昆明不同亚气候带烤烟感官评吸质量与糖类及其相关指标的关系

为揭示不同亚气候带烟叶质量的变化规律,以昆明3个主要亚气候带(北亚热带、暖温带、中温带)种植的K326、云烟87、云烟99、NC102、NC297等5个烤烟品种为材料,分析了烤后中部烟叶糖类物质及其相关指标和感官质量的相互关系.结果表明:感官评吸分值及所测糖类物质及其相关指标均以在暖温带最高、北亚热带次之、中温带最低;评吸总分与各种指标均呈正相关,其中与淀粉、总糖、糖氮比呈显著正相关,与两糖差呈极显著正相关.     

晋麦47幼苗叶片水分胁迫差异表达蛋白的鉴定与分析

为了从蛋白质水平进一步揭示小麦抗旱机理,以旱地小麦品种晋麦47为试验材料,采用蛋白质双向电泳技术,结合质谱鉴定技术,研究幼苗在正常供水与水分胁迫(-0.5 MPa的PEG-6000溶液处理48h)后叶片蛋白质组表达谱间的差异,分析水分胁迫差异表达的蛋白种类及其功能.研究发现,在正常供水和水分胁迫处理两种条件下,幼苗叶片蛋白的双向电泳图谱均可重复检测到850个蛋白点;31个蛋白点在两种供水水平间表达量存在显著差异,其中17个蛋白点在水分胁迫处理下表达量明显增加,14个蛋白点表达量明显减少.对这些蛋白点进行肽指纹图谱分析,并利用Mascot软件在NCBInr数据库搜索,最终有26个蛋白点被成功鉴定;其中,未知功能蛋白7个;已知功能蛋白19个,主要涉及能量、代谢、抗病与防御、细胞结构、转录与翻译以及信号转导等功能类别.结果表明,小麦体内的多个代谢及调控途径参与了对干旱的应激反应.最后,本文还对鉴定到的差异蛋白点与水分胁迫的关系进行了分析和讨论.     

Application of and correlation among antioxidant and antiradical assays for characterizing antioxidant capacity of berries

Various antioxidant assays are used to characterize fruits consumed fresh or processed although an easy, reliable and cheap assay is not available to follow the changes in antioxidant power. This study tested the applicability of a recently developed antioxidant measuring system, Water-soluble (ACW) and Lipid-soluble (ACL) Antioxidant Capacity, using the PHOTOCHEM^® instrument. Initially, 13 cultivars of 4 berry species were assayed with Photochem and compared with widely used methods based on single electron transfer reactions like ferric reducing ability of plasma, total polyphenolic content (TPC) and 2,2′-diphenyl-1-picrylhydrazyl radical-scavenging capacity. Assays based on hydrogen atom transfer reactions were also carried out including total radical-scavenger capacity (TRSC). The correlations among the results were all significant at P ≤ 0.05, except the one between TPC and ACW. The overall antioxidant capacity ranked on the order of strawberry < red currant < raspberry < black currant. However, significant differences could have been detected among genotypes and antioxidants assays used for the analysis. The Photochem instrument is not suitable for easy, cheap and high-throughput routine analysis of berry antioxidant capacity and hence is not preferable to other assays.     

Proteome profiling of the compatible interaction between wheat and stripe rust

Over the last decade, comparative molecular profiling studies between compatible and incompatible plant-pathogen interactions have shown that susceptible response of the host to a pathogen requires factors that promote disease development. In this study, we examined proteome profiles during a compatible interaction between wheat and stripe rust. A 2D-LC system (ProteomeLab PF2D) was used for protein separation and to compare the proteome from infected and control samples. More than 700 protein peaks at each time point were compared between pathogen- and mock-inoculated samples. Selected proteins, with significant differences in abundance were identified by nanoLC-ESI- MS/MS and generated spectra were searched against the wheat protein databases from UniProt, and NCBI and the Puccinia database from The Broad Institute. In total, the identified proteins comprised of 62 % wheat and 38 % Pst proteins. All identified proteins were searched by bioinformatics-based algorithms to detect their subcellular localization and signal peptide motifs which have the potential to catch the candidate effector proteins. The wheat proteins were classified based on their function. Although a compatible interaction, many wheat proteins, such as antioxidants, PRs and cold-responsive proteins, are implicated in defense and stress tolerance. On the pathogen side, 64 proteins were identified, and included some important pathogenicity proteins that can play role in pathogen virulence and suppress the host defense. In addition, we discovered that nine proteins have a signal sequence and three of the hypothetical fungal proteins, PGTG_11681T0, PGTG_07231T0 and CBH50687.1, have been tentatively identified as candidate effectors.     

一类非线性klein－Gorden方程与热传导方程耦合组的Cauchy问题的整体经典解的存在唯一性

本文讨论非线性ｋｌｅｉｎ－Ｇｏｒｄｅｎ方程与非线性热传导方程耦合组的Ｃａｕｃｈｙ问题，对初值和空间维数及非线项加以适当的限制，利用能量估计和衰减估计相结合的方法，在Ｓｏｂｏｌｅｖ空间框架下，得到了整体经典解的存在唯一性     

钢筋混凝土框架柱计算长度设计方法研究

首先采用直接由材料本构关系形成单元M-N-Φ本构矩阵的方法推导出钢筋混凝土构件的单元刚度矩阵，编制了可以同时考虑材料和几何双重大量线性影响的有限元程序，与独立柱和框架柱的试验结果相比，计算结果吻合较好；然后，采用该分析程序对一榀二跨七层的规则框架算例进行分析，计算了各柱单元控制截面的二阶弯矩，并分析了二阶弯矩随各影响因素的变化规律；最后，以现行设计规范中标准柱偏心距增大系数的原型公式在本研究程序条件下修正公式为基点，分析了所选算例各柱单元的计算长度随各影响因素的变化规律。     

轮虫有性生殖的研究

比较全面地概括了近年来国内外对轮虫有性生殖研究的最新进展，重点介绍影响轮虫混交雌体产生的外源性因素和内源性因素。影响轮虫混交雌体产生的外源性因素主要有种群密度、光照周期，食物情况，生育酚，培养液的温度、盐度、pH值，内源性因素主要有遗传因素、母体年龄和轮虫弧雌生殖的累计世代数。     

南方现代化温室黄瓜夏季蒸腾研究

 温室作物蒸腾不仅直接影响到温室内空气温湿度 ,而且是现代温室中确定合理的作物肥水灌溉控制目标的主要依据。研究采用Penman Monteith方程模拟南方现代化温室黄瓜在夏季高温高湿条件下的蒸腾速率 ,并通过冠层微气候和蒸腾速率观测 ,分析了影响蒸腾的主要温室环境因素。结果表明 ,Penman Monteith方程模拟黄瓜夏季蒸腾速率结果较为可靠且模型具有一定的鲁棒性。温室黄瓜夏季蒸腾速率随辐射强度 (净辐射 )和空气饱和水汽压差 (VPD)的增加而线性增大 ,但蒸腾速率日最大值出现时间较净辐射滞后而与VPD较为一致。     

草本植物纤维生物提取菌种资源整理与整合

本文按照国家微生物资源标准化整理与整合要求,从中国农业科学院麻类研究所保存的微生物资源中随机选择802份菌种资源进行规范化描述,累计整合信息数据64574项,修复、补充信息数据27767项,经过分类鉴定证明,这些菌种资源涉及22属38种;同时,经过活化、提纯、复壮并保藏菌株615份,涉及20属31种.相关信息和实物整合到中国农业微生物菌种保藏管理中心（ACCC）保藏.     

NAA和2，4ˉD对东方百合组织培养的影响

借助正交回归多项式建立数学模型的方法,研究了NAA对东方百合“蒂伯”（Ti ber）鳞片 诱导小鳞茎及2,4ˉD对小叶片诱导愈伤组织的影响,以探讨东方百合组织培养中NAA及 2,4ˉD 的用量范围及最佳用量。结果表明, NAA诱导离体鳞片外植体产生小鳞茎的用量范围为 0.15～ 0.9 mg·L -1 ,最佳用量为0.69 mg·L -1 ; 2,4ˉD诱导叶片形成愈伤组织的最佳用量为3.2 mg· L -1 。