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991.
JOHN P. CARON DVM MVSc Diplomate ACVS CAROLE A. BOLIN DVM PhD JOSEPH G. HAUPTMAN DVM MS Diplomate ACVS KIMBERLY A. JOHNSTON VMD 《Veterinary surgery : VS》2009,38(5):664-669
Objective— To report the minimum inhibitory concentration (MIC) of amikacin sulfate for equine clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and characterize the initial kill and duration of the postantibiotic effect (PAE) for selected strains.
Study Design— Experimental study.
Methods— Isolates of MRSA (n=35) had their amikacin MIC determined using the E-test agar diffusion method. Two isolates with MICs>256 μg/mL limit were further characterized using broth macrodilution. Six distinct isolates with amikacin MICs of 32, 48, 128 (2 isolates) and 500 (2 isolates) μg/mL had PAE determinations made over a range of amikacin concentrations from 31.25–1000 μg/mL using standard culture-based techniques.
Results— Median MIC of the 35 isolates was 32 μg/mL (range 2 to >256 μg/mL). Mean PAE of selected MRSA strains had an overall mean (all amikacin doses) of 3.43 hours (range 0.10–9.57 hours). PAE for MRSA exposed to amikacin at 1000 μg/mL was 6.18 hours (range 3.30–9.57 hours), significantly longer than that for all other concentrations ( P <.0001). There was no statistically significant effect of isolate MIC on PAE.
Conclusions— Isolates had a wide range of MIC; however, growth of all 6 selected strains were inhibited within the range of concentrations tested, including 2 strains with MICs of 500 μg/mL. PAE duration was not influenced by the MIC of amikacin but was significantly longer with treatment at 1000 μg/mL than at lower concentrations.
Clinical Relevance— Clinical isolates of MRSA are susceptible to amikacin at concentrations achieved by regional perfusion: however, the modest duration of PAE observed suggest that further laboratory and in vivo evaluation be conducted before recommending the technique for clinical use. 相似文献
Study Design— Experimental study.
Methods— Isolates of MRSA (n=35) had their amikacin MIC determined using the E-test agar diffusion method. Two isolates with MICs>256 μg/mL limit were further characterized using broth macrodilution. Six distinct isolates with amikacin MICs of 32, 48, 128 (2 isolates) and 500 (2 isolates) μg/mL had PAE determinations made over a range of amikacin concentrations from 31.25–1000 μg/mL using standard culture-based techniques.
Results— Median MIC of the 35 isolates was 32 μg/mL (range 2 to >256 μg/mL). Mean PAE of selected MRSA strains had an overall mean (all amikacin doses) of 3.43 hours (range 0.10–9.57 hours). PAE for MRSA exposed to amikacin at 1000 μg/mL was 6.18 hours (range 3.30–9.57 hours), significantly longer than that for all other concentrations ( P <.0001). There was no statistically significant effect of isolate MIC on PAE.
Conclusions— Isolates had a wide range of MIC; however, growth of all 6 selected strains were inhibited within the range of concentrations tested, including 2 strains with MICs of 500 μg/mL. PAE duration was not influenced by the MIC of amikacin but was significantly longer with treatment at 1000 μg/mL than at lower concentrations.
Clinical Relevance— Clinical isolates of MRSA are susceptible to amikacin at concentrations achieved by regional perfusion: however, the modest duration of PAE observed suggest that further laboratory and in vivo evaluation be conducted before recommending the technique for clinical use. 相似文献
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Brachycephalic feline noses: CT and anatomical study of the relationship between head conformation and the nasolacrimal drainage system 总被引:1,自引:1,他引:0
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Retrospective evaluation of 155 adult equids and 21 foals with tetanus in Western,Northern, and Central Europe (2000–2014). Part 1: Description of history and clinical evolution 下载免费PDF全文
Gaby van Galen DVM PhD DECEIM DECVECC Claude Saegerman DVM PhD DECVPH Joke Rijckaert DVM Helene Amory DVM PhD DECEIM Lara Armengou DVM PhD DECEIM Barbora Bezdekova DVM PhD DECEIM Inge Durie DVM DECEIM Rikke Findshøj Delany DVM Nathalie Fouché DVM DVM Laura Haley MVB Michael Hewetson BVSc DECEIM Rene van den Hoven DVM PhD DECEIM Anna Kendall DVM DECEIM Fernando Malalana DVM DECEIM MRCVS Jessika Muller Cavalleri DVM DECEIM PhD Tresemiek Picavet DVM DECEIM PhD Katja Roscher DVM DECEIM Denis Verwilghen DVM PhD DECVS Meret Wehrli Eser DVM DECEIM Cornélie Westermann DVM PhD DECEIM Tim Mair BVSc PhD DECEIM 《Journal of Veterinary Emergency and Critical Care》2017,27(6):684-696
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The influence of Ringer's lactate or HES 130/0.4 administration on the integrity of the small intestinal mucosa in a pig hemorrhagic shock model under general anesthesia 下载免费PDF全文
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Ultrasound‐guided intracardiac xenotransfusion of canine packed red blood cells and epinephrine to the left ventricle of a severely anemic cat during cardiopulmonary resuscitation 下载免费PDF全文