Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. 相似文献
Temporal depositional rates are important in order to understand the production and occurrence of perchlorate (ClO4−) as limited information exists regarding the impact of anthropogenic production or atmospheric pollution on ClO4− deposition. Perchlorate concentrations in discrete ice core samples from the Eclipse Icefield (Yukon Territory, Canada) and
Upper Fremont Glacier (Wyoming, USA) were analyzed using ion chromatography tandem mass spectrometry to evaluate temporal
changes in the deposition of ClO4− in North America. The ice core samples cover a time period from 1726 to 1993 and 1970 to 2002 for the Upper Fremont Glacier
(UFG) and Eclipse ice cores, respectively. The average ClO4− concentration in the Eclipse ice core for the time period from 1970 to 1973 was 0.6 ± 0.3 ng L−1, with higher values of 2.3 ± 1.7 and 2.2 ± 2.0 ng L−1 for the periods 1982–1986 and 1999–2002, respectively. All pre-1980 ice core samples from the UFG had ClO4− concentrations <0.2 ng L−1, and the post-1980 samples ranged from <0.2 ng L−1 to a maximum of 2.6 ng L−1 for the year 1992. A significant positive correlation (R = 0.75, N = 15, p < 0.001) of ClO4− with SO42− was found for the annual UFG ice core layers and of ClO4− with SO42− and NO3− in sub-annual Eclipse ice samples (R > 0.3, N = 121, p < 0.002). The estimated yearly ClO4− depositional flux for the Eclipse ice core ranged from 0.6 (1970) to 4.7 μg m−2 year−1 (1982) and the UFG from <0.1 (pre-1980) to 1.4 μg m−2 year−1 (1992). There was no consistent seasonal variation in the ClO4− depositional flux for the Eclipse ice core, in contrast to a previous study on the Arctic region. The presence of ClO4− in these ice cores might correspond to an intermittent source such as volcanic eruptions and/or any anthropogenic forcing
that may directly or indirectly aid in atmospheric ClO4− formation. 相似文献
Cotton bollworm, Helicoverpa armigera Hubner selected for five generations with Bacillus thuringiensis insecticidal crystal protein Cry1Ac in the laboratory developed 12.98-fold resistance. Resistance and susceptible populations
were mass crossed to study the dominance of resistance. The Cry1Ac—selected (BCR) population showed 5.8-fold resistance to
Cry1Aa and 5.04-fold resistance to Cry1Ab. The degree of dominance (D) was 0.34 and 0.40 for the R × S and S × R hybrids, respectively, which indicates incomplete recessive character of Cry1Ac resistance in the population. The estimated
realized heritability (h2) and response quotient (Q) of resistance for Cry1Ac were 0.52 and 0.15, respectively. This indicated the lower phenotypic variation in the selected
population. The resistance risk assessment based on h2 indicated that the resistance would increased tenfold after <9 generations for Cry1Ac in the resistant population. The results
show the ability of H. armigera to develop resistance against Cry1Ac and cross-resistance to Cry1Aa and Cry1Ab. 相似文献
New icetexane diterpenes (1-2); 8, 11, 13-icetexatriene-10-hydroxy, 11, 12, 16-tri acetoxyl (1) and 8, 11, 13-icetexatriene-7, 10, 11-dihydroxy-12, 13-dihydrofuran (2) along with six known compounds namely acetoxy syranzaldehyde (3), syranzaldehyde (4), coniferaldehyde (5), lupeol (6), betulin (7), and 4-(4-methoxy phenyl)-2-butanone (8) were isolated from the roots of Premna tomentosa. The structures of compounds 1 and 2 were established by detailed spectral analysis using UV, IR, 1H NMR, 13C NMR, 1D, 2D and Mass. The newly isolated compounds were screened for rat intestinal α-glucosidase inhibitory and free radical (DPPH) scavenging potentiality. The new icetexane diterpenes (1, 2) and compound 3 were found to have significant α-glucosidase inhibitory and also free radical scavenging (DPPH) activities. 相似文献
The present study is an attempt to prepare synthetic seeds using alginate encapsulation of nodal explants of Sterculia urens. Regeneration potential of encapsulated nodal segments was tested on MS basal, MS + BA (3.0 mg L?1), MS + TDZ (0.2 mg L?1), and MS + BA (0.5 mg L?1) + NAA (2.0 mg L?1). The regeneration potential was maximum (73.33 ± 1.33) on MS + TDZ (0.2 mg L?1) followed by (69.33 ± 1.76) on MS + BA (3 mg L?1) even after 6 months of storage at 4°C, whereas controls did not show any regeneration potential after 1 week. These findings suggest synthetic seed technology as an alternative method for micropropagation and germplasm conservation of Sterculia urens an endangered species. 相似文献
1. Wheat, triticale 131 or triticale 72‐S could replace up to half the maize in broiler diets without affecting performance. Replacing more of the maize on either an equal nitrogen basis or a weight basis adversely affected performance.
2. Triticale 72‐S appeared to be superior to triticale 131 as a direct replacement for maize, but both grains were inferior to maize as energy sources. 相似文献
1. The nitrogen‐corrected metabolisable energy contents of the triticale varieties Multi Graze, Graze Grain 70, 204 and 131 were 12.28, 12.69, 12.37 and 12.03 MJ/kg respectively.
2. Increasing proportions (0, 25, 50 or 75%) of the maize of a diet were replaced by triticale 131 to provide diets of equal energy and nitrogen contents for broilers. These diets resulted in weight gains of 1.775, 1.782, 1.713 and 1.808 kg respectively at 8 weeks and there were no significant differences among efficiencies of food conversions.
3. Dressed carcass weights as percentages of body weight were 74.38, 75.01, 73.98 and 74.50 respectively and did not differ significantly.
4. Triticale in the diet did not seem to influence the relative weights of either liver or gizzard.
5. The flavour of the meat seemed to become less acceptable as the triticale in the diet increased but there were no significant differences in tenderness and juiciness of the meat caused by the treatments. 相似文献
An experiment was carried out at the Agronomy Field Laboratory, Bangladesh Agricultural University (BAU), Mymensingh in Old
Brahmaputra Alluvial Flood Plain (AEZ-9) during December 2005 to May 2006 to examine the root growth of different rice cultivars
under various establishment methods. This experiment comprised two sets of treatment (i) three methods of planting viz., dry
direct seeding, wet direct seeding and transplanting, and (ii) six rice cultivars, two inbreds viz., BRRI Dhan 28 and BRRI
Dhan 29, two hybrids, viz., Aalok 6201 and Sonarbangla 1 and two local improved cultivar viz., Habigonj IV and Habigonj VIII.
Cultivars had more remarkable effect on different root parameters i.e. number of roots, root length, root length density,
root biomass, root weight ratio and root shoot ratio of hybrid, inbred and local improved rice cultivars than method of planting.
BRRI Dhan 28 performed the best for most of the parameters. At harvest, BRRI Dhan 29 showed the best rooting behavior among
the cultivars. For both stages hybrid rice cultivars showed intermediate and local improved cultivars performed lower in rooting
behavior. Direct seeding method performed better than transplanting method for most of the root parameters but wet direct
seeding and dry direct seeding methods were identical. Interaction effect between method of planting and cultivar in most
of the root parameters were insignificant. But, BRRI Dhan 28 and BRRI Dhan 29 under wet direct seeding method and Sonarbanngla
1 under transplanting method showed the best rooting performance among the other combinations. 相似文献