Compositional Differences in Anthocyanins from Blue‐ and Purple‐Grained Spring Wheat Grown in Four Environments in Central Saskatchewan

Anthocyanins are important dietary components that play significant roles in human health because of their antioxidant and anti‐inflammatory properties. In the present study nine anthocyanin‐pigmented spring wheat lines grown at two sites in central Saskatchewan, Canada, were evaluated in terms of anthocyanin composition in comparison with four wheat checks over a two‐year period. The genotypes studied were blue‐aleurone and purple‐pericarp wheat. The anthocyanin pigments were quantified and identified with liquid chromatography and mass spectrometry. Two anthocyanin profiles were identified in the breeding lines, namely, blue profile and purple profile. Wheat lines with a purple profile had overall higher anthocyanin concentrations and more pigments than the blue‐aleurone lines. The purple profile was also characterized by the presence of acylated anthocyanins containing malonyl and succinyl substituents, but no acylated pigments were found in the blue profile. Delphinidin was the dominant aglycone in the blue profile, whereas cyanidin was the principal anthocyanidin in the purple profile. Genotype and interactions among genotype, year, and location were found to significantly influence content and composition of anthocyanin pigments. These differences in anthocyanin content and composition reflect various color characteristics and bioactivity for purple or blue wheat when utilized.     

Environmental Impacts of Heavy Metal Discharges from a Smelter in Deûle-canal Sediments (Northern France): Concentration Levels and Chemical Fractionation

Heavy metal pollution in sediments derived from the Deûle canal and sampled at different sites not far from a smelting plant has been examined in the present work in order to identify the sources of these metals and to assess the sediment environmental quality. The total concentrations of lead, zinc, cadmium, thallium, indium and tin in the samples were determined using inductively coupled plasma-atomic emission spectroscopy (ICP-AES). Our investigations have revealed that metal pollution is readily apparent in the studied sediments, with metals contents largely exceeding those measured in the background soils: maximum values are obtained for sediments collected near the industrial zone. The chemical forms of Pb, Zn, Cd, Tl, In and Sn in these sediments have also been studied using a sequential extraction method in order to evaluate their possible mobility, bioavailability and toxicity in this aquatic environment. Overall, the averaged fractionation of Pb and Zn is dominated, in a decreasing order, by the easily reducible, oxidizable and carbonate fractions. The importance of oxidizable phase (which is assumed to be composed mainly of organic matter and sulphides) in the Pb and Zn fractionations has been confirmed by the detection of X-ray diffraction peaks ascribed to galena (PbS) and wurtzite (ZnS) in contaminated sediment samples. Anthropogenic Tl, In, and Cd are mainly retained in Fe–Mn oxides/hydroxides, whereas anthropogenic Sn predominates in aluminosilicates/clays. We suspect that elevated percentage levels of Pb, Zn, Cd and In in the reducible fraction constitute a particular potential risk to this aquatic environment in case early diagenetic phenomena (that are observed in the sedimentary material) and physical disturbances (that occur in the water column) both take place strongly in the medium.     

Alzheimer's disease: current status of etiopathogenesis and therapeutic strategies

Alzheimer's Disease (AD) is one of the most common age-related neurodegenerative diseases. It is the most prevalent form of dementia, a general term for memory loss. It is characterized by progressive cognitive dysfunction, various behavioral and neuro-psychiatric disturbances that seriously interfere with daily life. Scientists have identified factors that appear to play a role in the development of AD but no definitive causes have been found for this complex disorder. The pathogenesis of Alzheimer's disease is highly complex. While several pathologies characterize this disease, amyloid plaques and neurofibrillary tangles are hallmark neuropathological lesions in AD brain. Current AD therapies are merely palliative and only temporarily slow cognitive decline and treatments that address the underlying pathologic mechanisms of AD are still lacking. In this review, we focus on the current aspects of AD ranging from the key risk factors for AD, the underlying pathogenic events and the novel medications including disease-modifying properties.     

Biofumigation with Fresh Manure or Brassicaceae Residuals could be a Promising Methyl Bromide Alternative in Head Lettuce Production

The current study investigates the natural alternatives of methyl bromide on head lettuce plants grown in greenhouse. Lettuce (Lactuca sativa L.) seedlings (cv. “Big Bell”) were treated with six different MBr alternatives which are, Biofumigation with fresh cow manure, fresh chicken manure, or Brassicaceae residuals plus Bio-Compost application (using disease suppressive compost) in comparison with chemical control with one of MBr chemical alternatives (Basamid) and finally negative control (which is the standard farmer treatment). Despite that chemical control with Basamid recorded the highest survival rate, biofumigation with fresh chicken manure gave the best head length, diameter, fresh weight, dry weight, total and marketable yield. Also, highest head quality characteristics such as TSS, SPAD reading, and nitrogen content were recorded with biofumigation with fresh chicken manure. Regardless of the superiority of Biofumigation with fresh chicken manure, Biofumigation with fresh cow manure or Brassicaceae residuals showed a better vegetative growth and quality parameters than farmer treatment (control), and they had significantly less effect than chemical control treatment. Bio-Compost fortified with Trichoderma harzianum and Bacillus subtilis (disease suppressive compost) recorded survival rate similar to biofumigation treatments but were significantly lower than chemical control treatment. Bio-Compost treatment did not result in a good result regarding total and marketable yield head fresh and dry weight. There was no detected significant effect of the studied treatments on stem diameter, L?ascorbic acid (vitamin C), phosphorus and potassium content     

Cyclodepsipeptides: Isolation from Endophytic Fungi of Sarcophyton ehrenbergi and Verification of Their Larvicidal Activity via In-Vitro and In-Silico Studies

Culex pipiens mosquitoes are vectors to many viruses and can transmit diseases such as filariasis and avian malaria. The present study evaluated the larvicidal activity of marine-derived endophytic fungi Aspergillus nomius and Aspergillus flavus from the soft coral Sarcophyton ehrenbergi along with two known cyclodepsipeptide compounds, scopularide A (1) and B (2), isolated from A. flavus extract, against third-instar larvae of C. pipiens, using distilled water as a negative control and toosenedanin as a positive control. The structures of the isolated compounds were confirmed by various spectroscopic analyses. The lethal concentrations (LC₅₀ and LC₉₀) were calculated by probit analysis. Scopularide A was the most potent after 96 h treatment, with LC₅₀ and LC₉₀ values of 58.96 and 994.31 ppm, respectively, and with 82.66% mortality at a concentration of 300 ppm. To unravel the biochemical mechanism of the tested extracts and compounds, their effects against protease, chitinase, phenoloxidases and lipase enzymes from the whole-body tissue of C. pipiens were evaluated after 72 h treatment at LC₅₀ dose. Superior activity was observed for A. flavus extract against all tested enzymes. A molecular docking study was conducted for scopularide A and B on the four tested enzymes, to further verify the observed activity. Results revealed good binding affinities for both compounds as compared to the docked ligands, mainly via a number of hydrogen bonds. This was the first study to report the isolation of endophytic fungi A. flavus and A. nomius from the marine soft coral S. ehrenbergi. The endophytic fungal extract of A. flavus was found to be a promising source for a natural larvicidal agent against C. pipiens populations.