CX饲用蛋白粉被确定为国家重点新产品

国家科技部将天津市创新饲料有限公司开发的新型蓖麻粕脱毒生产线及其工艺,以及其生产的ＣＸ饲用蛋白粉列入2000年度国家重点新产品计划,确定为国家重点新产品。该生产线采用机械、热力及化学综合脱毒工艺,将蓖麻榨油后的残渣加工成优质植物蛋白饲料原料———创新牌ＣＸ饲用蛋     

应用分子标记筛选辣椒雄性不育恢复系研究

利用SCAR 引物对359 份未知育性的辣椒进行恢复基因的鉴定,结果显示,有35 份材料有恢复 基因特异条带,占比9.75%。恢复基因主要分布在小果型的辣味型品种和部分长果型（牛角形、羊角形）品种 中。品种果实越大（横径越大）恢复基因越少,出现育性不稳定的比例也越高,即雄性不育系与亲缘关系较近 的品种杂交易恢复,与亲缘关系较远的品种杂交易保持相一致。     

2001年我国小麦市场分析

一、生产情况 2001年我国小麦继续减产的可能性相当大。据农业部对全国600多个县秋冬播种面积的统计调查，1999年粮食作物中冬小麦面积预计为2 366.7 万hm2，比2000年减少180多万hm2。预计2000年冬小麦中优质小麦的播种面积仍将有所增加，但总播种面积将继续减少，有关部门估计，减幅将为5%左右。另据气象部门提供的信息显示，当前北方大部分地区的小麦越冬状况属于近年来较差的年份。由于2000年秋季小麦播种期因持续阴雨天气而明显延迟，加之入冬以后，北方地区气温下降较快，使得冬小麦的冬前分蘖数量普遍不如1999年同期。此外，由于我国在2000年继续降低了小麦的收购价格，使得农民种植小麦的积极性受到影响，物质投入下降，也在一定程度上对小麦越冬状况构成了影响。但入春以来北方冬麦区出现了较大范围的降雪，增加了土壤水分，有利于冬小麦返青生长。 二、库存状况 自1996年以来，我国小麦产量已连续4年保持在1.1亿t以上，虽然进口数量逐年减少，但国内小麦年度结转库存量仍维持在2 500万t以上，年供应量仍维持在1.1亿～1.2亿t左右。     

抗猪瘟高免血清的制备及应用

1　抗猪瘟高免血清的制备　选取体况和健康状况一致的试验猪为供试猪进行首次免疫,将供试猪颈部肌注猪瘟兔化弱毒苗10头份/头猪,观察免疫后猪只的精神、食欲等变化。10天后耳静脉采血,Ｄｏｔ-ＥＬＩＳＡ诊断试剂盒测效价达1∶80。二免猪瘟兔化弱毒苗20头份/头猪,15天后采血测效价达1∶320。三免用猪瘟野毒强毒颈部肌注1∶10脾组织悬液10ｍＬ/头,20天后采血测效价达1∶1280。器械消毒准备完成后,颈总动脉放血致死,分离血清,加入2000ｉｕ/ｍＬ双抗,0.01%硫柳汞,即制成抗猪瘟高免血清,分装于-15℃冷冻保存备用。(见表1)表1　抗猪瘟高免血清的制…     

不同浓度玻璃化冷冻保护液及冷冻载体对驴卵母细胞成熟率的影响

为了比较不同冷冻液及冷冻载体对驴卵母细胞成熟率的影响,试验设计4个浓度梯度（Ⅰ、Ⅱ、Ⅲ、Ⅳ）冷冻液和3种冷冻载体分别对生发泡（germinal vesicle,GV）期驴的卵母细胞进行玻璃化冷冻,对解冻后的卵母细胞进行体外成熟,通过比较卵母细胞的成熟率反映其冷冻效果。结果表明,冷冻液Ⅱ的卵母细胞成熟率（28.13%）显著（P〈0.05）高于其他3组,但显著（P〈0.05）低于正常组（51.77%）,在不同冷冻载体对驴卵母细胞成熟率的影响试验中,使用开放式拉长塑料细管（OPS）的卵母细胞成熟率（28.13%）显著（P〈0.05）高于普通玻璃细管组（11.67%）,但与GMP管组（23.53%）差异不显著（P〉0.05）。     

Transcriptome Profiling and Biochemical Studies Reveal New Mechanisms for Cotton Fiber Cell Elongation

Cotton is the world＇s most important natural textile fiber, and is practiced on about 2.5% of arable land that supported the life of about 100 million family units. Each cotton fiber, about 25 000 per seed, is a single, phenomenally elongate, epidermal cell of the cotton seed. Biologically, cotton fiber is an excellent model system for plant cell elongation and cell wall and cellulose biosynthesis （Qin et al., 2005; 2007; Xu et al., 2007）. The fiber is composed of nearly pure cellulose, which is also the largest component of plan（biomass （Lu et al., 2002）. Here, through sequencing of a cotton fiber eDNA library and subsequent microarray analysis, we found that ethylene biosynthesis is one of the most significantly up regulated biochemical pathway during the fiber elongation period. ACO1-3 genes responsible for ACC （1-aminocyclopropane-l-carboxylic acid） oxidation and ethylene production were expressed at significantly higher levels during this growth stage. The amount of ethylene released from cultured ovules correlated with ACO expression and the rate of fiber growth. Exogenously applied ethylene promoted robust fiber cell expansion,     

Transgene Homozygosity Following Particle Bombardment of Haploid Barley Microspores

Information from previously published studies that are basic to this study is： 1） Following isolated barley microspore culture, around 80% of the resulting barley plants are completely fertile and genetically homozygous doubled haploids （DH） （Kasha et al., 2003）. 2） Chromosome doubling occurred during early stages of microspore culture, mainly by nuclear fusion （Kasha et al., 2001）. 3） A fairly synchronous population of microspores was selected to determine the cell cycle stages （G1, S, G2） by relative DNA density measurements （Shim and Kasha, 2003a）. 4） The pretreatments of spikes used to induce microspore embryogenesis influenced the cell cycle progression during pretreatment. （i） Using a combination of cold （4℃） and 0.3 mol/L mannitol for 4 days, the microspores were held at the G1 uninucleate stage. （ii） The often used pretreatment of cold （4℃ for 21 to 28 d） permitted slow microspore stage progression so that most cells were at the G2 stage or had become binucleate by the end of the pretreatment. （iii） Pretreatment with mannitol for 4 days at 25 ℃ did not block cell cycle progression but prevented cell wall formation after the 1^st mitotic division, leading to nuclear fusion and chromosome doubling.