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Javadi S Slingerland LI van de Beek MG Boer P Boer WH Mol JA Rijnberk A Kooistra HS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(5):625-631
A pathogenetic role of the renin-angiotensin-aldosterone system has been implicated in cats in both systemic arterial hypertension and hypokalemic myopathy. Yet, measurement of plasma aldosterone concentrations (PACs) and plasma renin activity (PRA) has not unequivocally pointed to hyperaldosteronism as a cause of these conditions. To obtain appropriate reference ranges, this study included a large number (130) of healthy house cats of different breeds without a history of recent illness and plasma concentrations of urea and creatinine below the upper limit of the respective reference ranges. In addition, the pituitary-adrenocortical axis was studied by measuring plasma concentrations of adrenocorticotropic hormone (ACTH), alpha-melanocyte-stimulating hormone (alpha-MSH), and cortisol. Reference ranges for PACs (110-540 pmol/L; 40-195 pg/mL), PRA (60-630 fmol/L/s; 0.3-3 ng/mL/h), and the aldosterone to renin ratio (ARR) (0.3-3.8) were very similar to those established in the same laboratory for humans in a supine position. No breed differences were found. The ARRs in neutered cats were significantly higher than in intact cats, primarily because of low PRA in neutered cats. The ARRs of cats > or = 5 years of age were significantly higher than those of cats < 5 years of age. The plasma concentrations of ACTH, alpha-MSH, and cortisol did not correlate significantly with PAC. Thus, although blood sampling was performed in cats in nonstandardized positions and was associated with a wide variation of stress responses, the references ranges of PAC, PRA, and ARR were similar to the relatively narrow limits established for humans under standardized conditions. The effects of neutering and aging on PRA and ARR warrant further investigation. 相似文献
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Mandimika T Baykus H Poortman J Garza C Kuiper H Peijnenburg A 《Journal of agricultural and food chemistry》2008,56(18):8745-8752
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Berkeveld M Langendijk P Verheijden JH Taverne MA van Nes A van Haard P Koets AP 《Journal of animal science》2008,86(12):3440-3449
The objective of the current study was to investigate whether plasma citrulline or intestinal fatty acid-binding protein (I-FABP) concentrations might be used as longitudinal markers for small intestinal function in piglets after weaning. Plasma citrulline and I-FABP concentrations were measured longitudinally in weaned and unweaned piglets, and related to intestinal absorption values (i.e., plasma mannitol and 3-xylose concentrations in a sugar absorption test). Within each litter (n = 10), 2 piglets with a close-to-litter-average BW were selected. At 20.8 +/- 0.4 d of age, the selected piglets per litter were either weaned conventionally (CW) or remained with the sow (UNW). One day before, and 0.5, 2, 4, and 7 d after weaning of the CW piglets, the selected piglets of both groups were subjected to a sugar absorption test. After a 2-h fast, piglets were administered an oral dose of 2 mL/kg of sugar solution, containing 50 mg/kg of mannitol and 100 mg/kg of 3-xylose. One hour after administration, a blood sample was collected from a jugular vein for determination of plasma I-FABP, citrulline, mannitol, and 3-xylose concentrations. Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the CW and UNW treatments (P = 0.63). The absorption of 3-xylose was not different between treatments (P = 0.83). Mannitol absorption, however, was less in the weaned CW piglets compared with the UNW piglets (P = 0.003), with the nadir on d 4 postweaning. Weaning also reduced plasma citrulline concentrations in the CW treatment compared with the UNW treatment (P < 0.001). On d 4 and 7 postweaning, plasma citrulline concentrations of CW piglets were less (P < 0.001 and P = 0.0013) than preweaning values. Furthermore, in the CW treatment, plasma citrulline concentrations correlated with plasma mannitol concentrations at d 4 postweaning (r = 0.89, P = 0.008) and overall (r = 0.76, P = 0.001). Based on these results, plasma citrulline concentration seems to be a possible marker for monitoring intestinal function in pigs after weaning. 相似文献
15.
Jules Beekwilder Adèle van Houwelingen José van Beckhoven Arjen Speksnijder 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(4):477-485
For detection of the plant pathogenic Tulip virus X (TuVX), a panel of six recombinant antibodies was identified. To this end, a repertoire of variable domains from heavy-chain
immunoglobulins (VHH) was cloned from an alpaca, which had been immunized with TuVX. Binding domains were selected by phage
display and panning on immobilized TuVX particles. Recombinant VHH antibodies were tested for sensitivity in a sandwich ELISA,
and were demonstrated to be readily able to distinguish TuVX-infected tulip leaf material from uninfected leafs. No cross-reactivity
of the VHH antibodies to related flexiviridae was observed. Recombinant VHHs maintained their reactivity upon storage at −20°C for over a year. The effect of incubation
at higher temperatures for prolonged time was studied. Two out of three VHH proteins retained activity after several weeks
of storage at 37°C. 相似文献
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Cadar D Cságola A Spinu M Dán A Ursu K Lorincz M Tuboly T 《Acta veterinaria Hungarica》2010,58(4):475-481
Porcine circoviruses (PCV) are widespread in domestic pigs worldwide and there is growing information about the presence of PCV in other suid species. Based on serological studies with sera of wild boars, it was established that PCV1 was present in these animals and antibodies specific to PCV2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of PMWS. Studies including PCV2 genome or antigen detection confirmed the previous findings. This is the first report about the presence of PCV in Transylvanian wild boar populations. Four hundred and sixty-nine samples were collected and grouped according to geographic origin, tested for the presence of PCV DNA using a real-time quantitative polymerase chain reaction assay, and 13.52% of the animals proved to be positive for one or in three cases both of the PCV genotypes. PCV2 was detected in all of the PCV-positive samples. 相似文献
18.
Florent M. Kinkingninhoun-Mêdagbé Aliou Diagne Franklin Simtowe Afiavi R. Agboh-Noameshie Patrice Y. Adégbola 《Agriculture and Human Values》2010,27(1):57-69
This paper examines the occurrence and impact of gender discrimination in access to production resources on the income, productivity,
and technical efficiency of farmers. Through an empirical investigation of farmers from Koussin-Lélé, a semi-collective irrigated
rice scheme in central Benin, we find that female rice farmers are particularly discriminated against with regard to scheme
membership and access to land and equipment, resulting in significant negative impacts on their productivity and income. Although
women have lower productivity, they are as technically efficient as men. The findings suggest that there is considerable scope
for improving the productivity of women through increasing their access to production resources. 相似文献
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雏鸡需要最佳的育雏条件,以帮助其成功地渡过最佳生长期或育成期。荷兰生产的HatchBrood育雏器能提供这些条件,就像仍由抱窝母鸡照顾出壳后最初4 d中的雏鸡一样。然而,不同的是HatchBrood育雏器能够在一个育雏室内一次照顾39 600羽雏鸡。 相似文献