Changes in plasma biochemistry in horses competing in a 160 km endurance ride

SUMMARY Plasma biochemical values were measured in 14 horses, before a 160 km endurance ride, immediately after 85 km, immediately after 160 km, after 30 min recovery period and the day after the ride. For statistical analysis, a group of 7 horses that completed the ride at a mean speed of 234 metres per min (m/min) (Fast Group) was compared with 7 horses that completed the ride at a mean speed of 144 m/min (Slow Group). Estimations were made of sodium, potassium, chloride, bicarbonate, glucose, creatinine, urea, bilirubin, iron, total protein, albumin, calcium, phosphate, cholesterol, alkaline phosphate, gammaglutamyl transferase, lactate dehydrogenase, a hydroxybutyrate dehydrogenase, asparate amino transferase, alanine amino transferase, Cortisol and insulin. Disturbances in boichemical values associated with endurance exercise reflected dehydration, hepatic dysfunction, reduced renal function and muscle damage. The faster the speed of exercise, the greater was the stress as reflected by Cortisol values. The Fast Group of horses also demonstrated lower glucose and higher CK and LD values than the Stow Group of horses. However, biochemical values the day after the ride were similar to values before the ride.     

Ultrastructure of the hydrophobic gastric surfactant barrier in the dog

OBJECTIVES: To confirm the hydrophobicity of the luminal surface of the canine stomach and to elucidate the ultrastructure of the lining imparting that property. DESIGN AND PROCEDURES: Gastric and duodenal mucosal samples from eight dogs were collected immediately after euthanasia and subjected to contact angle measurement using a goniometer. Other samples were examined by electron microscopy following a fixation procedure known to preserve phospholipids and oligolamellar structures. RESULTS: Contact angles for the canine gastric mucosal surface (85.1 +/- 5.5) were significantly greater (P < 0.0001) than for the duodenum (24.0 +/- 1.7). Electron microscopy revealed the existence of surfactant as abundant osmiophilic phospholipid material within the gastric and duodenal mucosae. CONCLUSION: We have confirmed the hydrophobic nature of the canine gastric mucosa whereas the luminal surface of the duodenum is hydrophilic. We propose that the water-repellent nature of the canine gastric lining contributes to the 'gastric mucosal barrier' and is imparted by an oligolamellar layer of surface-active phospholipid ('gastric surfactant') adsorbed to the surface. Both gastric and duodenal mucosae may also utilise phospholipids as an intercellular defense mechanism in the event that tight junctions are breached by acid. It is tempting to speculate that a deficiency of gastric phospholipids may predispose dogs to ulcers. Further, exogenous administration of phospholipids may be useful in preventing gastric ulceration.     

Variations in the surface characteristics of the sward and the short-term rate of herbage intake by calves and lambs

The results are reported of an examination of detailed measurements on the ingestive behaviour of calves and lambs in response to variations in the surface characteristics of swards under strip-grazing and continuous stocking managements. It is shown that intake per bite and the short-term rate of herbage intake were both sensitive to the height of the surface horizon above ground level but, at least under strip-grazing management, not to variations in herbage density in the grazed horizon. The rate of biting was less sensitive to variations in sward conditions, particularly under strip-grazing.
Both intake per bite and rate of intake were more sensitive to variations in grazing height under strip-grazing, where sward changes were rapid, than under continuous stocking, where they were slow. Under continuous stocking, ingestive behaviour was more sensitive to changes in sward conditions in lambs than in calves.     

The long-term effects of a range of pasture treatments applied to three semi-natural hill grassland communities. 1. Pasture production and botanical composition

Five pasture treatments: (1) controlled grazing, (2) controlled grazing + lime, (3) controlled grazing + lime + phosphate, (4) controlled grazing + lime + phosphate + oversown white clover and (5) controlled grazing + lime + phosphate + oversown white clover + oversown perennial ryegrass were applied to three semi-natural hill grassland communities. The communities were those dominated by Agrostis/Festuca, Molinia and Nardus. The proportion of Nardus at the Nardus-dominant site was substantially reduced by herbicide before treatments were applied. All treatments were grazed simultaneously by mature wether sheep on three occasions each year. There were two grazing periods each of 4 weeks duration between mid-May and mid-August with a further grazing period of 3 weeks starting mid-October. During each grazing period stock numbers were set so that a residual herbage mass of 560 kg DM ha ^?1 remained at the end of the grazing period. Measurements of net herbage accumulation (NHA) were made annually over a period of 13 years at each site. The green:dead ratio of grasses, species composition of the pasture and the pH of the soil were measured at intervals during the experiment. Estimates of mean annual NHA ranged from 3860 kg DM ha^?1 for treatment 1 (controlled grazing) to 5170 kg DM ha^?1 for treatment 5 (oversown white clover and perennial ryegrass). The application of lime and phosphate increased annual NHA by 300–350 kg DM ha^?1 with a further increase of around 400 kg DM ha^?1 when white clover was sown. Increases in NHA between year 1 and year 13 ranged from 30% for treatment 1 to around 55% for treatment 5. Although there was no difference in the mean NHA between sites, the herbage from the Agrostis/Festuca site contained a higher proportion of green grass and white clover than that from the other sites. The highest levels of green grass and white clover were found on the oversown treatments at each site. The grazing pressure exerted produced relatively little change in the botanical composition at the Agrostis/Festuca site. At the Molinia-dominant site the Molinia was largely replaced by Nardus during the first 6 years and Nardus also increased in cover at the Nardus site. Application of lime and phosphate generally increased the proportion of Poa pratensis, Festuca rubra and Agrotis tenuis but did not halt the spread of Nardus at the Molinia and Nardus sites. White clover and perennial ryegrass were successfully introduced by oversowing and proportions remained high throughout the 13 years. The cover by bryophyte spp. increased at all sites with the greatest increases occurring in each case on the less comprehensive pasture treatments. These results suggest that on Agrostis/Festuca-dominant pastures herbage biomass production can be increased with relatively low-cost pasture treatments while maintaining Species diversity. However, Nardus and Molinia dominant pastures are likely to require more comprehensive pasture treatments involving sown grasses and white clover to provide herbage of acceptable quality and avoid an increase in Nardus and bryophytes in the sward. With a regime of episodic summer grazing and the addition of fertilizers oversown pastures can be maintained over long periods of time.     

Equine neonatal septicaemia: 24 cases

Equine neonatal septicaemia was confirmed in 24 foals hospitalised at the Rural Veterinary Centre between 1989 and 1992 with suspected septicaemia. Septicaemia was confirmed by culture of bacteria from blood of live foals and tissues obtained at necropsy of foals that died or were euthanased. Pathogenic bacteria isolated were predominantly Enterobacteriaceae (including Escherichia coli and Salmonella serovars) and Actinobacillus equuli. Clinical manifestations of septicaemia included signs of depression, dehydration, abnormalities in body temperature and manifestations of localised infection including diarrhoea, pneumonia, and septic arthritis. Most common haemato-logical abnormalities were neutropenia and increase of circulating band neutrophils. Survival rate of foals with confirmed septicaemia was 70.8%. Survival was found to be less likely in the presence of pneumonia, severe signs of depression, marked haematological changes or septic arthritis at the time of admission. Seven foals were confirmed to have septic arthritis without concurrent septicaemia. Of these, 4 had multiple joint involvement. Bacteria isolated from infected joints were predominantly Salmonella serovars. Four foals with septic arthritis failed to survive, due to multiple joint infection, which was unresponsive to treatment. The clinical and haematological abnormalities present in foals with confirmed septicaemia and septic arthritis were consistent with those observed in other studies. The bacterial isolates from foals with confirmed septicaemia were similar to those isolated in other studies. In contrast, the bacteria isolated from foals with septic arthritis without concurrent septicaemia were different from other studies.     

The influence of sward condition on rates of herbage growth and senescence in mixed swards under continuous stocking management

A study was conducted to determine the influence of herbage mass under continuous stocking management with sheep on rates of herbage growth (G), senescence (S) and net production of green herbage (NP) in a mixed-species sward of Lolium perenne. Poa annua and Trifolium repens. Plots were maintained in as nearly constant herbage mass as possible at 500, 700, 1000 and 1700 kg organic matter (OM) ha⁻¹ by continuous but variable stocking with sheep for the period May to July inclusive. Estimates of G, S and NP were obtained for each species from repeated measurements over two 2-week periods on individually identified grass tillers or clover stolons. Estimates of species population densities enabled estimates of G. S and NP per unit area to be made.
Rates of G were higher and of S were lower for Lolium tillers and Trifolium stolons than for Poa tillers. The laminae of Poa tillers occupied inferior positions in the canopy of the swards maintained at higher herbage masses and were less accessible to the grazing animals, so that Poa tissue was less well utilized.
The total population densities of tillers and stolons declined at herbage masses both above and below 700 kg OM ha⁻¹. The rate of G for the three species combined increased with herbage     

Effect of oestrous synchrony between embryo donors and recipients,embryo quality and state on the pregnancy rate in beef cattle

This study aimed to evaluate the effect of oestrous synchrony between donors and recipients and the embryo quality on the pregnancy rate in beef cow recipients. The experiment was performed over two years at an embryo transfer (ET ) centre in Southern Brazil. Ninety Aberdeen Angus cows were subjected to superovulation (SOV ) protocols, resulting in the recovery of 1,048 transferable embryos. Eleven groups were formed with intervals of 6 hr, from ?30 to +30 hr, with respect to recipient versus donor oestrous detection. Evaluation of embryo quality was according to the IETS guidelines. The overall pregnancy rate was 52%. Effects related to donor and recipient oestrous synchronization on pregnancy rate were observed (p  =  .01), ranging from 36% to 50%. The embryo quality rate affected the pregnancy rate, where Grade I resulted in 57% and Grade III in 43% of pregnancy (p  <  .001). The embryonic state (frozen or fresh) showed no (p  >  .05) effect on pregnancy rate: 53% for fresh embryos and 44% for frozen embryos. The odds ratio for explanatory variables causing pregnancy indicated that Grade III embryos had 31% less chance of conception compared to Grade I. Thus, oestrous synchrony between donor and recipient, considering ±30 hr apart, can affect the pregnancy rate along with embryo quality.     

Herbage intake and milk production by grazing dairy cows 1. The effects of variation in herbage mass and daily herbage allowance in a short-term trial

Twenty-four British Friesian cows were allocated between six grazing treatments (two levels of herbage mass x three levels of daily herbage allowance) in a balanced change-over design with four periods of 12 d each at monthly intervals. Herbage OM mass ranged from 3790 to 5770 kg ha^-1 measured to ground level and daily herbage DM allowances were 30, 60 and 90 g per kg animal live weight. Herbage OM intake was lower at high than at low herbage mass (24·6 vs 26·3 g per kg LW), and was 26·9, 26·6 and 22·9 g per kg LW respectively at daily herbage DM allowances of 90, 60 and 30 g per kg LW. Milk yield was not affected by herbage mass but was depressed at the low herbage allowance.