不同能氮水平的日粮对生长期海南黑山羊采食量和营养物质消化代谢的影响

选用12只海南黑山羊断奶羔羊（约4月龄）分成4组,采用4×4拉丁方设计。按照Keral适宜于发展中国家的山羊饲养标准为参考,设计了高能高氮、低能高氮、高能低氮、低能低氮4种能氮比的日粮,探讨不同能氮水平的日粮对生长期海南黑山羊采食量和营养物质消化代谢的影响。结果表明,不同能氮水平日粮对生长期海南黑山羊营养物质的采食量和消化率都有显著的影响,有的甚至达到极显著水平。     

马铃薯脱毒微型薯组培快繁技术研究

研究首先从5种培养基中筛选出最合适的培养基,即N2培养基,具有制备方便、成本低的优点,适宜于规模化应用;进一步研究了N2培养基对试管苗继代培养以及对不同品种试管苗生长的影响;研究了马铃薯微型原种组培快繁的关键因素,结果表明：马铃薯脱毒微型薯组培快繁的最佳培养基是N2：N1+吲哚乙酸（IAA,5 mg/1000ml）和奈乙酸（NAA,3 mg/1000ml）。     

秦岭山区黄果悬钩子种质资源分析与评价

根据文献记载、野外调查、引种试验和室内分析的结果,对秦岭山区黄果悬钩子种质资源的分布特点、生境条件、形态特征、生态习性、萌芽习性、产量构成、物候期和繁殖特性等方面进行了分析和评价.阐明了黄果悬钩子是一种非常重要的树莓种质资源,具有果大、风味好、营养丰富等优良农艺性状和耐旱、抗寒、矮化、长势强的适应性状.既可以直接开展优良单株的选育与引种驯化栽培,又可以作为一种优良的育种原始材料用作杂交育种的亲本材料.     

关于河套地区小麦近期育种目标的思考

通过对可套地区生态条件、春小麦遗传规律、推广品种更新和性状演变,以及近期育种研究得失的综合分析提出了该地区近期育种目标：“高产、优质、多抗、适应性强”。并解释了品质、抗逆性、适应性等在新品种选育的正确地位。     

玉米粗缩病抗性遗传研究

 本文选用3个抗病自交系(齐319、X178、沈137)和3个感病自交系(掖107、掖478、沈5003)按照NCII交配设计配制9套杂交组合研究了玉米抗粗缩病遗传规律。2009-2010年在曲阳、保定采用田间自然发病方法鉴定亲本、F₁、F₂群体的玉米粗缩病抗性,并采用灰飞虱人工接种方法鉴定亲本材料的抗病性。运用QGA station 软件的加性-显性-上位性(ADAA)遗传模型进行数据分析,结果表明,显性效应和加性效应是控制玉米粗缩病抗性的主要遗传组分,分别占表型变异的44.8%和13.1%,杂合显性效应表现负向杂种优势,抗病育种可加以利用。加性×加性上位性效应在玉米自交系和杂交组合抗粗缩病遗传中普遍存在,但因材料不同而表现负向或正向效应。玉米粗缩病抗性易受环境影响,显性与环境互作效应方差占表型方差的比率为39.8%,达到极显著水平。因此,培育抗粗缩病玉米品种应依据基因型选配适当的亲本材料,抗病品种宜进行多年多点鉴定筛选。     

玉米抗粗缩病遗传及分子标记研究

Maize rough dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is transmitted by planthopper in China. Identification and development of resistant hybrids are complicated because of the inconsistencies in viral disease pressure every year. Marker-assisted selection can provide means for main-taining virus resistance alleles even in the absence of disease. In this paper a F₂ segregation population was constructed to identity the molecular markers linked to the resistance gene using a cross between a resistant and a susceptible parents (Qi319×Ye107). Fifteen-day-old seedlings of F₂ population were exposed to small brown planthoppers carrying RBSDV for 3 days in specific inoculation chamber. The inoculated plants were transplanted to screenhouse after removing the insects completely. In plant maturity stage the disease resistance of all the individuals were visually assessed. The results showed that 17, 8, 11, 51 and 122 plants were scaled from 0-4 respectively, in which 0 means no symptoms and 4 represents highly susceptible. Chi-square test demonstrated that the segregation ratio of phenotype was 1∶15 (resistant: susceptible) or 1∶6∶9 (resistant∶moderate∶susceptible) in the F₂ population, indicating RBSDV resistance of maize was controlled by two recessive genes. The F₂ individuals DNA were extracted and 261 SSR (simple sequence repeat) primers derived from maize genome ten chromosomes were selected from maize GDB database to construct genetic linkage map. The linkage map consisted of 71 polymorphic SSR markers, spanning a genetic distance of 996.6 cM with an average interval of 14.0 cM between adjacent markers. The resistant and susceptible gene pools were set up for BSA (bulked segregant analysis) and 6 polymorphism markers were obtained with BSA-SSR method between the two pools. The F₂individuals were further analyzed with 6 polymorphism markers. Chi-square test showed that phi 051, umc1407 and umc1432, mapped on chromosome 7 and 10, exhibited segregation distortion significantly and very significantly in susceptible individuals. These three SSR markers were identified as potential markers linked to the resistant loci.     

中国花蝽科昆虫多样性及分布格局

在对中国花蝽科昆虫多样性分布统计的基础上,构建数据库,采用特有性简约分析方法,对中国花蝽科昆虫的地理分布格局和物种多样性进行探讨。结果表明,风蝶在中国8个生物地理大区均有分布,且表现出南部比北部丰富、北部比南部原始的特征,华南区和西南区的凤蝶多样性最为丰富。     

全膜微垄垄宽对胡麻经济性状及产量的影响

在半干旱雨养农业区进行了旱地胡麻全膜微垄栽培条件下最佳种植垄宽研究。结果表明,带幅110 cm,每带起2个底宽20 cm、高10 cm和1个底宽40 cm、高10 cm的集雨垄面,在3个集雨垄面之间形成3个底宽10 cm的种植带,用宽120 cm的地膜覆盖,种植带两侧穴播胡麻时胡麻主要性状表现最好,折合产量显著高于其他处理,可达2 863.6 kg/hm~2,较露地穴播增产1 886.3 kg/hm~2,增产率193.01%。     

棚窖的改建及贮藏效果

本文对棚窖的结构进行了分析,找出了影响经济林产品贮藏的原因,并在此基础上对普通棚窖进行了改建,可使贮期延长1个月左右,且贮藏效果较好。     

春小麦数量性状遗传参数的研究

近年国内外对小麦的数量性状遗传规律的研究日渐增多.本文以我区有代性表的水地春小麦品种为材料,研究了各主要数量性状的平均值、遗传型相关系数、遗传进度及相对效率.通过研究分析,初步揭示了春小麦主要数量性状遗传动态和我区小麦育种的经验,为今后新品种选育提供了依据.