黄芪多糖对猪瘟疫苗免疫效果影响的Meta分析

为了系统评价黄芪多糖对猪瘟疫苗免疫效果的影响,通过检索数据库资料,纳入有关黄芪多糖对猪瘟疫苗免疫效果影响的随机对照试验(共纳入11个对照试验,样本量为244头猪),利用Cochrane Reviewer′s Handbook 5.0评价标准对试验质量进行评价,并使用Revman 5.3进行统计分析。结果显示:黄芪多糖可显著提高血液中的猪瘟抗体水平,同时提高其血液中的IL-2含量,优于单纯免疫组。表明黄芪多糖可通过提高机体的体液免疫和细胞因子水平来增强猪瘟疫苗的免疫效果,效果确切,值得推广应用。     

猪发育期NPY-Y1和GPR54受体基因的表达规律研究

[目的]研究猪发育期NPY-Y1和GPR54受体基因的发育性变化。[方法]分别选取初生、60、120、初情期、180日龄的苏姜猪母猪各3头,采集下丘脑、垂体、卵巢组织样品,提取组织RNA,采用荧光定量PCR分析NPY-Y1、GPR54受体基因的发育性变化。[结果]下丘脑、垂体与卵巢内NPY-Y1 mRNA从初生到初情期表达量逐渐下降,在初情期达到最低,初情期后呈上升趋势。在3种组织内,初情期与其他时期NPY-Y1 mRNA表达量均存在显著差异(P<0.05);GPR54 mRNA从初生到初情期表达量逐渐上升,在初情期达到最高,初情期后呈下降趋势。在3种组织内,初情期也与其他时期GPR54 mRNA表达量也均存在显著差异(P<0.05)。[结论]猪发育期NPY-Y1基因与GPR54基因表达存在负相关性。     

阿奇霉素联合黄芪多糖对姜曲海仔猪气喘病的疗效

应用阿奇霉素联合黄芪多糖对患有气喘病的姜曲海仔猪进行治疗试验,为姜曲海仔猪气喘病防治提供新的治疗药物。结果表明:阿奇霉素联合黄芪多糖治疗组的治愈率高于泰乐菌素治疗对照组和阿奇霉素治疗对照组(P<0.05);平均日增重显著高于感染对照组(P<0.01)、泰乐菌素治疗对照组(P<0.01)和阿奇霉素治疗对照组(P<0.05)。表明,阿奇霉素联合黄芪多糖对姜曲海仔猪的气喘病治疗效果显著,可作为治疗姜曲海猪气喘病的主要用药。     

MCC950 Improves Growth Performance, Ｕric Acid Metabolism and Kidney Injury of Goslings Fed High Protein Diet by Inhibiting Nucleotide Binding Oligomerization Domain-Like Receptor 3 Inflammasome北大核心CSCD

The aim of this study was to evaluate the effects of MCC950 on growth performance, uric acid metabolism, serum inflammatory factor contents and kidney injury of goslings a fed high protein diet, and explore the potential mechanism of nucleotide binding oligomerization domain-like receptor 3 (NLRP3) / cysteine aspartase-1 (Caspase-1) signaling pathway in gosling gout. A total of 120 Yangzhou goslings of 1-day-old were randomly divided into 3 groups, which were control group, high protein group (HP group) and high protein+ MCC950 group (HP+MCC950 group), with 5 replicates in each group and 8 goslings in each replicate. The control group was fed a normal protein diet with 16.5% crude protein, and the HP group and HP+MCC950 group were fed a high protein diet with 24.0% crude protein. The HP+MCC950 group was intraperitoneally injected with MCC950 every 3 days at a dose of 10 mg / kg BW, and the other two groups were injected with the same amount of normal saline. The experiment lasted for 15 days. The results showed as follows: 1) compared with the control group, the average body weight of goslings at 15 days of age in the HP group was significantly decreased (P<0.05); the serum contents of uric acid (UA), creatinine (Cr), urea nitrogen (UN), C-reactive protein (CRP), interleukin-1β (IL-1β), interleukin 18 (IL-18), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1) in the HP group were significantly increased (P<0.05); the mRNA relative expression levels of NLRP3, Caspase-1, IL-1β and IL-18 and the protein relative expression levels of NLRP3, Caspase-1 and IL-1β in kidney tissues in the HP group were significantly increased (P<0.05). 2) Compared with the HP group, the average body weight of goslings at 10 and 15 days of age in the HP+MCC950 group was significantly increased (P<0.05); the serum contents of UA, Cr, UN, CRP, IL-1β, IL-18, IL-6, TNF-α and TGF-β1 were significantly decreased (P<0.05), while the mRNA relative expression levels of NLRP3, Caspase-1, IL-1β and IL-18 and the protein relative expression levels of NLRP3, Caspase-1 and IL-1β in kidney tissues in the HP+MCC950 group were significantly decreased (P< 0.05). 3) Histological findings showed that the renal tubules of goslings were dilated, and vacuolar degeneration and necrosis occurred in the HP group, while the structure of renal tubules was relatively normal in the HP+MCC950 group. The urate deposition area in kidney tissues of goslings in the HP group was significantly larger than that in the control group (P<0.05), and which in the HP+MCC950 group was significantly smaller than that in the HP group (P<0.05). Moreover, the urate deposition area in kidney tissues of goslings was significantly positively correlated with the protein relative expression level of NLRP3 (r = 0.681, P<0.05). In conclusion, MCC950 can promote the growth of goslings fed a high protein diet and reduce the serum uric acid content, which may be related to the reduction of inflammatory response and kidney injury through its inhibition of NLRP3 / Caspase-1 signaling pathway. © 2023 Authors. All rights reserved.     

职业教育国家教学资源库数字化资源的开发与应用

以现代宠物技术国家资源库建设为个案,阐述了资源库数字化资源开发的一体化顶层设计、结构化课程体系建设及颗粒化资源建设等方面的建设思路与方法,总结经验,为高职教育的教学改革和教学实施提供范例.     

培养兽医临床思维提高化验诊断技术教学质量

传统的兽医化验技术只注重操作过程考核,对检测结果分析能力考评不足,影响动物医学(宠物医学)专业学生教学质量.本文从临床意义、实验数据及与其他学科关联性等方面进行分析,探讨如何培养学生临床思维能力.     

大豆异黄酮对去势雌性犬血脂代谢及抗氧化能力的影响

研究证明,雌性动物去势后可导致脂肪代谢障碍[1],大量脂肪在体内堆积,引起肥胖,这是由雌激素分泌减少,血液雌激素水平下降,下丘脑-垂体-性腺轴平衡失调造成的。近几年,宠物临床实践亦证明,绝育雌性犬发生肥胖症的概率是正常犬的2倍,     

农业高职院毕业生顶岗实习现状调查研究

顶岗实习是高职院校培养人才至关重要的环节,通过顶岗实习,校企密切合作,才能有效推进工学结合人才培养模式取得成效,确保人才培养的质量.本调查以我校2017届动物医学专业为研究对象,从农业高职院校学生顶岗实习相关的各个角度和方面进行全面调查统计,详细分析农业高职院毕业生顶岗实习中存在的各种问题,为加强顶岗实习管理和提高岗顶实习效果提供依据.     

绝育手术对雌性Beagle犬血脂代谢及抗氧化能力的影响

观察绝育手术与母犬血脂代谢及抗氧化能力之间的关系,为防治母犬绝育后肥胖症提供理论支持。将15只成年雌性Beagle犬随机平均分为对照组、假手术组、绝育组,定期测定绝育后血清脂肪代谢指标及抗氧化指标。结果表明:(1)绝育组血清甘油三酯、总胆固醇、低密度脂蛋白的含量逐渐增高,与时间呈正相关,且术后30 d显著高于对照组及假手术组(P<0.05),术后60 d极显著高于对照组及假手术组(P<0.01);而高密度脂蛋白的含量逐渐降低,与时间呈负相关,且术后30 d显著低于对照组及假手术组(P<0.05),术后60 d极显著低于对照组及假手术组(P<0.01)。(2)绝育后60 d,血清谷胱甘肽过氧化物酶7及超氧化物歧化酶水平显著低于对照组及假手术组(P<0.05),而丙二醛含量显著高于对照组及假手术组(P<0.05)。说明绝育手术可增加母犬的血脂浓度,且减弱机体抗氧化能力。