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排序方式: 共有168条查询结果,搜索用时 0 毫秒
1.
山羊疥癣病是一种高度接触性体外寄生虫病,发病率高,死亡率低,给发展黄羊产业带来严重的经济损失。我地原用于治疗该病的药物就是敌百虫片混酒精或杀螨灵药浴,品种单一,用药劳动强度大,效果不佳,特别是冬季药浴极不方便,严重制约了山羊业的发展。1 发病情况 凤凰县畜牧水产局、县计委分别于1992年、1994年从四川省南江县两次引种200余只黄羊原种,分别投放到我县的水打田、茶田两乡镇的8村30余户放养,1992~1996年共发疥癣病2508只,死亡38只发病时间多在3~4月、10~11月份,高峰期为3~4月份,10~11月份次之,并以初生3~12月龄… 相似文献
2.
Wang Y Bai Y Qu Q Xu J Chen Y Zhong Z Qiu Y Wang T Du X Wang Z Yu S Fu S Yuan J Zhen Q Yu Y Chen Z Huang L 《Veterinary microbiology》2011,151(3-4):354-362
Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development. 相似文献
3.
Zhanwei Zhuang Jie Wu Yibin Qiu Donglin Ruan Rongrong Ding Cineng Xu Shenping Zhou Yuling Zhang Yiyi Liu Fucai Ma Jifei Yang Ying Sun Enqin Zheng Ming Yang Gengyuan Cai Jie Yang Zhenfang Wu 《畜牧与生物技术杂志(英文版)》2023,(5):1880-1894
Background Pork quality can directly affect customer purchase tendency and meat quality traits have become valuable in modern pork production. However, genetic improvement has been slow due to high phenotyping costs. In this study, whole genome sequence(WGS) data was used to evaluate the prediction accuracy of genomic best linear unbiased prediction(GBLUP) for meat quality in large-scale crossbred commercial pigs.Results We produced WGS data(18,695,907 SNPs and 2,106,902 INDELs exceed quality co... 相似文献
4.
Xiaona Liang Xiaomin Bie Yuliang Qiu Ke Wang Zujun Yang Yuqi Jia Zhiying Xu Mei Yu Lipu Du Zhishan Lin Xingguo Ye 《作物学报(英文版)》2023,(2):573-583
Genetic transformation is widely used to improve target traits and to study gene function in wheat.However, transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew(PM) infection. The widely used recipient variety Fielder is very susceptible to PM. Therefore, it would be beneficial to develop PM resistant derivatives with high regeneration ability for use in genetic transformation. In the present stud... 相似文献
5.
Spear RC Seto E Remais J Carlton EJ Davis G Qiu D Zhou X Liang S 《Science (New York, N.Y.)》2006,314(5802):1081-3; author reply 1081-3
6.
【目的】掌握杉木2代和2.5代种子园的遗传背景信息,为杉木种子园的遗传管理和生产经营提供依据,同时为杉木3代种子园的营建提供遗传信息基础。【方法】以洪雅县国有林场国家杉木良种基地杉木2代及2.5代种子园为对象,通过SSR分子标记技术对其遗传多样性及遗传结构进行分析。【结果】(1)杉木2代种子园平均等位基因数(10.1)、有效等位基因(1.71)和Shannon’s信息指数(1.213)数值要高于大多数杉木和其他针叶树种的种子园或育种资源的数值,表观杂合度(0.440)和期望杂合度(0.478)则低于这些种子园或育种资源的指标值,但单个位点的遗传多样性指标与该位点在其他研究中的表现相比并无明显差异;(2)2.5代种子园相比2代种子园虽然等位基因数量存在一定程度的减少,但其他遗传多样性指标没有出现明显的减少;(3)2代种子园各来源间遗传多样性有一定差异,来源间的遗传分化不明显,基因流(Nm)均大于1,基因交流频繁,变异来源主要存在于群体内(占99%)。110个无性系遗传聚类不明显,遗传背景较为一致。【结论】洪雅国有林场杉木2代种子园具有较丰富的遗传多样性,2.5代种子... 相似文献
7.
Zhuang JX Hu YH Yang MH Liu FJ Qiu L Zhou XW Chen QX 《Journal of agricultural and food chemistry》2010,58(24):12993-12998
Cardol triene was first purified from cashew (Anacardium occidentale L.) nut shell liquid and identified by gas chromatography coupled to mass spectroscopy and nuclear magnetic resonance. The effects of this compound on the activity of mushroom tyrosinase were studied. The results of the kinetic study showed that cardol triene was a potent irreversible competitive inhibitor and the inactivation was of the complexing type. Two molecules of cardol triene could bind to one molecule of tyrosinase and lead to the complete loss of its catalytic activity. The microscopic rate constants were determined for the reaction of cardol triene with the enzyme. The anti-tyrosinase kinetic research of this study provides a comprehensive understanding of inhibitory mechanisms of resorcinolic lipids and is beneficial for the future design of novel tyrosinase inhibitors. 相似文献
8.
Yang Youkui Xiao Changfu Wu Gang Qiu Xiande 《保鲜与加工》1993,(3):30-35
An analysis is made for various kinds of possible breakdown modes during hydrofracture,and the corresponding breakdown pressure,from which a method is given to distinguish which kind of failure mode practically occured. The analysis shows that the initial failure mode at the wellbore wall may be the pure tension failure or shear failure according to different stress states and mechanical properties of rock,therefore there are two different stress state configurations resulting in vertical shear fractures. 相似文献
9.
10.
A new measurement method for mass property parameters 总被引:1,自引:0,他引:1
Mass property parameters (mass, center of mass, moment of inertia) are important technology parameters of aircraft. The traditional mass and center of mass measurement uses centroid units, and the moment of inertia measurement torsion units, which can only get results in installing direction. Because of the structural reasons, there is a need to get all high accurate physical parameters in clamping once. To solve this problem, this study merges centroid units and torsion units into one composite unit, installs a table which can tilt and rotation to measure the moment of inertia in different conditions. The moment of inertia and product of inertia relative to three axis are calculated by the method of coordinate transformation. Measurement theory of mass, center of mass and mass moment of inertia are described, the main reason of causing measurement errors is discussed, and the measurement accuracy affected by tilt error is analyzed. 相似文献