Variation in sucrose esters from the type B glandular trichomes of certain wild potato species

Accessions of four insect resistant wild potato species (i.e., S. berthaultii,S. neocardenasii, S. polyadenium andS. tarijense) were examined for the presence of glandular trichomes and any associated sucrose esters. Of the thirty accessions examined, ten proved positive for the presence of type B glandular trichomes and accompanying sucrose esters. Three distinct complexes of sucrose esters were identified (i.e., 3,4,6-tri-0, 3,3′,4,6-tetra-0-, and 2,3,3′,4-tetra-0-acylated) and the acyl substituents were found to vary significantly within each complex. Possible rationales for the observed distribution of the sucrose esters within the species are discussed and comparative data from some non-tuberousSolanums and other genera of the Solanaceae family are presented.     

In vivo evaluation of vaccine efficacy against challenge with a contemporary field isolate from the α cluster of H1N1 swine influenza virus

Influenza A virus vaccines currently contain a mixture of isolates that reflect the genetic and antigenic characteristics of the currently circulating strains. This study was conducted to evaluate the efficacy of a trivalent inactivated swine influenza virus vaccine (Flusure XP) in pigs challenged with a contemporary α-cluster H1N1 field isolate of Canadian swine origin. Pigs were allocated to vaccinated, placebo, and negative-control groups and monitored for respiratory disease for 5 d after challenge. On the challenge day and 5 d after challenge the serum of the vaccinated pigs had reciprocal hemagglutination inhibition antibody titers 40 for all the vaccine viruses but ≤ 20 for the challenge virus. Gross lesions were present in the lungs of all pigs that had been inoculated with the challenge virus, but the proportion of lung tissue consolidated did not differ significantly between the placebo and vaccinated pigs. However, the amount of virus was significantly reduced in the nasal secretions, lungs, and bronchoalveolar lavage fluid in the vaccinated pigs compared with the placebo pigs. These results indicate that swine vaccinated with Flusure XP were partially protected against experimental challenge with a swine α-cluster H1N1 virus that is genetically similar to viruses currently circulating in Canadian swine.     

Mechanisms of aquatic therapy and its potential use in managing equine osteoarthritis

Aquatic therapy has become increasingly popular in its use for rehabilitation of equine musculoskeletal injuries. Unfortunately, there has been no scientific evaluation of its clinical application for the treatment of osteoarthritis (OA) or associated musculoskeletal injuries in horses. The purpose of this review is to describe mechanisms of action of aquatic therapy and its potential use in the clinical management of equine OA.     

Best practices for veterinary toxicologic clinical pathology,with emphasis on the pharmaceutical and biotechnology industries

The purpose of this paper by the Regulatory Affairs Committee (RAC) of the American Society for Veterinary Clinical Pathology (ASVCP) is to review the current regulatory guidances (eg, guidelines) and published recommendations for best practices in veterinary toxicologic clinical pathology, particularly in the pharmaceutical and biotechnology industries, and to utilize the combined experience of ASVCP RAC to provide updated recommendations. Discussion points include (1) instrumentation, validation, and sample collection, (2) routine laboratory variables, (3) cytologic laboratory variables, (4) data interpretation and reporting (including peer review, reference intervals and statistics), and (5) roles and responsibilities of clinical pathologists and laboratory personnel. Revision and improvement of current practices should be in alignment with evolving regulatory guidance documents, new technology, and expanding understanding and utility of clinical pathology. These recommendations provide a contemporary guide for the refinement of veterinary toxicologic clinical pathology best practices.     

Successful fostering of orphan lambs by nonpregnant ewes induced to lactate using exogenous hormone treatment

Six nonpregnant ewes were induced to lactate by an exogenous hormone treatment lasting 6 weeks. At the end of the treatment one alien lamb was adopted by each ewe. Each ewe was initially indifferent to the alien lamb and required only mild restraint to allow the lambs to suck successfully. Maternal interest subsequently increased and strong ewe-lamb bonding was evident by 80 minutes (n=l), 10 hours (n=4) or 24 hours (n=l) in different cases. The lambs required supplementary milk for 4–6 days while milk production by the ewes increased to adequate levels. Thereafter they were reared entirely by the ewes. Another four nonpregnant ewes were induced to lactate by a similar hormone treatment and were then hand-milked thrice daily for 2-3 weeks. One alien lamb was then introduced to each ewe. All four ewes were aggressive towards the lambs which were removed after 10–30 minutes. The ewes were then injected subcu- taneously with 5 mg of oestradiol-17β and were placed in fostering stocks. After a further 3–5 hours the lambs were re-introduced. Strong ewe-lamb bonding occurred during the subsequent 10 hours in all cases. All 10 lambs were reared successfully by these ewes until at least 3 months of age, when observations stopped. It was concluded firstly that nonpregnant ewes induced to lactate artificially will adopt and rear orphan lambs successfully, provided that additional milk is given to meet shortfalls during the first 4–6 days; and secondly that a 5 mg injection of oestradiol-17β may facilitate fostering of lambs by ewes in established lactation whether the lactation is induced artificially or not.     

A condition resembling Chastek paralysis in cats

Abstract

Extract

The design of a national mastitis survey, the methods used and the results have been described in Parts 1 and 2 of this series (Elliott et al., 1976 Elliott, R. E. W., Tattersfield, Jean G. and Brookbanks, E. O. 1975. New Zealand National Mastitis Survey: 1965–6. 1. Preliminary Studies. N.Z. vet. J., 24: 18–20.  [Google Scholar]; Tattersfield et al., 1976 Tattersfield, Jean G., Elliott, R. E. W. and Brookbanks, E. O. 1976. New Zealand National Mastitis Survey: 1965–6. 2. Measures of Mastitis Prevalence. N.Z. vet. J., 24: 40–54. [Taylor &; Francis Online] , [Google Scholar]).