Alternate day supplementation of corn stalk diets with soybean meal or corn gluten meal fed to ruminants.

Four experiments were conducted to determine the effect of adding corn gluten mean (CGM) or soybean meal (SBM) at 24- or 48-h intervals to diets based on corn stalks. In each experiment corn stalks was the primary diet ingredient fed to wethers or steers. Monensin was also fed to determine whether its effects on ruminal fermentation would improve the efficiency of N utilization under these conditions. Evaluation criteria included ruminal fermentation characteristics, DM intake and utilization, N balance in sheep, and steer feedlot performance. Ruminal ammonia nitrogen (NH3 N) concentrations measured over time were higher (P < .05) when diets contained SBM. Diet did not influence (P > .10) total VFA concentrations in ruminal fluid. Differences in diurnal shifts in ruminal NH3 N and total VFA due to protein source resulted in diet x hour interactions (P < .05). Dry matter intake response to protein source and frequency of supplement feeding was variable. Dry matter digestibility and nitrogen digestibility were not affected (P > .10) by protein source or feeding interval. The 48-h interval feeding of CGM was favorable compared with 24-h interval feeding (P < .05). The opposite response occurred with SBM, resulting in a diet x feeding interval interaction (P < .05). Nitrogen retention was greater (P < .05) when CGM was fed and with alternate day feeding. Diets that contained CGM supported higher (P < .05) ADG and gain/feed than diets that contained SBM when fed to steer calves. Alternate day feeding of supplements that contained monensin was detrimental to steer performance under the conditions of these experiments. Corn gluten meal is an effective substitute for SBM when alternate day protein supplementation is practiced.     

Problems associated with the serological diagnosis of Leptospira interrogans serovar hardjo infection in bovine populations

Data combining sequential bacteriology and serology from a longitudinal study of a dairy herd were used to demonstrate the limitations of serology as a diagnostic method in cross-sectional sampling of bovine populations. Whole-herd point serological prevalences showed considerable variation over a two-year sampling period (38.8 to 76.2 per cent), and this was mainly due to varying age-specific prevalence. Owing to the rapid decline in titres and the varying persistence of infection, point serological prevalences failed to approximate to cumulative infection rates (either past or present) at different times of the year. A higher estimate of the number of susceptible animals in the herd than is the case results in inaccurate information on true incidence rates and can confuse assessments of the susceptibility of different age groups, especially if only small numbers are sampled. A sampling exercise demonstrated that a 10-cow sample usually provided little useful information other than establishing the presence or absence of hardjo in the herd. Increasing the sample size markedly improved epidemiological information, investigations of clinical disease, assessments of vaccination needs and public health tracebacks. Preferably 10 sera from each of the yearling, first calver, second calver and older age groups should be tested. Serology was an inadequate indicator of infection in individual animals. Group geometric mean titres taken from a mean serological response curve were shown to have limited application in the interpretation of field data, unless infection had occurred in the previous two months.     

IMAGING DIAGNOSIS — MAGNETIC RESONANCE IMAGING OF INTRACRANIAL INFLAMMATORY FIBROSARCOMA IN A MIXED BREED DOG

An 8‐year‐old mixed‐breed dog presented with progressive behavioral changes and altered mentation. Magnetic resonance imaging (MRI) of the brain revealed an olfactory and frontal lobe extra‐axial mass. The mass exhibited the following MRI signal intensity characteristics: T2W mixed, T1W iso‐ to hypointense, FLAIR hyperintense, and strong contrast enhancement. The mass was removed with cavitronic ultrasonic surgical aspirator (CUSA) assisted neurosurgery. Based on histopathological appearance and immunohistochemistry, the diagnosis of inflammatory fibrosarcoma was made. To our knowledge, this is the first report describing MRI characteristics of intracranial inflammatory fibrosarcoma in the veterinary literature.     

Listeria monocytogenes septicemia in an immunocompromised dog

An 11‐year‐old, male castrated, Boston Terrier was presented to the North Carolina State University College of Veterinary Medicine Small Animal Emergency Service with a 2‐day history of progressive ataxia, left‐sided head tilt, and anorexia. The dog had previously been diagnosed with chronic lymphoid leukemia and suspected immune‐mediated destruction of his bone marrow precursor cells, possibly due to therapy with immunosuppressive dosages of prednisone and azathioprine. During the physical examination, abnormal findings included an increased body temperature and horizontal nystagmus. Diagnostic investigations included a computed tomography (CT) scan, which confirmed bilateral otitis media, and a blood culture, which was positive for Listeria monocytogenes serotype 4b (epidemic clone 1). Upon treatment with ampicillin/sulbactam, enrofloxacin, and minocycline, the dog became normothermic and the neurologic signs improved. L monocytogenes serotype 4b (epidemic clone 1) has been associated with outbreaks of human listeriosis originating from food contamination. Although rare case reports of Listeria spp. infection in dogs exist, an actual infection with the epidemic clone 1 strain has never before been reported in a dog. It should be included in the differential diagnoses in immunocompromised dogs with clinical signs of septicemia.     

Imaging the Mott insulator shells by using atomic clock shifts

Microwave spectroscopy was used to probe the superfluid-Mott insulator transition of a Bose-Einstein condensate in a three-dimensional optical lattice. By using density-dependent transition frequency shifts, we were able to spectroscopically distinguish sites with different occupation numbers and to directly image sites with occupation numbers from one to five, revealing the shell structure of the Mott insulator phase. We used this spectroscopy to determine the onsite interaction and lifetime for individual shells.     

Effects of opening size and stand characteristics on light transmittance and temperature under young trembling aspen stands

While aspen (Populus tremuloides Michx.) cover provides protection from growing season radiative frosts, reduced light levels can limit growth of understory white spruce (Picea glauca (Moench) Voss). The creation of openings around individual spruce can provide more light, but the chance of radiative frost damage increases as gaps become larger. We examined the relationship between opening size and light and temperature levels in the center of circular gaps created in young aspen stands ranging from 1.8 to 8 m tall. Models for predicting transmittance as a function of sky view factor and transmittance under intact stands are presented. Results indicated a strong relationship between light levels and opening size after considering pre-treatment light levels. The relationship between opening size and temperature was weak with minimum temperatures being more affected by the height and density of the surrounding stand than opening size.     

Indigenous Agroforestry Systems in Amazonia: From Prehistory to Today

Understanding the historical development of indigenous systems will provide valuable information for the design of ecologically desirable agroforestry production systems. Such studies have been relatively few, especially in Amazonia. The agroforestry systems in Amazonia follow a trail that begins with the arrival of the first hunter-gatherers in prehistoric times, followed by the domestication of plants for agriculture, the development of complex societies rich in material culture, the decimation of these societies by European diseases, warfare, and slavery, the introduction of exotic species, and finally, the present-day scenario of widespread deforestation, in which agroforestry is ascribed a potential role as an alternative land use. Despite the upheavals which occurred in colonial times, greatly reducing the population of native tribes, a review of anthropological and ethnobiological literature from recent decades indicates that a great variety of indigenous agroforestry practices still exist, ranging from deliberate planting of trees in homegardens and fields to the management of volunteer seedlings of both cultivated and wild species. These practices result in various configurations of agroforestry systems, such as homegardens, tree/crop combinations in fields, orchards of mixed fruit trees, and enriched fallows. Together they constitute a stock of knowledge developed over millenia, and represent technologies that evolved along with the domestication of native forest species and their incorporation into food production systems. This knowledge is the basis for the principal agroforestry practice employed by farmers in Amazonia today, the homegarden, and has potential to contribute to the development of other agroforestry systems.