水稻钙依赖型蛋白激酶OsCPK9基因启动子的克隆及在烟草中的瞬时表达分析

植物钙依赖型蛋白激酶(CDPK)调控钙信号途径下游组分,与植物的生长发育及各种逆境生理过程密切相关。通过对本课题组克隆的水稻OsCPK9基因的cDNA序列与NCBI中的水稻基因组数据库进行比对、定位,结合生物信息学的方法,预测到基因上游的一段启动子序列。进而利用PCR的方法从水稻‘日本晴’(Oryza sativa L. cv. Nipponbare)基因组DNA中克隆到了水稻OsCPK9基因5’端上游约2 kb的DNA序列,命名为POsCPK9。PLANTCARE在线分析表明,POsCPK9序列除包含植物启动子所必备的基本元件如TATA box 和CAAT box外,还含有多个与逆境和信号物质相关的顺式表达元件。将克隆到的POsCPK9取代pBI121中的CaMV 35S 启动子,构建成POsCPK9与GUS的融合表达载体POsCPK9 GUS;通过农杆菌介导的方法在烟草的根、茎、叶中进行瞬时表达。结果显示,该启动子驱动的GUS基因在烟草的根、茎、叶中都有不同程度的表达。说明OsCPK9基因上游2 kb具有启动子活性。     

科学运作互利共赢——山东巨野大力推广机械化玉米秸秆青贮技术

近年来,巨野县出台一系列惠农政策,助推农业、畜牧业迅猛发展,特别是县东南部6个镇区,在陶庙镇夏庄、夏苗庄2个民族村(回族)的带动下,"架子羊"、"架子牛"养殖业已初具规模.     

基于遗传算法的生理信号情感识别

针对用生理信号来识别情感状态中的最优情感特征组合选择这一组合优化问题,用遗传算法来选择最能代表相应情感状态的最优特征组合,以最近邻法的分类正确率作为当前搜索到的最优特征组合评价准则,将两者结合用于joy,anger,pleasure,sadness4种情感状态的识别,得到了较好的情感识别效果．仿真实验表明,该方法是有效的．     

硒对镉胁迫下水稻幼苗部分生理特性的影响

采用溶液培养 ,研究硒和镉对水稻幼苗长度、苗鲜重、叶绿素含量以及叶片N、P、K含量的影响 .结果表明 ,单用镉处理可使水稻幼苗矮化 ,苗鲜重降低 ;高浓度镉使叶片失绿 ,叶片N、P、K含量增加 ;硒可减轻镉对水稻幼苗的毒害 ,表现为减轻镉对苗长度的抑制、提高叶绿素含量、苗鲜重 ,以及使叶片N含量增加、K含量降低等 .硒可提高叶片P的含量 ,硒和镉的互作效应影响叶片P的含量     

籼型三系杂交水稻株高的发育遗传分析

采用数量性状的加性—显性发育遗传模型分析了按 NC 交配设计的两套籼型三系杂交水稻株高的发育遗传规律 .结果表明 ,株高在不同发育阶段均受加性效应和显性效应的共同控制 ,分蘖期以加性效应为主 ,抽穗期以显性效应为主 .随着发育进程的推进 ,抽穗期的杂种优势最强 .另外 ,株高在整个发育时期易受基因型与环境互作效应的影响 ,该效应在一定程度上影响株高性状的表现     

Harm of Offgas from Wastewater Treatment Facilities and Its Controlling

Offgas problem of wastewater treatment systems is becoming an increasing problem, as the public's living standard and awareness of environmental issues increases. This article puts forward the necessity of cleaning the offgas from wastewater treatment facility, introduces the harms of odorous portion of offgas to public and concrete construction, brings forward the main object of offgas emitted from wastewater treatment plant. The way of restraining the generation and escaping of gases, the containment structure of facilities and decontaminate of offgas after it emit, and the virtues and shortcomings of each method are discussed in this article to give advices for the selection of controlling ways.     

Magnetoresistive Effect of Diamond Films

On the basis of F S thin theory and taking the scattering of lattice vibration and impurities into account, the conductivity and the magnetoresistance of p type semiconducting diamond films (strip sample) are derived in spherical energy band and parallel connection model for light hold band,heavy hole band and split off band of diamond by solving the Boltzmann equation in the relaxation time approximation,. The relations between the magnetoresistance and thickness of diamond films,magnetic field strength and mobility are given. The magnetoresistance is influenced differently by light hole band,heavy-hole band and split off band. The deviation of the magnetoresistance is small between thick diamond films and lump. Magnetoresistive effects relate closely with temperature,magnetic filed strength and mobility.     

The Quantitative Analysis of the Fatalness of the Methane Explosion in Coal Mines

If the quantitative index of the methane explosion in coal mines before exploding can be calculated, the measure will be take to prevent the methane explosion. The methods using in the analysis of the fatalness of the methane explosion are almost qualitative analysis. In this paper the quantitative analysis of the fatalness of the methane explosion in coal mines is achieved on the basis of the research of the essence event probability with the method of fault tree analysis using the micro-computer. The practical example of the quantitative analysis of the fatalness of the methane explosion in coal mines is given.     

Research Status of Dyeing Wastewater Treatment

Wastewater of dyeing industry has complicated constituents, and usually contains many kinds of dyes. It has deep tinct and strong toxicity, and it is difficult to decompose it. Its pH value fluctuates widely, and it has high concentration and large water quantity.The development status of the dyeing wastewater treatment inside and outside China is introduced, and the physics method, chemical method, physical chemistry method and biological method are stated in detail. The applicable conditions and treating effect of the four methods are listed and the advantages and disadvantages are concluded.     

An Economic Explanation of the Engineering Quality Risk and Risk Prevention

Each party in engineering project faces different quality risk in different stage,and each adopts different management decision against quality risk.Firstly,from the new classic economics angle,an economic explanation to the management decision against engineering quality risk according to the different benefits for contractor and owner.The owner and contractor of a construction project,the government and projection legal person find their economic ties in an asymmetric information supply,or in an imperfectly assigned contract.The rules and characteristics of entrust-agency relation are analyzed from the economics and information economics angle,an economic explanation of engineering quality risk is given according to entrust-agency relation.The measures of controlling quality risk have been put forward from the angle of the economics.