A fusion DNA vaccine co-expressed Eimeria tenella TA4 and chicken IL-2 (chIL-2) was constructed and its efficacy against E. tenella challenge was observed. TA4 gene of E. tenella and chIL-2 gene were cloned into expression vector pcDNA3.1 and pcDNA4.0c in different forms, producing vaccines pcDNA3.1-TA4-IL-2, pcDNA3.1-TA4 and pcDNA4.0c-IL-2. The expression of aim genes in vivo was detected by RT-PCR and western blot. Animal experiment was carried out to evaluate the immune efficacy of the vaccines. Results indicated these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA3.0-TA4-IL-2 group was 192, higher than that of pcDNA3.1-TA4 group. The results suggested that TA4 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance DNA vaccine immunity. 相似文献
Studies of biodiversity dynamics have been cast on either long(systematics)or short(ecology)time scales,leaving a gap in coverage for moderate time scales of de... 相似文献
The aim of the present work was to investigate the effect of Rhus coriaria L. inclusion to the diet on some biochemical, haematological parameters and the level of antioxidant status of male rabbits. Adult rabbits were divided into five groups: one control (C) and four experimental groups. Experimental animals received sumac per os in feed in various doses (0.50%, 0.75%, 1.00% and 1.50%) for 90 days. Significant increase in PDWc (platelet distribution width) in E3 group when compared with control group was recorded. Sumac administration resulted in decreased cholesterol levels in all experimental groups vs. control group. Significantly lower level of cholesterol was found in E4 group with highest dose of sumac (1.50%). Higher values of total antioxidant status (TAS) and albumins were observed in all experimental groups in comparison with control group. A significant increase in TAS was detected in group E1 and E4. Concentrations of albumins were significantly higher in groups E3 and E4 vs. control group. Sumac administration had no significant effect on bilirubin content. In conclusion, these results show a positive effect of sumac consumption on antioxidant status and cholesterol level in adult male rabbits. 相似文献
ObjectivesTo study the effect of pregnancy and lactation on echocardiographic parameters in Holstein dairy cows.AnimalsNine multiparous high milk producing (HMP) dairy cows (producing more than 40 kg of milk per day in peak production) and 9 low milk producing (LMP) cows (producing less than 30 kg or milk per day in peak production).MethodsEchocardiography was performed twice; one month before calving and two months after calving.ResultsThe heart rate of HMP cows in the early lactation period was significantly higher than in the dry period. In LMP cows, there was a significant increase in left ventricular dimension in the early lactation period as compared to the dry period, and the interventricular septum in systole (IVSs) in the dry period was significantly thicker than early lactation period. In HMP cows, there was an increase in the right ventricular diameter in systole in the early lactation period as compared to the dry period. Left ventricular and aortic dimensions in the dry period of HMP were significantly higher than those of LMP cows. When the data were corrected for body weight, comparison of values of the dry period of HMP and LMP cows showed that left ventricular volume in systole in HMP was significantly higher and that IVSs, left ventricular fractional shortening and ejection fraction were significantly lower than in LMP cows.ConclusionsThis study demonstrated that lactation influences the intracardiac dimensions. The amount of milk production can influence echocardiographic parameters in dairy cows. 相似文献
The aim of this study was to evaluate the water and nitrogen use efficiency and some quantitative and qualitative characteristics of forage beet cultivars under the influence of different irrigation methods and nitrogen levels in two cropping years, 2017–18 and 2018–19, at Agricultural Research Station in Karaj, Iran. Experimental factors included the first factor with four irrigation methods (normal leakage, alternate furrow irrigation, fixed furrow irrigation, type (drip-strip)), the second factor was the amount of nitrogen fertilizer with three levels (150, 200 and 250?kg N ha?1) and the third factor included three forage beet cultivars (Sbsi052, Jamon and Kyros). Among irrigation treatments, alternate furrow irrigation and fixed furrow irrigation had the highest sugar content with 9.28% and 9.17%, respectively. The highest yield of digestible organic matter was obtained in leakage irrigation treatment, nitrogen fertilizer of 250?kg ha?1 and in Kyros at the rate of 19.45?t ha?1. The highest yield of root digestible dry matter, potassium, sodium and free nitrogen was observed in leakage irrigation treatment and consumption of 200?kg ha?1 nitrogen was observed in foreign cultivars. The highest crude protein was observed in alternate furrow irrigation conditions with a consumption of 200?kg ha?1 nitrogen in cultivar Sbsi052 at 13.08%. Leakage irrigation and type tape had the highest consumption efficiency and efficiency of nitrogen uptake with application of 150 and 200?kg ha?1 N, and the highest water use efficiency was also observed in leakage irrigation and type tape with application of 250?kg ha?1 N in domestic and foreign cultivars. The type irrigation method showed better quantitative and qualitative yield than the furrow irrigation methods.
Journal of Plant Diseases and Protection - In the original publication, the article title was incorrectly published as ‘Impact of leave infestation by herbivorous insects on the elemental... 相似文献
The effects of maternal salinity and light incubation on the salinity tolerance of the facultative halophyte Anabasis setifera during their germination stages were assessed. Seeds were collected from non-saline habitats in Egypt and saline habitats in the United Arab Emirates(UAE). The seeds of the two populations were germinated in 0, 100, 200, 400, 600 and 800 m M Na Cl, and incubated at 25°C/15°C in both 12-h light and 12-h darkness regimes and continuous darkness. Significantly more seeds germinated in the Egyptian population than in the UAE population. Salinity tolerance was significantly greater with the Egyptian population than with the UAE population, especially under the conditions of higher salinities. The difference in salinity tolerance between the seeds of two populations was attributed to their seed mass. In addition, germination was significantly faster for the Egyptian population than for the UAE population. Most of the saline treated seeds were able to recover their germination when transferred to distilled water, but this depended on their maternal salinity and light incubation. Recovery from higher salinities was significantly better for the seeds under darkness than for those under light in the UAE population, but the reverse was true for the seeds in the Egyptian population. The higher salinity tolerance for the A. setifera seeds from the non-saline Egyptian population and the lower salinity tolerance for the seeds from the saline UAE population cannot explain their natural distribution. Further studies about other possible roles, such as levels of different promoting and inhibiting phytohormones, are needed to understand the importance of salinity as an environmentally induced maternal effect. 相似文献