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排序方式: 共有204条查询结果,搜索用时 31 毫秒
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Barbara C. Rütgen Irene Flickinger Birgitt Wolfesberger Barbara Litschauer Andrea Fuchs‐Baumgartinger Sabine E. Hammer Armin Saalmüller Ilse Schwendenwein 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(1):172-178
A 7‐year‐old male Boxer with a 3.5‐year history of atopy and food hypersensitivity was presented with multiple poorly circumscribed nodules and maculae of the skin and tongue, and jaundiced mucosal membranes. Cytologic and histopathologic examination of the skin lesions revealed cutaneous epitheliotropic lymphoma. Cells were CD3+ and CD8+ in flow cytometry. The CBC showed a moderate leukocytosis with 16% atypical lymphocytes with irregularly cleaved nuclei. Flow cytometric phenotyping of peripheral blood showed an elevated proportion of the CD8+ T‐lymphocyte subpopulation, indicating a malignant population of T‐cell origin, and the electropherogram of the PCR antigen receptor rearrangement produced a monoclonal peak for TCRγ. Liver enzyme activities were markedly increased and abdominal ultrasound examination showed increased echogenicity of the liver and enlarged abdominal lymph nodes. Fine‐needle aspirates of the liver confirmed infiltration with lymphocytes exhibiting the same morphology as the cells detected in skin and peripheral blood. Treatment was induced with L‐asparaginase, lomustine, and prednisone. Partial clinical remission of the skin and tongue lesions was achieved within 10 days, and hematologic abnormalities resolved. Despite further treatment with L‐asparaginase and lomustine, the dog relapsed within one month and was euthanized. Presence of malignant lymphocytes in skin, peripheral blood, and liver indicate a rare variant of leukemic cutaneous T‐cell lymphoma, equivalent of Sézary syndrome in a dog. This case report describes the use of flow cytometry as a complementary tool for lymphocyte characterization of skin lesions for the first time. 相似文献
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Albert J Aliu E Anderhub H Antoranz P Armada A Asensio M Baixeras C Barrio JA Bartelt M Bartko H Bastieri D Bavikadi SR Bednarek W Berger K Bigongiari C Biland A Bisesi E Bock RK Bordas P Bosch-Ramon V Bretz T Britvitch I Camara M Carmona E Chilingarian A Ciprini S Coarasa JA Commichau S Contreras JL Cortina J Curtef V Danielyan V Dazzi F De Angelis A de Los Reyes R De Lotto B Domingo-Santamaría E Dorner D Doro M Errando M Fagiolini M Ferenc D Fernández E Firpo R Flix J Fonseca MV Font L 《Science (New York, N.Y.)》2006,312(5781):1771-1773
Microquasars are binary star systems with relativistic radio-emitting jets. They are potential sources of cosmic rays and can be used to elucidate the physics of relativistic jets. We report the detection of variable gamma-ray emission above 100 gigaelectron volts from the microquasar LS I 61 + 303. Six orbital cycles were recorded. Several detections occur at a similar orbital phase, which suggests that the emission is periodic. The strongest gamma-ray emission is not observed when the two stars are closest to one another, implying a strong orbital modulation of the emission or absorption processes. 相似文献
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Teeling H Fuchs BM Becher D Klockow C Gardebrecht A Bennke CM Kassabgy M Huang S Mann AJ Waldmann J Weber M Klindworth A Otto A Lange J Bernhardt J Reinsch C Hecker M Peplies J Bockelmann FD Callies U Gerdts G Wichels A Wiltshire KH Glöckner FO Schweder T Amann R 《Science (New York, N.Y.)》2012,336(6081):608-611
Phytoplankton blooms characterize temperate ocean margin zones in spring. We investigated the bacterioplankton response to a diatom bloom in the North Sea and observed a dynamic succession of populations at genus-level resolution. Taxonomically distinct expressions of carbohydrate-active enzymes (transporters; in particular, TonB-dependent transporters) and phosphate acquisition strategies were found, indicating that distinct populations of Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria are specialized for successive decomposition of algal-derived organic matter. Our results suggest that algal substrate availability provided a series of ecological niches in which specialized populations could bloom. This reveals how planktonic species, despite their seemingly homogeneous habitat, can evade extinction by direct competition. 相似文献
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Fort M Baldone V Fuchs L Giménez H Rojas M Breccia JD Oyhenart J 《Veterinary microbiology》2012,156(3-4):439-442
Brucella suis biovar 1 is the causative agent of brucellosis in several domestic and wild animals and it is a common agent of human brucellosis. European hares (Lepus europaeus) have been shown to be infected by B. suis biovar 1 and the transmission to other animals has been suggested. In this work, experimental rabbits (Cuniculus orictolagus) were infected with B. suis biovar 1 isolated from wild hares. Infected rabbits showed high serological response in 2 weeks after discharge and typical granulomatous lesions (2mm diameter) were found in liver, spleen and kidneys after 50 days. B. suis biovar 1 was cultured from the lesion of the organs mentioned above as well as from urine, placenta and fetuses. These data suggest that hares are a potential source for horizontal transmission of B. suis biovar 1 to other mammalians. 相似文献
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Deutz A Fuchs K Schuller W Nowotny N Auer H Aspöck H Stünzner D Kerbl U Klement C Köfer J 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(7-8):306-311
The aim of this study was to investigate the seroprevalences to zoonotic pathogens in hunters, to propose preventive measures and to obtain more information about the occurrence of zoonotic pathogens in local wild animal populations. From 146 male and 3 female hunters originating from the south-eastern Austrian federal states of Styria and Burgenland blood samples were taken and anamnestic data were obtained using a questionnaire. The serological investigations included the following viral, bacterial and parasitic zoonotic agents or zoonoses, respectively (antibody-seroprevalences in brackets): encephalomyocarditis virus (EMCV, 15%), Puumala-Hantavirus (10%), Newcastle Disease virus (NDV, 4%), borreliosis (IgG 42%, IgM 7%), brucellosis (1%), chlamydiosis (3%), ehrlichiosis (IgG 15%, IgM 3%), leptospirosis (10%), tularaemia (3%), Q fever (0%), Echinococcus multilocularis/E. granulosus (5%/11%), toxocariasis (17%). Out of a control group of 50 persons (urban population, no hunters) only one person was found to be seropositive for Toxocara canis and NDV and four for EMCV, all other results were negative in the control group. The high seroprevalences especially to Borrelia burgdorferi s.l., Ehrlichia spp., Leptospira interrogans, E. granulosus, E. multilocularis, encephalomyocarditis virus and Puumala virus demonstrate that hunters are particularly exposed to zoonotic pathogens. It should also be noted that one hunter was seropositive for Brucella abortus and five exhibited antibodies to Francisella tularensis. In these cases, as well as in the cases of the 15 seropositives for Leptospira interrogans, the suspected source of infection may--besides rodents--also include wild boars and brown hares. The infections with NDV and Chlamydophila psittaci may be traced back to contact with certain species of birds (potential risk: aviaries). For Hantaviruses, rodents are considered to be the main source of human infections. 相似文献
49.
The objective of this flow cytometric study was to examine plasma membrane integrity, mitochondrial membrane potential (MMP) and the degree of DNA fragmentation of cryopreserved bovine sperm immediately (0 h) and 3 h after thawing and to compare the results with each other and with the fertility of bulls. Cryopreserved spermatozoa from 4 consecutive ejaculates of 20 bulls were examined. Percentages of plasma membrane intact sperm (PMI) and sperm showing a high MMP (HMMP), respectively, were determined by the SYBR14/PI‐ and the JC‐1 assays. DNA fragmentation was analysed by the standard deviation of the DNA fragmentation index (SD‐DFI) and the percentage of sperm with a high degree of DNA fragmentation (%DFI) by using SCSATM. The mean non‐return rate on day 56 (NRR 56) ranged from 63.7% to 78.0% (mean ± SD: 71.8% ± 3.7%). Mean values for PMI and HMMP decreased from 37.4% ± 6.8% to 31.2% ± 6.1% and from 38.8% ± 7.1% to 23.8% ± 7.7% respectively. SD‐DFI increased from 56.9% ± 8.0% to 69.0% ± 12.9% and %DFI from 6.4% ± 2.5% to 12.4% ± 5.8%. The correlation between PMI 0 h and HMMP 0 h (r = 0.95; p < 0.0001) was higher (p < 0.05) than that between PMI 3 h and HMMP 3 h (r = 0.88; p < 0.0001). %DFI 0 h was neither related to PMI 0 h nor to HMMP 0 h (p > 0.05), nor was there a correlation (p > 0.05) between DFI 3 h and PMI 3 h; but %DFI 3 h and HMMP 3 h were significantly correlated (r = ?0.31; p < 0.05). SD‐DFI and %DFI 3 h were the only parameters related to NRR 56 (r = ?0.58; p < 0.05). In conclusion, plasma membranes and mitochondria are similarly affected by the freezing and thawing process, but not during the incubation period after thawing. 相似文献
50.
The health sector's share of the gross national product 总被引:4,自引:0,他引:4
V R Fuchs 《Science (New York, N.Y.)》1990,247(4942):534-538
Between 1947 and 1987 expenditures for health care in the United States grew 2.5 percent per annum faster than expenditures for other goods and services. The health sector's share of the gross national product rose from well under 5 percent in the late 1940s to more than 11 percent in the late 1980s. The expenditures gap has two components: health care prices rose 1.6 percent per annum more rapidly than other prices, while the quantity of health care grew 0.9 percent per annum faster than other quantities. Many factors, including wages, productivity, technology, and insurance contributed to these trends. No single explanation suffices, and no simple solution is apparent. 相似文献