IMPURITY IN HALOTHANE ANESTHETIC

A halogenated butene has been isolated from commercially available halothane (Fluothane). Its concentration increases under the conditions in which halothane is commonly used in clinical work.     

A mathematical model of the aerobic deterioration of silage

A mathematical model of the aerobic deterioration of silage is described that includes the physical processes of gas and heat Rows, as well as an established model of microbial growth and the associated chemical changes. Yeasts and acetic acid bacteria are included in the growth model. The version of the model described relates to a cylinder of silage subject to forced aeration, a common experimental configuration. The model was very sensitive to variations in insulation thickness and yeast growth rate, and to initial pH, especially when maize was the forage. Great care is needed when selecting input data for use in the model to be sure that the physical and chemical properties are accurate. The model solution was sensitive to grid size, but with 361 cells, the maximum grid resolution that could be used on a 486 series PC, solutions were achieved with an acceptably small loss of accuracy. The model predicted the results of an experiment on silage deterioration quite accurately. However, the model also predicted that considerable spatial gradients of parameters, such as temperature, pH, and lactic acid, would occur. This indicates the need for care when taking samples or making temperature measurements in deterioration experiments. The model is potentially a useful tool in predicting the optimal time and position to take samples for analysis in a time-course experiment.     

Further studies on the effect of phosphate fertilizer and propyzamide on the growth of white clover slot-seeded into permanent pasture

Assessments were made of the clover yield and clover content between early June and late July 1984 of white clover in a permanent pasture which had been slot-seeded in spring 1982.
Past reserves of phosphate or application of propyzamide alone only slightly increased clover dry matter yield but together they increased it from 13 to 785 kg dry matter ha^-1 and gave 31% clover in the total herbage yield. Applications of P₂O₅ during the experiment, alone or together with past reserves of phosphate, Iased clover yield from 13 to 242 kg dry matter ha^-1 and the proportion of clover from 1 to 10%. However, highest yields (1282 kg) and clover content (37%) were attained when P₂O₅ and propyzamide were applied to treatments with past reserves of phosphate.
These results confirmed the trends evident during the second year and also indicated a potential role for grass-suppressants in increasing management options during the early years after slot-seeding.     

The influence of seed rate and pesticides on the establishment and growth of white clover slot-seeded into a permanent pasture

The establishment and growth of white clover (cv. Grasslands Huia) was investigated in two experiments with seeds sown in slots cut in old permanent pasture in early May 1982. In the first experiment, fully protected from pests and diseases, either 50 (low), 100 (medium) or 200 (high) seeds were sown evenly per metre length of slot; 100 seeds were also sown in a clumped pattern unevenly. In the second experiment, the effects of various combinations of molluscicide, insecticide and fungicide were investigated.
Nine weeks after sowing there were 27, 37, 34 and 62 seedlings m⁻¹ on the low, medium even, medium uneven and high seed-rate treatments, representing percentage establishments of 54, 37, 34 and 31 respectively. By September, however, there were no significant differences between the dry weights m^−l of any of the treatments. In the second experiment, damage by slugs occurred early in treatments without molluscicide and caused a mean reduction of 70% in seedling establishment. As a result significantly smaller dry weights m⁻¹ of clover were recorded in September in these than in other treatments. Failure to protect against insects resulted in smaller seedlings.
The results indicated that variations in seed rate within the range investigated were not critical but that failure to protect against pests decreased percentage establishment and subsequent growth.     

Slot-seeding investigations. 5 The effects of simulated delayed emergence and propyzamide on the spread and growth of white clover in permanent pasture

The effects of delayed emergence of white clover (cv. Grasslands Huia) seedlings, following slot-seeding during spring and early summer 1983, were simulated by sowing seeds at intervals into slots cut in turves of permanent grassland in soil-filled tanks or in the field. The resulting spread and growth of the species were assessed during the following 15–18 months. The effects of propyzamide, a grass-suppressing herbicide (at 0·2 kg ha^-1), were also investigated in the field.
A delay of 10 or 18 days in the sowing of seeds in turves in the tanks reduced clover dry weight yield from 23 to 11 during 1983 and from 118 to 96 g (0·12 m)^-2 during 1984. Spread of stolons from the slots was also greatly delayed and effects persisted for at least a year. Increased clover growth on the earliest sown treatment more than compensated for a slightly smaller yield of grass.
In the field, yields of clover from late summer 1983 until spring 1984 closely reflected the order of emergence. Afterwards, differences were less clear-cut but the cumulative yield of clover until August 1984 of the earliest-sown treatment was twice that of a treatment sown 20 d later. Effects of delayed sowing in summer 1983 were more pronounced on stolons than on foliage growth when measured in September 1984, with significant reductions in many stolon attributes even with 8 d delay in sowing. Application of propyzamide in autumn 1983 increased clover yield during 1984 from 1·7 to 3·0 and total herbage yield from 7·1 to 8·6 t ha^-1.
The results demonstrated some of the benefits of rapid seedling emergence and of the use of a grass-suppressing herbicide, but did not indicate any interaction between the two factors.     

美国堪萨斯州不同天气事件与土壤类型条件下作物产量预测

Computer simulation was used for predictive analysis of the effects of weather and soil type on crop yield in the U.S.crop insurance program.The Environmental Policy Integrated Climate (EPIC) model was modified to include hail weather events,which completed the modifications necessary to simulate the four most frequent causes of crop yield loss (hail,excessive wet,excessive cold,and excessive dry) associated with soil type in Kansas,USA.At the region level,per hectare yields were simulated for corn,wheat,soybean,and sorghum.We concluded that it was possible to predict crop yields through computer simulation with greater than 93% accuracy.The hail damage model test indicated EPIC could predict hail-soil-induced yield losses reasonably well (R² > 0.6).The investigation of soil type influence on dryland sorghum and wheat production indicated that Wymore silty clay loam soil and Kenoma silt loam produced the highest sorghum yields statistically;Kuma silt loam,Roxbury silt loam,Crete silty clay loam,and Woodson silt soils produced the second highest sorghum yields statistically;and Richfiled silt loam,Wells loam,and Canadian sandy loam produced the lowest sorghum yields.By contrast,wheat production showed less sensitivity to soil type variation.The less sensitive response of wheat yields to the soil type could be largely due to the unconsidered small-scale variability of soil features.     

Effect of intravenous lidocaine administration on laminar inflammation in the black walnut extract model of laminitis

Reasons for performing study: Laminitis is a serious complication of horses suffering from sepsis/endotoxaemia‐related events. Laminitis in horses and organ injury in human sepsis are both reported to involve inflammatory injury to the laminae/organs including early activation of endothelium and leucocytes leading to emigration of neutrophils into the tissue interstitium. In the black walnut extract (BWE) model, systemic inflammatory events coincide with marked increase in laminar mRNA concentrations of inflammatory genes including proinflammatory cytokines (i.e. IL‐1β, IL‐6), COX‐2, chemokines (i.e. IL‐8) and endothelial adhesion molecules (i.e. ICAM‐1 and E‐selectin). In models of human sepsis, i.v. lidocaine has been reported to decrease leucocyte and endothelial activation, and the expression of proinflammatory cytokines and chemokines. Objectives: To evaluate the effect of i.v. lidocaine therapy on the inflammatory processes documented to occur in the BWE model of laminitis. Methods: Twelve horses were administered BWE and treated immediately with either lidocaine (1.3 mg/kg bwt bolus, followed by 0.05 mg/kg bwt/min CRI, n = 6) or saline (n = 6) for 10 h. At 10 h post BWE administration, laminar samples were obtained under general anaesthesia for assessment of proinflammatory gene expression (using RT‐qPCR) and leucocyte emigration (via CD13 immunohistochemistry). At 0, 3 and 10 h post BWE administration, skin samples were obtained for assessment of leucocyte emigration (via calprotectin immunohistochemistry). Results: No significant differences between groups were noted for inflammatory gene mRNA concentrations (IL‐1β, IL‐6, IL‐8, COX‐2) or for number of leucocytes present within the laminar interstitium or skin dermis. Increased (P<0.05) laminar E‐selectin mRNA concentrations were present in the LD group (vs. SAL group). Conclusions: Continuous administration of i.v. lidocaine does not inhibit inflammatory events in either the laminae or skin in the horse administered black walnut extract. Potential relevance: This work questions the use of continuous i.v. administration of lidocaine as an effective anti‐inflammatory therapy for systemic inflammation.     

IMAGING DIAGNOSIS—SPINAL CORD CHONDROSARCOMA ASSOCIATED WITH SPIROCERCOSIS IN A DOG

A 7‐year‐old neutered female Boerboel cross was examined for progressive left pelvic limb lameness. There was no left patellar reflex but the remaining pelvic limb reflexes were hyperreflexic. Radiographically, there was a poorly mineralized opacity occupying the intervertebral foramen at L4–L5. On computed tomography images there was a hyperattenuating intramedullary lesion at L4–L5 that continued caudally, lateralized to the left and became extramedullary, terminating at L5–L6. In addition, well marginated, hyperattenuating lesions were noted at two muscular sites. The dog underwent euthanasia and a caudal esophageal mass was found at post mortem examination. The tumors in the spinal cord, the esophagus, and the skeletal muscles were diagnosed histologically as low‐grade chondrosarcoma undergoing endochondral ossification. Spirocerca lupi‐induced esophageal chondrosarcoma was believed to be the primary site from which the other, presumably metastatic, lesions originated.