In vitro Evaluation of Feed Aspergillus sulphureus Enzyme Compound Produced by in Solid-state Fermentation

ABSTRACT： Three trials were conducted to analyze a multi-enzyme compound produced by Aspergillus sulphureus in solid-state fermentation （SSF） as a po- tential feed additive. The results of the first trial showed that there were at least 5 non-starch polysac- charide enzymes： xylanase, 13-ghicanase, pectinase, mannase and carboxy methyl cellulase （CMCase） contained in the compound. Xylanase and fl-glucanase showed good activities at pH 2.5-7.0, which were in the range of 649-1046 U/g and 444-648 U/g, respec- tively. Pectinase showed good activity in acidic solu- tion （pH 2.5-3.0）,which ranged from 195 to 917 U/g. Mannase showed high activity of 235-298 U/g at pH 3.5-4.5 and the activity of CMCase was relatively constant at pH 2.5-7.0, which was in the range of 38.2-78.6 U/g. The second trial was aimed to test the stability of the enzymes in gastric liquor （pH 2.6） of finishing pigs and Na2 HPO4-gastric liquor （ pH 5.5 ）.After 6 h incubation at 40℃ in gastric liquor,the re- tained activity of xylanase, 13-glucanase, pectinase, mannase and CMCase was 26.3% ,65.0% ,71.0%, 74.8% and 85.6%, respectively. While after 6 h in- cubation at 40℃ in Na2I-IPO4-gastric liquor, the re- tained activity of xylanase, [3-glucanase, pectinase, mannase and CMCase was 87.9% ,91.1% ,92.3%, 95.0%, and 97.5%, respectively. The third trial was carried out in a jejunum liquor （ pH 5.8,200 mL）, which contained 0.2 g of the multi-enzyme compound and 10 g of soybean hull or wheat bran, respectively. After 8 h incubation at 40℃, 18.7% of soybean hull and 20.1% of wheat bran could be degraded to solu- ble saccharide, respectively. Compared with the tradi- tional methods for feed enzyme testing which involve feeding animals for 1-3 months, enzyme assay in this way was relatively convenient.     

地表干扰恢复措施对宁南黄土丘陵区典型草原土壤水分入渗特性的影响

[目的]探讨黄土丘陵区地表干扰恢复措施对土壤水分入渗特性的影响。[方法]用双环法测定了宁南黄土丘陵区典型草原鱼鳞坑、水平沟和封育3种恢复措施实施5年后土壤水分入渗特性。[结果]鱼鳞坑、水平沟措施使土壤水分入渗速率显著增加,这与地表干扰导致土壤容重和孔隙度增加有关。水分入渗过程适合用Philip入渗公式模拟。[结论]地表干扰恢复措施可提高土壤水分入渗性能。     

心肌缺血后处理对急性心肌梗死患者血清CK和CK-MB的影响

目的探讨缺血后处理对急性心肌梗死（AMI）患者血清CK和CK-MB峰值及其动态变化的影响.方法将64例12 h内行直接经皮冠状动脉介入治疗（PCI）的ST段抬高型AMI患者随机分为两组,对照组34例,缺血后处理组30例.对照组给予单纯再灌注治疗,缺血后处理组采用再灌注30 s/再缺血30 s,交替3次后再持续灌注的方法.观察两组发病72 h CK和CK-MB峰值及72 h CK值动态变化.结果对照组和实验组CK峰值分别为（1 732.30±480.10）U/L和（1 162.10±547.82）U/L,CK-MB峰值分别为（280.12±99.24）U/L和（164.70±69.67）U/L（P〈0.01）;缺血后处理组72 h CK释放曲线显著低于对照组.结论急性心肌缺血后处理能够显著减少AMI患者CK和CK-MB的释放,从而减轻心肌再灌注损伤.     

美国大豆工业海洋网箱水产养殖技术近海海水鱼类养殖网箱工程技术手册（四十）

4．测试现场测试现场选在Furber海峡,在GreatBayEstuary,NewHampshire（图4）的AdamsPoint海面上。在此河口的这部分区域的水深和水流速度都使它成为一个理想的测试地点。网箱放置在此运河的东侧,离游船的泊位很远。大致的锚位如图所示在下面的航线略图上。在此位置上水流速度应达到2节。     

PIT标记技术在幼鲵标记中的应用研究

对347尾预放流幼鲵的尾部皮下层和肌肉层分别进行横向和纵向PIT标记,通过对标记幼鲵6个月的跟踪观察,共收集到脱落芯片94枚,总脱落率高达27.1%,严重影响标记目的。对植入芯片时的记录进行对比分析,得出尾部皮下层纵向植入法对幼鲵肌体伤害最小,脱落率最低,为3.0%,笔者认为该方法是PIT标记幼鲵的最佳方法。     

草地学计算机辅助教学课件

计算机多媒体技术在教学中的推广应用,极大地提高了教学效果．主要介绍了《草地学》课件的内容、特点和制作过程。     

MicroRNA用于创制抗PRSV番木瓜新种质的展望

微小RNA(microRNA,miRNA)是真核生物体内调控基因表达的一类非常重要的小分子RNA,在植物逆境适应过程中发挥着重要的作用。总结番木瓜环斑病毒(papaya ring spot virus,PRSV)致病机理研究的最新进展,探讨miRNA在植物抗病毒中的研究进展和应用前景,提出研究番木瓜miRNA参与抗PRSV机制的途径,为开展番木瓜miRNA研究和创制抗PRSV番木瓜新种质提供理论参考。     

饲料酶制剂的市场现状和未来

饲料酶制剂通过作用于饲料原料中的抗营养因子,使动物更充分利用饲料中的营养物质,从而提高饲料转化率和肉、蛋的生产效率。此外,饲料酶制剂可减少动物排泄量,在控制畜牧生产中环境污染方面发挥了关键作用。     

薄板木质材料剪切模量的动态检测

为实现薄板木质材料剪切模量的快捷检测,提出了一种悬臂梁自由振动的方法——悬振法,即通过测量试件在悬臂自由振动时的第一阶固有频率和衰减振动波形的对数减幅系数,来检测材料的剪切模量。利用自行研制的薄板类木质材料力学性能快速测量仪,对4种材质、6种规格的薄板木质材料试件进行了检测试验,且将测得的剪切模量与试件的弯曲弹性模量进行了相关性分析。结果表明:悬振法测得的剪切模量与材料弯曲弹性模量间存在非常显著的线性相关关系。      

发酵葡萄渣颗粒饲料对育肥牛生长性能的影响

试验研究了发酵葡萄渣颗粒饲料对育肥牛生产性能的影响。选择26头平均体重为304 kg左右的西门塔尔杂种牛,按体重和性别随机分成2个处理组,每组13头,对照组日粮组成为自配肉牛精料补充料+青贮饲料+干草,试验组日粮组成为发酵葡萄渣颗粒饲料+青贮饲料+干草,试验期为60 d。试验结果表明,与对照组相比,育肥牛饲喂发酵葡萄渣颗粒饲料,日增重提高了5.89%(P0.05),料重比降低了2.79%(P0.05),头均毛利润提高了58.24元,经济效益提高了4.17%。