Costing the ex situ conservation of plant genetic resources at AVRDC – The World Vegetable Center

AVRDC – The World Vegetable Center maintains the world’s largest international public collection of vegetable genetic resources at its headquarters in Taiwan. The ex situ conservation and dissemination of germplasm to researchers and breeders worldwide contributes to global food and nutrition security but also carries considerable costs. The objective of this study is to quantify these costs for the 12-month period from September 2011 to August 2012 using the Decision Support Tool developed by the International Food Policy Research Institute. The results show that the present value of capital assets is USD 1.99 million for the facilities and USD 0.48 million for the equipment. The total annual cost is USD 0.684 million, of which 74 % are labor costs. The average conservation and dissemination cost per accession is USD 10.08 per year. Seed regeneration, seed processing, characterization, and seed dissemination are the four most costly operations of the genebank. The storage itself only contributes 17 % of the cost. In comparison, the average cost per accession is USD 5.15 at ICARDA, USD 6.84 at CIMMYT, USD 8.62 at ICRISAT, USD 9.19 at IRRI, and USD 22.52 at CIAT (in 2012 US dollar values). High labor costs in Taiwan increase AVRDC’s average cost, but the fact that more vegetable species are self-pollinating and thus less labor intensive to regenerate than cross-pollinating species keeps the average costs in check. These results are important benchmarks for other genebanks.     

Fixed‐time post‐cervical artificial insemination in weaned sows following buserelin use combined with/without eCG

Fixed‐time post‐cervical artificial insemination (FTAI) drastically reduces labour requirements and increases the use of boars with higher genetic merit. This study evaluated the efficiency of eCG administration combined with/without the GnRH agonist buserelin for the induction and synchronization of ovulation in weaned sows submitted to FTAI. The sows were allocated into three groups. In the control group, the first artificial insemination was performed at the onset of oestrus and repeated every 24 hr. In the eCG+GnRH group, sows received 600 IU eCG at weaning and buserelin (10 μg) after 86–89 hr of eCG, and in the GnRH group, sows received only buserelin after 86–89 hr of weaning. The hormone‐treated sows received a single FTAI after 30–33 hr of buserelin application. All the sows were inseminated with homospermic doses (1.5 × 10⁹ sperm cells/50 ml). The interval between weaning and ovulation was shorter (p < .05) in the eCG+GnRH (133.3 hr) and GnRH (135.9 hr) groups than the control (141.5 hr) group. In the eCG+GnRH group, the sows ovulated earlier (p < .05) than those in the GnRH group (44.5 vs. 48.2 hr after buserelin administration). The reproductive performance of GnRH sows was not compromised when only sows exhibiting oestrus at the time of insemination were considered, but lower farrowing rate and smaller litter size were observed in eCG+GnRH sows. The reproductive performance of eCG+GnRH sows was primarily compromised because the insemination was performed outside the optimal time relative to ovulation; therefore, it is advisable to inseminate them before 116–122 hr after weaning.