建昌鸭线粒体基因组全序列分析

参照近源物种线粒体基因组序列设计引物15对,用PCR产物直接测序法测得建昌鸭(Anas platyrhychos)线粒体基因组全序列,初步分析其特点和各基因的定位.结果显示:建昌鸭线粒体基因组全长16 606 bp,碱基A、T、G、C的含量分别为29.21%、22.19%、15.77%、32.83%,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码调控区(D-loop),基因组结构和已知雁形目鸟类的完全相同.基于线粒体13个蛋白质编码基因序列,采用邻接法构建雁形目8个物种的系统进化树,所得结果与传统的系统分类基本一致.     

建昌鸭线粒体基因组全序列分析

参照近源物种线粒体基因组序列设计引物15对,用PCR产物直接测序法测得建昌鸭(Arms platyrhychos)线粒体基因组全序列,初步分析其特点和各基因的定位.结果显示:建昌鸭线粒体基因组全长16 606 bp,碱基A、T、G、C的含量分别为29.21%、22.19%、15.77%、32.83%,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码调控区(D-loop),基因组结构和已知雁形目鸟类的完全相同.基于线粒体13个蛋白质编码基因序列,采用邻接法构建雁形目8个物种的系统进化树,所得结果与传统的系统分类基本一致.

Abstract：

The complete mitochondrial genome of Jianchang duck(Anas platyrhychos)was determined by direct DNA sequencing based on the PCR products amplified with 15 pairs of primers designed on related species.The entire genome was found to be 16 606 bp in length and contain 37 genes(13 protein coding genes,2 rRNA and 22 tRNA)and a non-coding control region(D-loop).The content of the nucleotides A,T,G and C was 29.19%,22.20%,15.80%and 32.81%,respectively.The characteristics of the mtDNA of Jianchang duck appeared to be almost identical to those of the other birds of the Anseriformes known.A phylogenetie tree of 8 bird species based on the 13 protein coding genes was then constructed by neighbor-joining analysis.The results were largely consistent with the conclusion in traditional morphological and taxonomic studies.