Assessing How the Ratio of Barley Mash to Wood Chips in Compost Affects Rates of Microbial Processing and Subsequent Vegetable Yield

The composting of food waste coupled with urban agriculture presents an opportunity to increase nutrient recycling in urban ecosystems. One potential constraint limiting the expansion of aerobic food waste composting is the availability of carbon-rich recalcitrant materials, such as wood chips. We measured the differences in nutrient retention throughout the compost life cycle for different mixtures of barley mash to wood chips, to assess whether composting using proportionally less wood chips would lead to higher nutrient recycling rates. Nine compost piles (1 m³) were constructed at varying ratios barley mash to wood chips, ranging from 10:90 to 90:10. During the composting process, the 50:50 mixture maintained internal temperatures above 55°C for 30 days, with drop-offs as mixtures diverged in either direction. Food waste content was positively related to internal moisture and CO₂, and negatively related to internal O₂, throughout the ensuing 3 months. The finished compost was used in raised-bed garden plots during the following summer. Yields of arugula and tomatoes increased with compost barley mash content, saturating at high levels. Across all treatments, <5% of N and <2% of P were recycled from barley mash into new vegetable production. Although the maximum amount of nutrients was recycled using high barley mash compost, these treatments also had lower nutrient recycling efficiency compared to intermediate mixtures. These results indicate that the use of wood chips in composting increases the efficiency nutrient retention from food waste and in turn enhances nutrient recycling in urban environments.     

DNA markers and pollen morphology reveal that <Emphasis Type="Italic">Praecitrullus fistulosus</Emphasis> is more closely related to <Emphasis Type="Italic">Benincasa hispida</Emphasis> than to <Emphasis Type="Italic">Citrullus</Emphasis> spp.

The round melon Praecitrullus fistulosus (Stocks) Pangalo has been cultivated in Asia since ancient times and has been considered an underexploited crop in the western world. In the USA, there is an increased interest in using P. fistulosus as a commercial vegetable, and possibly as a rootstock for grafting watermelon, melon, or cucumber. However, the taxonomic classification of P. fistulosus is incomplete and for many years it has been considered a close relative of watermelon [Citrullus lanatus subsp. vulgaris (Schrad. ex Eckl. et Zeyh.) Fursa] and was previously classified as Citrullus lanatus subsp. fistulosus (Stocks) Duthie et J.B. Fuller. Here, we used two sets of DNA markers to assess the genetic similarity of P. fistulosus in relation to Citrullus spp. {including Citrullus lanatus subsp. vulgaris, C. lanatus subsp. lanatus, Citroides group [also known as C. lanatus (Thunb.) Matsum. et Nakai subsp. lanatus var. citroides (Bailey) Mansf. ex Greb.], and C. colocynthis (L.) Schrad.}, Cucumis spp. (including C. melo, C. sativus, C. anguria, C. meeusei, C. zeyheri), Benincasa hispida (Thunb.) Cogn., Lagenaria siceraria (Mol.) Standl. and Cucurbita spp. (including C. moschata Duchesne and the winter squash C. maxima Duchesne). The first marker set comprised 501 markers that were produced by 38 primer pairs derived from watermelon expressed sequenced tags (ESTs) containing simple sequence repeat (SSR) motifs (designated as EST-SSR primers; produced 311 markers), and by 18 primer pairs derived from ESTs that do not contain SSR motives (designated here as EST-PCR primers; produced 190 markers). The second marker set comprised 628 markers that were produced by 18 sequence related amplified polymorphism (SRAP) primer pairs. The phylogenetic data indicated that among these cucurbit species, the wax gourd B. hispida is the closest to the P. fistulosus. Pollen observations, using light microscopy, indicated that each of the cucurbit genera examined here has unique pollen morphology. The Cucurbita spp. have globular pollen grains with a stigmatic surface. The L. siceraria has polygonal pollen grains with symmetrical boundaries, while the Citrullus spp. and Cucumis spp. have ovular (conical) and triangular shaped pollen grains (respectively). The B. hispida and P. fistulosus have spherical or semispherical pollen grains. These pollen features appear to be in agreement with the phylogenetic relationships of these two species based on DNA markers. Analysis with 12 SRAP primer pairs revealed low genetic diversity among 18 United States Plant Introductions (PIs) of P. fistulosus, indicating the need to expand the germplasm collection of this cucurbit crop.     

Integrated data analysis in the presence of emigration and mark loss

Integrated data analyses are becoming increasingly common in studies of wild animal populations where two or more separate sources of data contain information about common parameters. These types of analyses provide robust parameter estimates which fully reflect all available information, as well as estimates of parameters that would be unidentifiable in a separate analysis. In this article we present an integrated Bayesian analysis of four long-term datasets (counts, two mark-recapture-recovery time series, and productivity) relating to a colony of common guillemots (Uria aalge) on the Isle of May, southeast Scotland. A complication when considering the dynamics of populations of this kind is the unobservable emigration of immature animals. In the analysis of mark-recapture-recovery data, the rate of emigration is frequently confounded with that of mark loss and it is only possible to estimate the product of these parameters. By combining all available data for the Isle of May guillemots in an integrated population model, we are able to estimate these parameters separately and thus obtain improved estimates of prerecruitment emigration.     

Computer simulation of the interactions of glyphosate with metal ions in phloem

Essential nutrients such as trace metal ions, amino acids, and sugars are transported in the phloem from leaves to other parts of the plant. The major chelating agents in phloem include nicotianamine, histidine, cysteine, glutamic acid, and citrate. A computer model for the speciation of metal ions in phloem has been used to assess the degree to which the widely used herbicide glyphosate binds to Fe(3+), Fe(2+), Cu(2+), Zn(2+), Mn(2+), Ca(2+), and Mg(2+) in this fluid over the pH range of 8 to 6.5. The calculations show that glyphosate is largely unable to compete effectively with the biological chelating agents in phloem. At a typical phloem pH of 8, 1.5 mM glyphosate binds 8.4% of the total Fe(3+), 3.4% of the total Mn(2+), and 2.3% of the total Mg(2+) but has almost no effect on the speciation of Ca(2+), Cu(2+), Zn(2+), and Fe(2+). As the pH decreases to 6.5, there are some major shifts of the metal ions among the biological chelators, but only modest increases in glyphosate binding to 6% for Fe(2+) and 2% for Zn(2+). The calculations also indicate that over 90% of the glyphosate in phloem is not bound to any metal ion and that none of the metal-glyphosate complexes exceed their solubility limits.     

Cladoceran Response to Calcium Decline and the Subsequent Inadvertent Liming of a Softwater Canadian Lake

Lake water calcium (Ca) decline has recently been recognized as a stressor impacting softwater lake districts that have experienced long-term patterns of acid deposition and/or timber harvesting. Declining aqueous Ca levels may impact the survival of aquatic biota, particularly Ca-rich cladoceran taxa such as daphniids. Daphnia pulex are sensitive to laboratory Ca levels below 1.5?mg?l^?1; however, responses of cladoceran communities to Ca decline in natural environments require further study. Dickie Lake (Ontario, Canada) is the site of an inadvertent natural experiment, providing insight into the effects of changing aqueous Ca availability upon cladoceran communities, as the lake has a history of acidification, followed by recent (1990s) Ca additions to the watershed via applications of calcium-rich road dust suppressants. Paleolimnological analyses were used to examine changes in cladoceran community structure (with a focus upon Ca-rich daphniids) from pre-industrial times to present day. Three distinct temporal stages were apparent in Dickie Lake??s daphniid community: 1870?C1950, 1950?C1990, and 1990?Cpresent. The daphniid community of the pre-industrial assemblages was dominated by members of the Daphnia longispina species complex, but shifted in the late 1950s to more acid- and Ca-insensitive members of the D. pulex species complex. During the most recent stage, coincident with dust suppressant applications, both daphniid complexes are well represented. Observed transitions between daphniid species complexes provide further evidence of the influence of Ca availability upon cladoceran community structure, indicating the potential importance of the controlled addition of Ca to freshwater systems (i.e., liming) as a mitigation/recovery strategy as Ca declines continue.     

Wildlife hunting practices and bushmeat dynamics of the Banyangi and Mbo people of Southwestern Cameroon

The Banyang-Mbo Wildlife Sanctuary, an area of lowland tropical rainforest in southwestern Cameroon, was designed by the Government of Cameroon specifically to protect 10 species and one order (Chelonia) of wildlife including some of the most endangered primates in Central and West Africa such as the drill (Mandrillus leucophaeus) and chimpanzee (Pan troglodytes). The long-settled local communities surrounding the Sanctuary maintain user rights of the forest and its natural resources as long as the conservation goals of the protected area are not compromised. A three-year study assessed local communities’ hunting dynamics in and around the sanctuary. Ten village-based assistants weighed, sexed, and aged all animal carcasses harvested by the 96 hunters participating in the study from June 1999 to March 2002. Data on weapon type, use of meat, location of kill, and price for each killed animal were also collected. We compare bushmeat dynamics and hunting cultures of two tribes living on the periphery of the Sanctuary, the Banyangi and the Mbo. A total of 13 489 animals were harvested during the study period, 4656 by Banyangi and 8833 by Mbo. Our results suggest that the condition of bushmeat when sold by hunters, either fresh or smoked, does not appear to affect the price. Bushmeat was significantly cheaper than the alternative sources of protein available in the nearest town. Although both tribes appear to use similar weapon technologies and hunting techniques, they differ with respect to amounts harvested and bushmeat use. The tribe settled deep in the forest with no road access hunts more intensively and for commercial sale outside the region, while the tribe along the road consumes or sells most of their catch locally.     

Dietary intake and development of a quantitative food-frequency questionnaire for the Barbados National Cancer Study

OBJECTIVE: To develop a quantitative food-frequency questionnaire (QFFQ) for the Barbados National Cancer Study (BNCS) that will permit examination of associations of diet with breast and prostate cancer. DESIGN: Population intake data from the year 2000 Barbados Food Consumption and Anthropometric Surveys (BFCAS 2000) were used to derive a list of foods consumed by the population. A 192-item draft QFFQ was developed from this list. SETTING: Barbados, West Indies provides an ideal environment to understand cancer risk in African-origin populations, with high relevance to African-Americans. The BNCS is a population-based case-control study examining risk factors for breast and prostate cancer in such populations. SUBJECTS: A total of 1600 persons, 18 years and older, completed a 24-hour recall in the BFCAS 2000. Fifty of 63 randomly selected residents (79% response rate) gave additional updated information on foods consumed. RESULTS: The 50 participants provided a one-time 24-hour recall and completed the draft QFFQ. The final instrument contains 148 items: breads, cakes, cereals = 17; rice, pastas, noodles = 8; dairy = 10; meat, fish, poultry = 42; fruit = 16; vegetables = 26; soft drinks = 14; alcoholic beverages = 5; others = 10. Additional questions include supplement use and food preparation methods such as grilling. CONCLUSION: The final instrument is concise, complete and the most up-to-date for assessing the food and nutrient intake of African-origin Barbadians and the associations with breast and prostate cancer.     

Predicting perchlorate exposure in milk from concentrations in dairy feed

Perchlorate has been detected in U.S. milk samples from many different states. Applying data from a recently reported 9-week experiment in which 16 Holstein dairy cows were administered perchlorate allowed us to derive an equation for the dose-response relationship between perchlorate concentrations in feed/drinking water and its appearance in milk. Examination of background concentrations of perchlorate in the total mixed ration (TMR) fed in addition to the variable dose supplied to treated cows as a ruminal infusate revealed that cows receive significant and variable exposure to perchlorate from the TMR. Weekly examination of the TMR disclosed that a change in ingredients midway through the experiment caused a significant (78%) change in TMR perchlorate concentration. Analyses of the ingredients comprising the TMR revealed that 41.9% of the perchlorate came from corn silage, 22.9% came from alfalfa hay and 11.7% was supplied by sudan grass. Finally, USDA Food and Nutrition Survey data on fluid milk consumption were used to predict potential human exposure from milk that contained concentrations of perchlorate observed in our previous dosing study. The study suggests that reducing perchlorate concentration in dairy feed may reduce perchlorate concentrations in milk as well as the potential to reduce human exposure to perchlorate in milk.     

Root traits of different crops under rainfed conditions in the High Barind Tract of Bangladesh

Root traits of six different crops grown on residual soil moisture in the post–rainy season in the High Barind Tract (HBT) of Bangladesh were investigated to better understand their adaptation to this moisture‐limited environment. Deep‐rooting chickpea is the currently favored rainfed crop grown after rainy‐season rice in the HBT, but it is necessary to identify alternative crops to chickpea in order to avoid buildup of pests and diseases. Averaged over 2 y, barley (1.72 Mg ha^–1) produced significantly more grain than chickpea (1.4 Mg ha^–1) which, in turn, yielded better than linseed (1.0 Mg ha^–1), wheat (0.93 Mg ha^–1), and mustard (0.77 Mg ha^–1). Lentil did not produce any grain at all. Grain yield for all crops increased as total root length increased above a threshold value of 0.05 to 0.1 km m^–2. In general, grain yield increased as the proportion of total root produced below 60 cm depth increased, although barley also had thin roots that could more effectively extract soil moisture. Expression of root traits varied considerably between seasons, which was attributable to the different rainfall patterns and bulk‐density characteristics of the soil profile in the 2 years of the study. Although favorable root traits, particularly rooting ability below 60 cm, are a prerequisite for acceptable yield levels of crops grown on residual soil moisture in the HBT, it is recognized that farmers' choice of a post‐rice crop will depend on its economic return or food‐security value.     

Maize and pea germination and seedling growth responses to compost generated from biowaste of selected invasive alien plant species

Plant biowaste of alien species represents a potential resource for compost production. This study investigated the seed and seedling responses of maize and pea to composts generated from the biowaste of four invasive species in eThekwini, South Africa: Acacia podalyriifolia, Hedychium gardnerianum, Litsea glutinosa, and Tithonia diversifolia. Except for a 40% concentration of T. diversifolia, leachates of the biowaste from the four species had no marked effects on germination. In seedling growth studies, Berea Red soil (control) was supplemented with composts produced using combinations of the four species (A. podalyriifolia + T. diversifolia [T1], A. podalyriifolia + H. gardnerianum [T2], L. glutinosa + T. diversifolia [T3], and L. glutinosa + H. gardnerianum [T4]), and a commercial compost (T5). Carbon and nitrogen levels of the biowaste composts were higher than the control, while their associated C/N ratios were low enough to encourage microbial growth, facilitate rapid decomposition, and support plant growth. A comparison of percentage seedling production, growth rate, and biomass production between the commercial compost and alien biowaste treatments revealed all parameters to be statistically comparable among T5, T1, and T3 for maize, and between T5 and T1 for pea. These superior biowaste composts did not enhance growth relative to the commercial compost, but supported growth to the same extent. However, N and P levels in T1 and T3 were lower than the commercial compost and appear to have altered biomass allocation patterns in both species relative to the commercial compost. The results suggest that there is potential to use invasive alien plant biowaste to improve soil for agricultural purposes.