(3):119-125
Objective To characterize intravenous anaesthesia with detomidine, ketamine and guaiphenesin in pregnant ponies. Animals Twelve pony mares, at 260–320 days gestation undergoing abdominal surgery to implant fetal and maternal vascular catheters. Materials and methods Pre‐anaesthetic medication with intravenous (IV) acepromazine (30 µg kg?1), butorphanol (20 µg kg?1) and detomidine (10 µg kg?1) preceded induction of anaesthesia with detomidine (10 µg kg?1) and ketamine (2 mg kg?1) IV Maternal arterial blood pressure was measured directly throughout anaesthesia and arterial blood samples were taken at 20‐minute intervals for measurement of blood gases and plasma concentrations of cortisol, glucose and lactate. Anaesthesia was maintained with an IV infusion of detomidine (0.04 mg mL?1), ketamine (4 mg mL?1) and guaiphenesin (100 mg mL?1) (DKG) for 140 minutes. Oxygen was supplied by intermittent positive pressure ventilation (IPPV) adjusted to maintain PaCO2 between 5.0 and 6.0 kPa (38 and 45 mm Hg), while PaO2 was kept close to 20.0 kPa (150 mm Hg) by adding nitrous oxide. Simultaneous fetal and maternal blood samples were withdrawn at 90 minutes. Recovery quality was assessed. Results DKG was infused at 0.67 ± 0.17 mL kg?1 hour?1 for 1 hour then reduced, reaching 0.28 ± 0.14 mL kg?1 hour?1 at 140 minutes. Arterial blood gas values and pH remained within intended limits. During anaesthesia there was no change in heart rate, but arterial blood pressure decreased by 10%. Plasma glucose and lactate increased (10‐fold and 2‐fold, respectively) and cortisol decreased by 50% during anaesthesia. Fetal umbilical venous pH, PO2 and PCO2 were 7.34 ± 0.06, 5.8 ± 0.9 kPa (44 ± 7 mm Hg) and 6.7 ± 0.8 kPa (50 ± 6 mm Hg); and fetal arterial pH, PO2 and PCO2 were 7.29 ± 0.06, 4.0 ± 0.7 kPa (30 ± 5 mm Hg) and 7.8 ± 1.7 kPa (59 ± 13 mm Hg), respectively. Surgical conditions were good but four ponies required a single additional dose of ketamine. Ponies took 60 ± 28 minutes to stand and recovery was good. Conclusions and clinical relevance Anaesthesia produced with DKG was smooth while cardiovascular function in mare and fetus was well preserved. This indicates that DKG infusion is suitable for maintenance of anaesthesia in pregnant equidae. 相似文献
100.
Unusual Patterns of Serum Antibodies to Streptococcus Equi in Two Horses with Purpura Hemorrhagica 总被引:1,自引:1,他引:0
Sebastian E. Heath Vet MB MVSc Raymond J. Geor BVSc MVSc Henry Tabel DVM PhD Kathy Mcintosh DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1991,5(5):263-267
An enzyme-linked immunosorbent assay (ELISA) was developed for use in horses to determine serum titers of antibodies of the immunoglobulin classes IgA, IgG, and IgM to Streptococcus equi M-like protein and culture supernatant protein antigens. Serum antibodies were determined in 28 adult horses, including 9 horses with recent S. equi infections, 17 horses without known exposure to S. equi, but without a history of respiratory disease in the preceding 4 months, and 2 horses with clinical purpura hemorrhagica. Serum IgA titers to culture supernatant protein antigen were highest in recently infected horses (P less than 0.001). Serial determinations of antibody titers in the horses with purpura showed that IgG antibodies to both S. equi M-like protein and culture supernatant protein antigens were undetectable initially, but later rose coincidental with clinical recovery from the disease. Possible mechanisms for these findings are discussed. 相似文献