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61.
Sari?JuutinenEmail author Tuula?Larmola Rainer?Remus Edith?Mirus Wolfgang?Merbach Jouko?Silvola Jürgen?Augustin 《Biology and Fertility of Soils》2003,38(1):10-14
The contribution of Phragmites australis (Cav.) Trin. ex Steud. (common reed) litter as an origin of CH 4-C was studied in a microcosm experiment. 14C-labelled, dried and ground P. australis root and shoot litter was buried in waterlogged, planted or non-planted fen microcosms. The evolution of 14CO 2 and 14CH 4 from the pots was monitored during the 35-day experiment. The 14C activity in the shoots and roots of the plants, soil, and inundation water was also analysed at the end of the experiment. Up to 40% of the released CH 4-C originated from the added litter, whereas the rest originated from old soil organic matter. The comparison of planted and non-planted pots suggested that the contribution of recent plant-derived C (i.e. root exudates) to CH 4 emission was negligible. The proportion of litter-derived CH 4-C was significantly higher in the planted pots, suggesting that the presence of plants enhanced the formation of CH 4 from litter. The major part of the initial 14C activity was recovered from the soil. About 3% was recovered from the inundation water, about 10% was emitted as CO 2, and only <0.01% as CH 4. However, these results demonstrated that plant litter and old soil organic matter are the major sources of CH 4-C in fens during the early growth stage of P. australis. 相似文献
62.
Effects of genetically modified plants on microbial communities and processes in soil 总被引:16,自引:0,他引:16
M.?Bruinsma G.?A.?Kowalchuk J.?A.?van?VeenEmail author 《Biology and Fertility of Soils》2003,37(6):329-337
The development and use of genetically modified plants (GMPs) has been a topic of considerable public debate in recent years. GMPs hold great promise for improving agricultural output, but the potential for unwanted effects of GMP use is still not fully understood. The majority of studies addressing potential risks of GMP cultivation have addressed only aboveground effects. However, recent methodological advances in soil microbial ecology have allowed research focus to move underground to try to gain knowledge of GMP-driven effects on the microbial communities and processes in soil that are essential to key terrestrial ecosystem functions. This review gives an overview of the research performed to date on this timely topic, highlighting a number of case studies. Although such research has advanced our understanding of this topic, a number of knowledge gaps still prevent full interpretation of results, as highlighted by the failure of most studies to assign a definitively negative, positive or neutral effect to GMP introduction. Based upon our accumulating, yet incomplete, understanding of soil microbes and processes, we propose a synthesis for the case-by-case study of GMP effects, incorporating assessment of the potential plant/ecosystem interactions, accessible and relevant indicators, and tests for unforeseen effects. 相似文献
63.
Annemie?ElsenEmail author Raf?Beeterens Rony?Swennen Dirk?De?Waele 《Biology and Fertility of Soils》2003,38(6):367-376
In this study, the effect of an arbuscular mycorrhizal fungus (AMF) and two migratory endoparasitic nematodes on Musa plant growth, including the root system, were examined. In addition, the AMF-nematode interaction was studied. Seven Musa genotypes with different root systems were selected. Based on their relative mycorrhizal dependency, two genotypes (Calcutta 4 and Obino l'Ewai) were selected for AMF-nematode interaction studies. The experiments were performed under greenhouse conditions. Mycorrhization with Glomus mosseae resulted in a significantly better plant growth even in the presence of nematodes. The effect of AMF on the root system was genotype-dependent and seemed to be related to the relative mycorrhizal dependency of the genotype. The nematodes also affected the root system, decreasing branching. Nematode population densities were significantly reduced in the presence of AMF, except for Pratylenchus coffeae in Obino l'Ewai. In the root system, it appeared that the decreased branching caused by the nematodes was counterbalanced by the increased branching caused by the AMF. 相似文献
64.
Jason?M.?ConderEmail author Roman?P.?LannoEmail author 《Journal of Soils and Sediments》2003,3(1):13-20
Background. Earthworm heavy metal concentrations (critical body residues, CBRs) may be the most relevant measures of heavy
metal bioavailability in soils and may be linkable to toxic effects in order to better assess soil ecotoxicity. However, as
earthworms possess physiological mechanisms to secrete and/or sequester absorbed metals as toxicologically inactive forms,
total earthworm metal concentrations may not relate well with toxicity.
Objective The objectives of this research were to: i) develop LD50s (total earthworm metal concentration associated with 50% mortality) for Cd, Pb, and Zn; ii) evaluate the LD50 for Zn in a lethal Zn-smelter soil; iii) evaluate the lethal mixture toxicity of Cd, Pb, and Zn using earthworm metal concentrations
and the toxic unit (TU) approach; and iv) evaluate total and fractionated earthworm concentrations as indicators of sublethal
exposure.
Methods Earthworms (Eisenia fetida (Savigny)) were exposed to artificial soils spiked with Cd, Pb, Zn, and a Cd-Pb-Zn equitoxic mixture to estimate lethal CBRs
and mixture toxicity. To evaluate the CBR developed for Zn, earthworms were also exposed to Zn-contaminated field soils receiving
three different remediation treatments. Earthworm metal concentrations were measured using a procedure devised to isolate
toxicologically active metal burdens via separation into cytosolic and pellet fractions.
Results and Discussion Lethal CBRs inducing 50% mortality (LD50, 95% CI) were calculated to be 5.72 (3.54-7.31), 3.33 (2.97-3.69), and 8.19 (4.78-11.6) mmol/kg for Cd, Pb, and Zn, respectively.
Zn concentrations of dead earthworms exposed to a lethal remediated Zn-smelter soil were 3-fold above the LD50 for Zn and comparable to earthworm concentrations in lethal Zn-spiked artificial soils, despite a 14-fold difference in total
soil Zn concentration between lethal field and artificial soils. An evaluation of the acute mixture toxicity of Cd, Pb, and
Zn in artificial soils using the Toxic Unit (TU) approach revealed an LD50 (95% CI) of 0.99 (0.57-1.41) TU, indicating additive toxicity.
Conclusions Total Cd, Pb, and Zn concentrations in earthworms were good indicators of lethal metal exposure, and enabled the calculation
at LD50s for lethality. The Zn-LD50 developed in artificial soil was applicable to earthworms exposed to remediated Zn-smelter soil, despite a 14-fold difference
in total soil Zn concentrations. Mixture toxicity evaluated using LD50s from each single metal test indicated additive mixture toxicity among Cd, Pb, and Zn. Fractionation of earth worm tissues
into cytosolic and pellet digests yielded mixed results for detecting differences in exposure at the sublethal level
Recommendation and Outlook CBRs are useful in describing acute Cd, Pb, and Zn toxicity in earthworms, but linking sublethal exposure to total and/or
fractionated residues may be more difficult. More research on detoxification, regulation, and tissue and subcellular partitioning
of heavy metals in earthworms and other invertebrates is needed to establish the link between body residue and sublethal exposure
and toxicity. Keywords: Bioavailability; Cd; critical body residues; earthworms; metals; Pb; soil; Zn
An erratum to this article is available at . 相似文献
65.
Background, Aims and Scope Disposal of dredged material in subaqueous depots is increasingly considered an economic and ecologically sound option in
managing contaminated dredged material. The concept of subaqueous disposals capped with active barrier systems has been developed
to minimize this risk of contaminant release. As such a depot represents a permanent installation within a sensitive ecosystem,
it requires a thorough monitoring concept. It is the goal of this work to develop such a concept regarding general considerations
and results of laboratory and field investigations.
Methods In addition to the state-of-the-art techniques developed for other under-water constructions, this monitoring concept is developed
with particular respect to the chemical isolation of the dredged material from the overlying water body. It comprises the
use of seepage meters, dialysis samplers, and DGT gel probes for determining the migration of selected target solutes. The
capability of the dialysis samplers is demonstrated by comparing field results with model calculations. The appropriateness
of DGT probes to assess the impact of humic substances on trace metal speciation and on copper toxicity is demonstrated with
the aid of laboratory experiments.
Results and Discussion The experimental results show that, by using dialysis samplers, the temporal changes in concentration-depth-profiles of heavy
metals in the pore solution can be monitored. Additionally, the application of DGT probes facilitates the in situ detection
of labile species of a metal in the presence of dissolved humic substances, which serves to reflect its toxicity. Conclusions.
Three subsequent monitoring phases are distinguished on the basis of both general considerations and the findings from field
results: A hydraulic phase that is characterized by compaction and pore water expulsion, a geochemical phase in which the
demobilization of pollutants can occur due to substantial changes in the physico-chemical conditions (pH, EH), and a steady-state-phase
where pore water flow and geochemical conditions are approaching their minimum.
Recommendations and Outlook The monitoring concept suggested here provides a versatile tool to assess the chemical isolation of subaqueous sediment depots
and other contaminated sediment sites. This is of great importance as subaqueous disposal is increasingly considered a future
management strategy as space for upland disposal is limited and treatment, in general, proves to be too costly. 相似文献
66.
Kay?HamerEmail author Astrid?Hadeler Thomas?Muschalla Jürgen?Schr?ter Günter?Timmer 《Journal of Soils and Sediments》2003,3(4):284-291
Goal
Annually, 400.000 m3 harbour sediments are dredged to maintain the water depth in the harbours of Bremen and Bremerhaven. The sediment contains organic and inorganic pollutants and hence is deposited on a landfill. Because of the limited capacity of that landfill alternative treatment techniques are investigated. This study aims to evaluate the production of Light Weight Aggregates (LWA) from harbour sediments with respect to the product quality and environmental aspects of the use of the LWA. 相似文献67.
Sujit?K.?GhoshEmail author 《Journal of Agricultural, Biological & Environmental Statistics》2003,8(3):367-382
This article considers statistical modeling of a simplified version of Darwinian evolution which involves only two genes encoding
one molecule. In particular, we consider a process known as affinity maturation that is carried out in the germinal center reaction (GCR). We use a statistical model, developed earlier, that enables us to tease apart the effects of mutation rate and selection
in B cell populations undergoing an immune response. Standard classical statistical approaches that use large-sample theory
may lead to misleading conclusions when sample size is very small. The data collection in many biological systems, including
the immune response of vertebrate organisms, is both time consuming and often results in relatively small datasets. Therefore,
this article takes a Bayesian perspective that produces finite-sample estimates of the mutation rates and is flexible in extending
the models to more complicated processes. The method is illustrated using sequence data obtained from Gen bank that carried
the experiment over two years. 相似文献
68.
Effects of N sources and methane concentrations on methane uptake potential of a typical coniferous forest and its adjacent orchard soil 总被引:6,自引:0,他引:6
After removal of the above-ground plant debris three different soil layers were taken from a typical coniferous forest and its adjacent orchard in Numata City, Japan. The potentials of soil CH4 uptake at two initial CH4 concentrations were studied under aerobic conditions in the laboratory, along with inhibition of soil CH4 oxidation by urea-N or KNO3-N addition. Due to long-term N inputs, the CH4 uptake of the upper mineral layer of the orchard soil was 25.4% and 87.7% lower than that of the surface forest soil at 2.4 and 12.6 l l–1 CH4, respectively. Methane uptake of the forest soil decreased with increasing soil depths at two CH4 levels. However, maximal CH4-consuming activity occurred in the 9- to 23-cm depth of the orchard soil at 12.6 l l–1 methane. Nitrogen additions in the form of KNO3 or urea at the rate of 200 g N g–1 soil substantially reduced soil CH4 uptake in the upper and sub-surface mineral layers at both sites, except that the addition of KNO3-N had no apparent inhibitory effect on the CH4 uptake in the 9- to 23-cm depth of the orchard soil. A strong inhibitory effect of NO3– addition on the CH4 uptake, in contrast to NH4+, occurred in the surface forest soil. The use of KNO3-N, as compared to urea or urea plus a nitrification inhibitor (dicyandiamide), resulted in a lower potential to cause inhibition of CH4 oxidation in the 0- to 23-cm depth of the orchard soil. 相似文献
69.
Minna-Liisa?RantalainenEmail author Hannu?Fritze Jari?Haimi Oili?Kiikkil? Taina?Pennanen Heikki?Set?l? 《Biology and Fertility of Soils》2004,39(3):200-208
Due to their high abundance and ubiquitous existence, microbes are considered to be efficient colonisers of newly established habitats. To shed light on the dispersal mechanisms of soil microbes, a controlled microcosm experiment was established. In these microcosms, the dispersal of microbes from a source humus patch to originally sterile humus patches (embedded in a mineral soil matrix) was followed for 16 months, applying 16S and 18S ribosomal DNA-based PCR-DGGE molecular methods. Specifically, the role of enchytraeid worms and habitat (humus) corridors as possible facilitators of microbe dispersal was studied. The results showed that enchytraeid worms function efficiently as vectors for horizontal dispersal of saprophytic fungi in soil. Some of the fungi also proved to disperse through the corridors by vegetative growth, although this dispersal was inefficient as compared to dispersal with the enchytraeids. Virtually no saprophytic fungi were able to disperse through the mineral soil matrix in the absence of both enchytraeid worms and corridors. Unlike soil fungi, the dispersal of soil bacteria was not affected by any of the studied factors. The results of the present experiment provide direct evidence of the crucial role of soil fauna in aiding the horizontal dispersal of soil fungi. The role of enchytraeids as a functionally important species in boreal forest soils is further emphasized, since bringing microbes into contact with new resources is likely to enhance the rate of decomposition in soils. 相似文献
70.
A greenhouse experiment was conducted with a specially designed apparatus consisting of an upper and lower chamber where the treatment with rice was carried out (treatment 1). The apparatus also had a single chamber where treatment 2, without rice plants, was carried out. The scope of this study was to elucidate the influence of rice plant growth on gaseous N losses as N2 and N2O produced by nitrification-denitrification in a flooded soil fertilized with (NH4)2SO4 (with 56.50 atom% 15N). Gas samples were withdrawn weekly and analyzed for (N2 + N2O)-15N losses by mass spectrometer and for N2O by gas chromatograph. The gaseous (N2 + N2O)-15N losses of the treatment with rice plants were significantly (P =0.01) higher than those of the treatment without rice plants, as were the amounts of N2O emitted. Rice plants facilitate the efflux of N2 and N2O from soil to atmosphere, as about half of the total gaseous 15N loss as N2 and N2O was found in the upper chamber. The proportion of N2O-15N to (N2 + N2O)-15N in the upper chamber was 10.56%, much higher than that of the lower chamber in treatment 1 and the headspace of treatment 2. 相似文献