Comparison of yeast and algal diets for bivalve molluscs

Four species of bivalve molluscs were fed diets consisting of varying proportions of the yeast Candida utilis and the diatom Thalassiosira pseudonana. Juvenile Argopecten irradians, Mercenaria mercenaria, and Mytilus edulis grew as fast or faster than controls when fed diets containing as much as 50% yeast. Growth of soft tissue in Crassostrea virginica, however, decreased with the amount of yeast in the diet. The relative food values of the different diets were not closely correlated with gross chemical composition or amino acid composition.     

Glugea heraldi n. sp. (Microsporida, Glugeidae) from the seahorse Hippocampus erectus Perry

Abstract. A new species of Microsporida, Glugea heraldi , is described from the seahorse Hippocampus erectus . Measurements of fixed spores and notes on the histopathology of parasitized tissue are given.     

The evaluation of piromidic acid as an antibiotic in fish: an in vitro and in vivo study

Abstract. The activity of piromidic acid (PA, 8-ethyl-5, 8-dihydro-5-oxo-2-prior lidinopyrido [2,3–d] pyrimidine-6-carboxylic acid) against bacteria in fish was evaluated in vitro and in vivo .
In vitro , PA was effective against a wide range of pathogenic bacteria stocked in this laboratory and freshly isolated from fish. PA was active against organisms showing multiple drug-resistance and showed no cross-resistance to chloramphenicol, tetracycline and sulfamonomethoxine.
In vivo , orally administered PA was effective against experimental infections with Aeromonas hydrophila and Vibrio spp. in goldfish and eels. The efficacy of PA against these infections was equal to or higher than that of chloramphenicol, and higher than that of tetracycline. Distribution of PA in the Ash was investigated by bioassay and autoradiography. In the bioassay, orally administered PA was easily absorbed and distributed in the blood and tissues of major organs within 1 h after the administration. Peak levels were attained 2 to 4 h post dosing, and no residue was detected in the blood and tissues 48 h post dosing. The results obtained from the autoradiographic investigation in goldfish were similar to those from the bioassay. Therapeutic levels of PA were well tolerated by fish when administered with feed.     

Cellular immune response in rainbow trout Salmo gairdneri Richardson to Yersinia ruckeri O-antigen monitored by the passive haemolytic plaque assay test

Abstract. The specificity and kinetics of the immune response of rainbow trout ( Salmo gairdneri ) to single injections of an O-antigen extracted from the bacterial pathogen Yersinia ruckeri , which causes enteric redmouth in fish, were investigated by the passive haemolytic plaque assay and serum antibody quantitation. Doses ranging from 5 ng to 500 mg in 10-fold increments were injected intraperitoneally into groups of trout held at 17 × 1°5°C. The occurrence of plaque forming cells (PFC) and humoral antibody was followed for 35 days after injection. Trout gave an immune response to doses of 500 ng and above. Seven days after injection no humoral antibody was detected, but PFC were found in the spleen. The maximum PFC numbers occurred 11 days after injection. On day 21, few PFC were found, whereas serum antibody titres were highest. The antibody from immunized trout showed little or no cross-reactions with sheep red blood cells passively labelled With antigens from other fish pathogens.     

Photoperiod induced off-season spawning of pink salmon (Oncorhynchus gorbuscha)

Four groups of pink salmon, which had been reared under artificial light, became sexually mature and produced viable gametes: 59 days prior to, and 19 – 32 days, 115 days and 220 days after their expected date of reaching sexual maturity. Altered times of sexual maturation were obtained by accelerating, leaving unchanged, or decelerating the rate of change of photoperiod which each group of fish would normally receive during its first year of life. All groups of fish were exposed to a normal rate of change in photoperiod during their final year of life. Mean fecundity was reduced from the 800–2000 ova observed in wild stocks, and ranged from 629 for the 59 day advanced fish, to 862 for the 115 day delayed fish. Egg mortality during the period from fertilization to eyeing was much greater in the three groups of fish subjected to accelerated or decelerated rates of change in photoperiod than in the fish subjected to the normal rate of change in photoperiod. Some of the progeny of the 220 day delayed fish, which were reared under artificial light with the normal rate of change in photoperiod set 220 days out-of-phase, became sexually mature 2 years after they had begun life as fertilized eggs.     

Effect of time of spraying of glyphosate on the vegetation of an upland deep peat soil

The vegetation of an upland deep peat soil consisting mainly of Festuca rubra, Holcus mollis, Molinia caerulea, Juncus acutiflorus and Potentilla erecta was sprayed with 1·5 kg a.i. per ha glyphosate applied to separate plots at monthly intervals from April to August. In July of the following year, April and May applications showed no effect on sward composition while June, July and August treatments resulted in increasing proportions of mosses and bare ground. August spraying gave almost complete control of the indigenous species with the exception of F. rubra and mosses. The results are discussed in relation to the establishment of improved pastures in upland areas by surface seeding.     

Effects of grazing by lambs in autumn on a red clover-perennial ryegrass sward

In 1976, 1977 and 1978 a red clover-perennial ryegrass sward was cut twice for silage, and in the autumn of 1976 and 1977 it was either grazed at low and high stocking rates, i.e. seventeen and thirty-four lambs per ha respectively, or was cut with a forage harvester. The effects of grazing on yield in the following year were examined. Herbage growth in the grazing period was slow and did not differ significantly between the treatments. The yield of silage dry matter taken in May and July was highest in ungrazed plots (9·8 and 8·1 t ha^-1 in 1977 and 1978 respectively) and lowest in plots stocked at the high rate (5·9 and 5·7 t ha^-1 in 1977 and 1978 respectively). The aftermath yield for grazing in 1977 was slightly but significantly greater on treatments grazed in the previous year compared with ungrazed treatments. Red clover content decreased markedly during grazing, the high stocking rate treatment containing 2·4% clover and the ungrazed treatment 57·3% clover. The high stocking rate treatment also had the lowest red clover content in the first silage cut. Red clover content in grazed plots increased to a level similar to that in ungrazed plots by the start of the grazing period in the subsequent harvest year. Animal performance was higher at the low than at the high stocking rate but herbage consumption per head did not differ significantly between the two grazing treatments. Possible reasons for the adverse effect of grazing on the red clover are defoliation and treading. It is concluded that such experiments can form the basis of an economic assessment of red clover and help the farmer decide whether or not he should integrate the crop into his system.     

Regrowth of ryegrass swards subject to different cutting regimes and stocking densities

A cutting experiment was carried out on a sward of S23 perennial ryegrass to study the effect of different defoliation regimes on regrowth and the factors affecting regrowth. The defoliation frequencies and intensities encompassed the range commonly found on continuously stocked pastures. The defoliation regimes produced swards of contrasting growth form with regrowth characteristics which differed in respect of senescence loss, fall in water-soluble carbohydrate concentration, residual leaf area index, photosynthesis per unit leaf area index and photosynthesis per unit area of land. Regrowth was most closely related to the latter. These results were used to interpret data for net canopy photosynthesis of continuously grazed pastures.