水产品中硝基呋喃类代谢物两种检测方法的比较

通过加标回收实验比较了水产品中硝基呋喃类代谢物检测方法GB 31656.13-2021和农业部783号公告-1-2006公告的差异。实验发现：GB 31656.13-2021中衍生时用0.5 mol/L盐酸,虾蟹样品的AHD有干扰,造成AHD回收率偏高;农业部783号公告-1-2006方法衍生时用0.2 mol/L盐酸,检测结果无干扰,四种硝基呋喃类代谢产物回收率均在正常范围,但对于虾蟹类产品需在加入0.2 mol/L盐酸的基础上再加入盐酸调节溶液pH值1～2才能保证好的衍生效果。     

青稞根腐病防治药剂筛选研究

青稞根腐病在藏区发生日趋频繁、严重,不仅导致青稞大量减产,且对其品质影响颇大。为筛出安全高效的藏区青稞根腐病防治药剂,以藏青2000为指示品种,选取11种常用根腐病防治药剂对其种子拌种或浸种处理进行田间防效试验,田间调查各处理物候期、成株根腐病发病率、防效、农艺性状和折合产量,并基于青稞根腐病发病率、千粒重与折合产量进行Topsis综合评价。结果发现,采用31.9%戊唑·吡虫啉悬浮种衣剂拌种处理(4.8 mL加水200 mL拌种1.6 kg,堆闷24 h)后播种,综合评价最优(Topsis统计量最大,为0.872 726),且植株综合农艺性状优良,根腐病发病率仅4.23%,防效达39.10%,青稞折合产量为2 165 kg/hm2,可考虑在藏区青稞生产中田间防治青稞根腐病时推广使用。     

液相色谱 — 串联质谱法测定海参中的甲氰菊酯和扑草净含量

建立了液相色谱-串联三重四极杆质谱法(LC-MS/MS)同步检测海参中的甲氰菊酯和扑草净.样品用乙腈提取,中性氧化铝柱净化,LC-MS/MS分析,基质标准曲线定量.结果表明,甲氰菊酯和扑草净在0～50 ng/mL范围内呈良好线性,相关系数均在0.999以上,方法检出限为1.0μg/kg,定量限为5.0μg/kg.在5....     

党参茎基腐病镰孢菌鉴定及其对杀菌剂的敏感性研究

为明确党参茎基腐病害的病原、致病性和优势病原种类及其对不同杀菌剂的敏感性,采用组织分离法进行病原菌分离,并根据其培养性状、形态学和分子生物学特征进行鉴定,同时采用平皿菌丝生长抑制法测定不同药剂的敏感性.结果 表明,该病害的病原为尖孢镰孢菌(Fusarium oxysporum)、茄病镰孢菌(F.sonali)和木贼镰孢...     

甘肃选育成功玉米新品种

甘肃省农科院作物所(邮编:730070,电话:0931-7612385)选育的玉米新品种"陇甜1号",前不久通过了甘肃省品种审定。     

掌叶木种子萌发过程中过氧化物酶和超氧化物歧化酶同工酶分析

以掌叶木种子为试材,应用聚丙烯酰胺凝胶垂直平板电泳法,对掌叶木去除种皮的种子萌发过程中过氧化物酶(POD)和超氧化物歧化酶(SOD)同工酶动态变化进行了研究。结果表明:在掌叶木种子萌发初期(0~2d),种子吸水打破休眠而合成少数POD、SOD同工酶;萌发中期(3~5d),种子开始萌动,胚根逐渐伸长,SOD和POD 2类同工酶均有新酶带合成,2类酶的种类、含量迅速增加,标志着种子开始萌发;萌发后期(6~9d),胚根继续伸长,子叶逐渐展开,胚芽开始生长,SOD、POD 2类同工酶酶含量有所减弱,但各同工酶的种类不减,仍有新酶合成。不同萌发时期同工酶的变化可作为掌叶木种子萌发各阶段的重要标志。     

青稞根腐病抗性品种筛选

为筛选抗青稞根腐病的优良品种,结合甘南州青稞栽培种植现状,采用烧杯水琼脂法对37个青稞品种进行室内抗性筛选,对20个青稞品种(系)进行田间抗性鉴定试验,采用Topsis综合评价分析各品种千粒重、抗倒性、产量、根腐病发病率等指标.室内抗性筛选试验结果表明,NQK01-03、碌曲紫青稞和黄青1号为抗病品种,黄青2号、昆仑1...     

Identification and biological characterization of the pathogens responsible for sclerotinia rot in Codonopsis pilosula北大核心CSCD

The aim of this study was to isolate and characterize the causal pathogen and study the biological characteristics of sclerotinia rot of Codonopsis pilosula. Pathogen material was obtained by tissue isolation. The isolated fungal species were comprehensively evaluated in terms of their morphology,rDNA-ITS sequence,RPB2 gene of RNA polymerase Ⅱ,and biological characteristics. The pathogens were identified as Sclerotinia nivalis and Sclerotinia sclerotiorum,and of the two,S. nivalis was the dominant species. The optimum temperatures for mycelium growth and sclerotia formation of S. nivalis were 20 and 15 ℃,respectively,while corresponding values for S. sclerotiorum were 25 and 25 ℃ . The optimum pH for mycelium growth was 6 for both species,while optimum pH for sclerotia formation was 6 for S. nivalis and 7 for S. sclerotiorum. The optimum culture medium was 100% potato dextrose agar（PDA）. The mycelial growth rate approach was applied to test the toxicity of adiacylamine, which exhibited inhibitory activity with EC50 on S. nivalis at 0. 2625 mg·L-1 and on and S. sclerotiorum at 0. 4165 mg·L-1. In summary,this is the first report of S. nivalis and S. sclerotiorum causing sclerotinia rot on C. pilosula in China,and the data presented provide a reliable scientific proof of the pathogen diagnosis and a theoretical basis for the development of prevention measures. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

4LZ-5G型谷子联合收获机在古县丘陵山区应用的技术性能考核

介绍了4LZ-5G型谷子联合收获机试验考核情况,得出了试验考核结论,分析了存在的问题并提出了改进建议,对经济效益进行了评估。     

植物与家居搭配技巧

现在,越来越多的人们喜欢在家里放上几盆植物。除了看上去赏心悦目之外,在家里摆放植物还能净化室内空气。但众所周知的是,绿色植物在白天放出氧气、吸入二氧化碳等气体;到了晚上则是吸入氧气,排