表达小反刍兽疫病毒H基因的山羊痘病毒通用转移载体的构建及鉴定(英文)

[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.     

变频调速供水技术在乡镇供水中的应用

鹿泉市方台经济开发区水厂设有 4台双吸离心泵 10ＳＨ -6Ａ型 ,配套电机 110ｋＷ ,运行方式为三用一备形式 (检修备用 )。电气设备配置两台机组配备变频调速装置 ,两台机组配备自耦变压器降压启动装置。即 1号、2号机组配备变频调速装置 ,作变速运行 ,3号、4号机组配备降压启动装置 ,作定速运行。变频调速装置输出 4对接点 ,经过 4个连接片 ,分别接到两台定速运行电机的开机和停机回路。变频调速装置设有ＰＬＣ可编程控制器 ,系统开始运行时 ,变频调速装置首先启动 ,水压传感器把实测水压值输入变频调速装置与设定的压力值进行比较、运算 ,…     

基于地形与遥感辅助信息的小流域尺度高分辨率有机碳空间分布预测研究

土壤有机碳(soil organic carbon,SOC)既是衡量土壤质量的重要指标，也是影响全球碳氮循环的关键因素之一。作为数字土壤制图(digital soil mapping,DSM)研究中起主要作用的环境变量，地形元素在SOC预测制图中也是无可替代的。应用机器学习模型，通过引入不同超参数设置下获得的高分辨率(5 m)Geomorphons(GM)地形分类图作为丘陵地形特征信息的补充，结合数字高程模型(digital elevation model,DEM)衍生变量和光学、合成孔径雷达(synthetic aperture radar,SAR)遥感数据对句容市黄梅镇北部小流域尺度(1︰25 000)丘陵地貌区地表层SOC含量进行预测制图，并评估不同GM变量在SOC含量预测中的表现。基于74个土壤样本和不同环境变量组合，分别采用袋装决策回归树(bagged classification and regression tree,bagged CART)、随机森林(random forest,RF)和立体派(cubist)三种方法构建SOC含量预测模型，并通过四个精度验证指标，采用十...