暴雨条件下灌木对边坡稳定性的影响模拟试验

[目的]研究暴雨条件下不同灌木种植模式对不同坡度边坡的固坡效应,探索灌木固坡发挥效用的临界坡度,为生态工程措施在山地灾害防治中的应用提供科学依据.[方法]通过制作灌木模型开展室内降雨试验,探究4种坡度(20°,35°,50°,60°)条件下4种灌木种植模式(稀植、适中种植、密植、裸坡)对边坡稳定性的影响,并利用考虑灌木...     

Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice

We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection.     

先锋植物沙棘Ⅱ--克隆行为和根系特化是实现沙棘先锋作用的又一根本因素

沙棘是根源性根出条型克隆植物,它有克隆习性和克隆行为,它具有有性和无性2种繁殖方式。在田间沙棘林内,株间、片断、斑块间,存在着种群遗传上的多样性。沙棘侧根的特化结构组成了天然生物蓄水库,缓解了在干旱条件下对水分需求的矛盾。因此,作为一种双重生态型植物,解决了肥(N2)、水2个重要的生命及生态因子,是实现先锋植物功能的重要原因。     

一类合作和竞争系统的全局渐近稳定性

利用负散度和不可约合作（竞争）系统的单调性质，分别研究二维和三维合作（竞争）系统的全局渐近稳定性．所得结果推广了已有文献的相关结论．     

甘草的利用研究进展

乌拉尔甘草、刺毛甘草、光果甘草、胀果甘草、刺果甘草等 5种甘草从资源分布及在医药、畜牧、食品、化工领域的应用等方面的研究进行了综述 ,对甘草的进一步开发利用提供了科学依据     

黄曲霉毒素B1液相色谱法检测技术比较分析

从柱前衍生、柱后衍生、荧光检测、紫外检测的分离检测技术和免疫亲和柱净化、多功能净化柱净化、硅镁吸附剂净化、液液萃取净化、硅胶净化、固相萃取等前处理技术方面对粮食中黄曲霉毒素B1的液相色谱检测技术进行了比较分析.     

部分商品化非洲猪瘟病毒荧光PCR检测试剂盒的比对

为获得商品化非洲猪瘟病毒(ASFV)荧光PCR检测试剂盒的敏感性、特异性、一致性和最低检测限等试验数据,以世界动物卫生组织(OIE)推荐引物探针为金标准,以62份已灭活的ASFV阳性田间样品、32份阴性田间样品和P72基因核酸标准物质为检测对象,对农业农村部公布许可的其中17个厂家生产的商品化ASFV荧光PCR检测试剂...     

IBRV重组gD蛋白的真核表达与间接ELISA检测方法的建立

通过优化牛传染性鼻气管炎病毒（IBRV）gD基因序列为Sf9细胞偏好密码子,转座形成穿梭载体,将质粒瞬时转染Sf9昆虫细胞,利用昆虫杆状病毒表达系统表达纯化重组IBRV gD蛋白;以制备的重组IBRV gD蛋白为包被抗原,建立检测IBRV血清中和抗体的间接ELISA方法,并通过比对检测已知血清中和抗体效价的牛血清,验证所建立的间接ELISA方法的敏感性、特异性与重复性等指标。结果显示：本研究建立的间接ELISA方法对相关的牛病毒血清抗体无交叉反应,敏感性可达1:512,组内和组间变异系数均低于10%;使用建立的间接ELISA检测方法结合血清中和试验,对480份临床血清样品进行检测,发现两者敏感性符合率为98.18%,特异性符合率为93.33%,总符合率为96.67%。结果表明,本研究建立的检测IBRV血清中和抗体的间接ELISA方法具有良好的特异性、敏感性和重复性,可开发为临床适应的检测试剂盒。     

滴灌水温对土壤和骏枣树体养分含量的影响

【目的】研究滴灌水温对骏枣林土壤和树体养分吸收的影响,为新疆南疆地区红枣栽培提供技术支持。【方法】在新疆南疆地区,以5年生骏枣为研究对象,以自然水温为对照（CK）,探讨不同滴灌水温（10,20,30,40 ℃）对骏枣林下0～10,10～20和20～40 cm土层土壤速效N、速效P和速效K及枣树各器官（果实、枣吊、二次枝和叶片）全N、全P、全K含量的影响。【结果】（1）20 ℃水温处理可以提高0～10,10~20 cm土层的速效P含量,较CK处理增加32.29%,16.92%;与CK处理相比,10 ℃水温处理可以提高20～40 cm土层的速效N含量和0～20 cm土层的速效K含量,30 ℃水温处理可以提高0～10 cm 土层的速效N含量和20～40 cm土层的速效P含量,40 ℃水温处理可以提高10～20 cm土层的速效N含量,但差异均未达到显著水平。（2）CK处理可以提高骏枣叶片的全N、P、K含量和二次枝的全N、全K含量。与CK处理相比,20 ℃水温处理可以提高骏枣果实中的全N、P、K含量及枣吊全N含量和二次枝的全P含量,其中果实中全K含量较CK显著增加了1.13 g/kg,其他差异均未达到显著水平。40 ℃水温处理可以提高枣吊的全P、全K含量,但差异均未达到显著水平。【结论】增温水可以提高土壤和树体的养分含量。     

Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway

Isaridin E, a cyclodepsipeptide isolated from the marine-derived fungus Amphichorda felina (syn. Beauveria felina) SYSU-MS7908, has been demonstrated to possess anti-inflammatory and insecticidal activities. Here, we first found that isaridin E concentration-dependently inhibited ADP-induced platelet aggregation, activation, and secretion in vitro, but did not affect collagen- or thrombin-induced platelet aggregation. Furthermore, isaridin E dose-dependently reduced thrombosis formation in an FeCl₃-induced mouse carotid model without increasing the bleeding time. Mechanistically, isaridin E significantly decreased the ADP-mediated phosphorylation of PI3K and Akt. In conclusion, these results suggest that isaridin E exerts potent antithrombotic effects in vivo without increasing the risk of bleeding, which may be due to its important role in inhibiting ADP-induced platelet activation, secretion and aggregation via the PI3K/Akt pathways.