Analysis of landscape pattern change trajectories within areas of intensive agricultural use: case study in a watershed of southern Québec, Canada

This study aimed at capturing the spatial variability of landscape patterns and their trajectories of change from 1950 to 2000 within a watershed, which is representative of areas of intensive agricultural use. After an analysis of landscape features changes for the entire watershed based on aerial photographs, hierarchical clustering analysis provided a typology of landscape patterns for the cadastral lots. Following that, the trajectory of change of each lot was characterized (nature, importance, direction, rate of change). Seven types of landscape patterns are distinguished by the relative importance of different classes of landscape features and 51 trajectories of change were identified for the lots. The analysis shows that although the majority of lots were subjected to a homogenization of their landscape patterns since 1950, this trend is not entirely uniform and that since 2000 it occurs alongside trends towards diversification of certain landscape features on some lots. Furthermore, nearly a third of the lots are not following the main trajectories of change detected. Thus, the results suggest that extrinsic forces (policies, technologies) that are directing main changes in areas of intensive agricultural use toward uniformity could be modulated by internal forces (uses and values of the population). A better understanding of theses internal forces seems crucial to manage landscapes. From a methodology standpoint, although the hierarchical clustering analyses appear useful for understanding the spatial and temporal variability of landscape patterns, particular attention must be given to validating the typology chosen to characterize them.     

Channels and ditches as the last shelter for freshwater mussels: the case of Margaritifera auricularia and other naiads inhabiting the mid Ebro River Basin,Spain

• 1. Freshwater mussels or naiads are generally considered to thrive in river habitats, provided the ecological conditions are good. The presence of populations of these bivalves in artificial channels and ditches with natural bottoms has only scarcely been reported. The aim of this paper was to present the idea that these ‘channel’ and ‘ditch’ habitats could in fact be a sanctuary for naiads.
• 2. Approximately 80 km of several of these waterways fed by the mid Ebro River were sampled in Spain to monitor their naiad populations. Observations indicate that these habitats harbour substantial colonies of freshwater mussels (including two populations of adult specimens of the endangered species Margaritifera auricularia), much more so than the corresponding river.
• 3. The authors wish to alert conservation authorities and freshwater mussel experts to the extreme fragility and importance of this kind of habitat for the long‐term conservation of these imperilled molluscs.

Copyright © 2008 John Wiley & Sons, Ltd.     

Evaluating eddy covariance cotton ET measurements in an advective environment with large weighing lysimeters

Eddy covariance (EC) systems are being used to assess the accuracy of remote sensing methods in mapping surface sensible and latent heat fluxes and evapotranspiration (ET) from local to regional scales, and in crop coefficient development. Therefore, the objective was to evaluate the accuracy of EC systems in measuring sensible heat (H) and latent heat (LE) fluxes. For this purpose, two EC systems were installed near large monolithic weighing lysimeters, on irrigated cotton fields in the Texas High Plains, during the months of June and July 2008. Sensible and latent heat fluxes were underestimated with an average error of about 30%. Most of the errors were from nocturnal measurements. Energy balance (EB) closure was 73.2–78.0% for daytime fluxes. Thus, daylight fluxes were adjusted for lack of EB closure using the Bowen ratio/preservation of energy principle, which improved the resulting EC heat flux agreement with lysimetric values. Further adjustments to EC-based ET included nighttime ET (composite) incorporation, and the use of ‘heat flux source area’ (footprint) functions to compensate ET when the footprint expanded beyond the crop field boundary. As a result, ET values remarkably matched lysimetric ET values, with a ‘mean bias error ± root mean square error’ of −0.03 ± 0.5 mm day⁻¹ (or −0.6 ± 10.2%).     

Response of apricot trees to deficit irrigation strategies

During four growing seasons, 10-year-old apricot trees (Prunus armeniaca L., cv. ‘Búlida’) were submitted to three different drip irrigation regimes: (1) a control treatment, irrigated at 100% of seasonal crop evapotranspiration (ETc), (2) a continuous deficit irrigation (DI) treatment, irrigated at 50% of the control treatment, and (3) a regulated deficit irrigation (RDI) treatment, irrigated at 100% of ETc during the critical periods, which correspond to stage III of fruit growth and 2 months after harvest (early postharvest), and at 25% of ETc during the rest of the non-critical periods in the first two growing seasons and at 40% of ETc in the third and fourth. Soil–plant–water relation parameters were sensitive to the water deficits applied, which caused reductions in leaf and soil water potentials. The longer and severer deficits of the RDI treatment decreased fruit yield in the first two seasons. The RDI treatment pointed to two threshold values that defined the level at which both plant growth and yield were negatively affected with respect to the control treatment: (1) a predawn leaf water potential of around −0.5 MPa during the critical periods, and (2) a 22% drop in irrigation water. The total yield obtained in the DI treatment was significantly reduced in all the years studied due to the lower number of fruits per tree. No changes in the physical characteristics of fruits were observed at harvest. RDI can be considered a useful strategy in semiarid areas with limited water resources.     

Salmonid fish viruses and cell interactions at early steps of the infective cycle

A flow cytometric virus-binding assay that directly visualizes the binding and entry of infectious pancreatic necrosis virus (IPNV), infectious haematopoietic necrosis virus (IHNV) and virus haemorrhagic septicaemia virus (VHSV) to several cell lines was established. The highest efficiency of binding was shown by the BF-2 cell line and this was used to study, at the attachment level, the interactions of these cells with salmonid fish viruses in coinfections, and to further determine if the earliest stage of the viral growth cycle could explain the previously described loss of infectivity of IHNV when IPNV is present. Our results demonstrated that IPNV binds to around 88% of cells either in single or dual infections, whereas IHNV attachment always decreased in the presence of any of the other viruses. VHSV binding was not affected by IPNV, but coinfection with IHNV reduced the percentage of virus-binding cells, which suggests competition for viral receptors or co-receptors. Internalization of the adsorbed IHNV was not decreased by coinfection with IPNV, so the hypothetical competence could be restricted to the binding step. Treatment of the cells with antiviral agents, such as amantadine or chloroquine, did not affect the binding of IPNV and VHSV, but reduced IHNV binding by more than 30%. Tributylamine affected viral binding of the three viruses to different degrees and inhibited IPNV or IHNV entry in a large percentage of cells treated for 30 min. Tributylamine also inhibited IHNV cytopathic effects in a dose-dependent manner, decreasing the virus yield by 4 log of the 50% endpoint titre, at 10 mm concentration. IPNV was also inhibited, but at a lower level. The results of this study support the hypothesis that IHNV, in contrast to VHSV or IPNV, is less efficient at completing its growth cycle in cells with a simultaneous infection with IPNV. It can be affected at several stages of viral infection and is more sensitive to the action of antiviral compounds.     

Variability in conspecific predation among longnose lancetfish Alepisaurus ferox in the western Indian Ocean

ABSTRACT:   Conspecific predation among longnose lancetfish Alepisaurus ferox was investigated in four spatio-temporal strata of the western Indian Ocean. The cannibalism level varied from 0 to 45.5% by frequency of occurrence and was negatively related with abundance of non-evasive prey (such as crustaceans Charybdis smithii and Natosquilla investigatoris ) and foraging success. Predation by lancetfish is often described as a non-selective process, constrained by local prey availability and by its feeding speed during an attack of prey. Our results show that lancetfish may adapt its opportunistic foraging behavior, feeding on non-conspecific abundant prey such as crustaceans when available, and switching to a high level of conspecific predation in poor waters.     

Growth,absorption and assimilation efficiency by mature cuttlefish (<Emphasis Type="Italic">Sepia officinalis</Emphasis>) fed with alternative and artificial diets

The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish powder) to mature cuttlefish, Sepia officinalis, were analysed. Growth and feeding rates (GR and FR, % BW day⁻¹), food conversions (FC, %), and total protein and lipid composition of the diets were determined. Digestive gland to body weight ratio and absorption efficiency were calculated for each diet. Cuttlefish fed shrimp and crayfish grew larger (1.5 and 1.1% BW day⁻¹, respectively) compared to the other diets. Shrimp promoted the highest FC, followed by crayfish, and sardine. The highest FR was obtained for cuttlefish fed crayfish (8.4% BW day⁻¹). Although both artificial diets were accepted, none produced growth. A positive correlation (r = 0.96) between cuttlefish ingestion rate and digestive gland weight was obtained. Some cannibalism occurred among cuttlefish fed the artificial diets during the last week of the experiment. According to the results obtained, P. clarkii could be used as an alternative prey to shrimp for rearing adult mature (>50 g) S. officinalis.     

Insulin and IGF-I receptors and tyrosine kinase activity in carp ovaries: changes with reproductive cycle

Insulin and insulin-like growth factor I (IGF-I) receptors from carp ovaries were semipurified with wheat germ agglutinin at different moments of the reproductive cycle and their binding characteristics and tyrosine kinase activity were studied. Specific receptors for insulin and IGF-I were found. IGF-I receptors presented higher binding (23.8 ± 1.5%), number of receptors (965 ± 20fm/mg) and affinity (K_D 0.24 ± 0.03nM) than those shown for insulin receptors (4.1 ± 1%, 530 ± 85fm/mg and 0.85 ± 0.1nM, respectively). Insulin and IGF-I receptors have a tyrosine kinase activity which is not different from that found in muscle of the same species. Seasonal changes were found in binding, with maximum values for insulin and IGF-I reached at the end of pre-spawning period (June). However, while IGF-I binding was observed in all stages, insulin binding decreased in autumn and disappeared in winter, which suggests a different role for the two peptides in ovarian physiology.     

Effects of Broomrape Parasitism on Sunflower Plants: Growth,Development, and Mineral Nutrition

Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic plant that infects sunflower (Helianthus annuus L.) plants. In this work, sunflower plants were grown under greenhouse conditions in pots with the substrate infested or non-infested with broomrape seeds. At different numbers of days after sowing, plant height, internode lengths, number of leaves, head diameter, mineral composition of leaves, and potassium (K) concentration in stem were measured. The negative effects of broomrape parasitism were assessed from 57 d after sowing, when broomrape started to emerge. Parasitized plants exhibited lower shoot dry weight, height, and head diameter than control plants. The reduction in internode lengths was associated with a decrease in the gradient of K concentration from basal to apical stem. The mineral composition of leaves was also affected in parasitized plants. The concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), and zinc (Zn) in leaves of parasitized plants were lower than those of the control plants, while there were few differences for K, phosphorus (P), iron (Fe), and copper (Cu). The effects of parasitism are discussed in relation to their competition for resources and to perturbations of the host physiology such as hormonal and water balance.     

Herbivory of an invasive slug in a model grassland community can be affected by earthworms and mycorrhizal fungi

Invasion of non-native species is among the top threats for the biodiversity and functioning of native and agricultural ecosystems worldwide. We investigated whether the herbivory of the slug Arion vulgaris (formerly Arion lusitanicus; Gastropoda), that is listed among the 100 worst alien species in Europe, is affected by soil organisms commonly present in terrestrial ecosystems (i.e. earthworms—Annelida: Lumbricidae and arbuscular mycorrhizal fungi—AMF, Glomerales). We hypothesized that slug herbivory would be affected by soil organisms via altered plant nutrient availability and plant quality. In a greenhouse experiment, we created a simple plant community consisting of a grass, a forb, and a legume species and inoculated these systems with either two earthworm species and/or four AMF taxa. Slugs were introduced after plants were established. Earthworms significantly reduced total slug herbivory in AMF-inoculated plant communities (P?=?0.013). Across plant species, earthworms increased leaf total N and secondary metabolites, AMF decreased leaf thickness. Mycorrhizae induced a shift in slug feeding preference from non-legumes to legumes; the grass was generally avoided by slugs. AMF effects on legume herbivory can partly be explained by the AMF-induced increase in total N and decrease in C/N ratio; earthworm effects are less clear as no worm-induced alterations of legume plant chemistry were observed. The presence of earthworms increased average AMF colonization of plant roots by 140 % (P?<?0.001). Total shoot mass was significantly increased by AMF (P?<?0.001). These data suggest that the feeding behavior of this invasive slug is altered by a belowground control of plant chemical quality and community structure.