全文获取类型
收费全文 | 85048篇 |
免费 | 3830篇 |
国内免费 | 9332篇 |
专业分类
林业 | 9321篇 |
农学 | 12204篇 |
基础科学 | 7104篇 |
13196篇 | |
综合类 | 28162篇 |
农作物 | 4651篇 |
水产渔业 | 2944篇 |
畜牧兽医 | 11709篇 |
园艺 | 3525篇 |
植物保护 | 5394篇 |
出版年
2024年 | 258篇 |
2023年 | 988篇 |
2022年 | 2389篇 |
2021年 | 3342篇 |
2020年 | 2915篇 |
2019年 | 2958篇 |
2018年 | 2120篇 |
2017年 | 3013篇 |
2016年 | 2787篇 |
2015年 | 3901篇 |
2014年 | 3772篇 |
2013年 | 4517篇 |
2012年 | 5580篇 |
2011年 | 5967篇 |
2010年 | 5930篇 |
2009年 | 5430篇 |
2008年 | 5003篇 |
2007年 | 5144篇 |
2006年 | 4802篇 |
2005年 | 4233篇 |
2004年 | 2058篇 |
2003年 | 1954篇 |
2002年 | 1706篇 |
2001年 | 1700篇 |
2000年 | 1754篇 |
1999年 | 1966篇 |
1998年 | 1716篇 |
1997年 | 1548篇 |
1996年 | 1410篇 |
1995年 | 1270篇 |
1994年 | 1087篇 |
1993年 | 1117篇 |
1992年 | 875篇 |
1991年 | 740篇 |
1990年 | 624篇 |
1989年 | 502篇 |
1988年 | 385篇 |
1987年 | 229篇 |
1986年 | 130篇 |
1985年 | 67篇 |
1984年 | 45篇 |
1983年 | 40篇 |
1982年 | 49篇 |
1981年 | 48篇 |
1980年 | 32篇 |
1979年 | 14篇 |
1965年 | 15篇 |
1964年 | 11篇 |
1963年 | 12篇 |
1956年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
871.
试验旨在研究Toll 样受体(Toll-like receptors,TLRs)在水貂抗病毒免疫中的作用机制,并为抗病育种积累可供选择的基因素材。本试验以雪貂TLRs基因为参考序列设计4对引物对水貂TLR4、TLR6、TLR7及TLR8进行分子克隆,并对所得序列进行生物信息学分析,进一步利用半定量RT-PCR技术分析TLR4和TLR7基因mRNA在不同年龄水貂各组织中的表达情况。结果表明,水貂TLRs与食肉目动物(雪貂、北极熊、大熊猫、海象、海豹、犬、老虎和猫)具有较高的同源性,在系统发育树中距离最近;组织表达分析表明TLR4和TLR7在检测的组织中广泛表达且表达量存在差异,相比成年水貂,仔貂各组织中TLR4或TLR7基因表达量更高。 相似文献
872.
873.
为给规模猪场猪伪狂犬病(PR)免疫程序制定提供参考,在贵州省某规模猪场选取3组不同母源抗体背景仔猪群,按照不同的免疫程序进行PR疫苗免疫,然后设置不同的检测时间点,采集猪血清采用ELISA方法进行gE和gB抗体跟踪检测,初步探索仔猪群免疫抗体消长规律。结果显示:在母源gE和gB抗体均为阴性背景下,1日龄初生仔猪活疫苗滴鼻免疫对免疫抗体水平没有影响,15日龄基因缺失疫苗注射免疫后,免疫抗体水平逐渐升高,45日龄基因缺失疫苗加强免疫1个月后,免疫抗体水平达到顶峰,此后逐渐下降,到120日龄时,仍有50%的有效保护率;在gE母源抗体阴性背景下,产前40 d免疫母猪,55日龄进行基因缺失疫苗注射免疫后,仔猪免疫抗体水平持续升高,90日龄时再用基因缺失疫苗加强注射免疫1次,至120日龄免疫抗体水平达到顶峰,150日龄后开始下降,到185日龄时,仍有50%的有效保护率;在母源gE抗体阳性背景下,疫苗免疫不能使仔猪产生有效的免疫保护抗体。结果表明,仔猪母源抗体背景对于仔猪免疫程序的制定具有较大影响,合理的免疫程序要结合母源抗体和免疫抗体检测结果合理制定,并要及时淘汰gE阳性种猪。 相似文献
874.
875.
876.
Xuerui Yao Hao Jiang Ying‐Hua Li Qingshan Gao Yong Nan Xu Nam‐Hyung Kim 《Animal Science Journal》2019,90(11):1417-1425
Kaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti‐inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging‐related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE‐treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency‐related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE‐treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function. 相似文献
877.
878.
Xian Tan Jia Li Yali Li Jianzhong Li Qingping Wang Lin Fang Xueqin Ding Pengfei Huang Huansheng Yang Yulong Yin 《Journal of animal physiology and animal nutrition》2019,103(5):1503-1511
This study was conducted to test the hypothesis that dietary supplementation with anti‐E. coli, chicken egg yolk immunoglobulins (IgY), may affect early weaned piglet (EWP) intestinal functions and enteric micro‐organisms. One hundred and forty‐eight ([Landrace × Yorkshire] × Duroc) piglets, weaned at age day 21, were randomly assigned to receive one of three diets for 14 days. Treatment group one (control group) was fed the base diet. Treatment group two (antibiotics group) was fed the base diet which was supplemented with 100 ppm colistin sulphate and 15 ppm enramycin; treatment group three (IgY group) was fed the base diet which was supplemented with 500 mg/kg anti‐E. coli IgY. The study evaluated the effects on EWPs of IgY on growth, serum biochemical, inflammatory profiles and also digestion content intestinal bacterial populations. Results showed no significant difference in diarrhoea rates between IgY‐fed EWPs and antibiotic‐treated EWPs. Serum biochemical analysis showed that EWPs fed an IgY‐containing diet had both lower (p < 0.05) cholesterol and low‐density lipoprotein compared to antibiotic‐treated EWPs. Escherichia coli populations measured in IgY‐fed EWP ileal contents, compared to the control group, were significantly reduced (p < 0.05). Enterococcus, Lactobacillus, Clostridium and Bifidobacterium populations were unaffected by the IgY treatment. Larger (p < 0.05) Enterococcus populations and lower (p < 0.05) expression levels of heat‐stable enterotoxin b (STb) were observed in IgY‐fed EWP caecal digesta compared to the control group. Enteric Lactobacillus significantly decreased (p < 0.05) in EWPs fed antibiotics while it was unaffected by IgY treatment. Dietary supplementation with anti‐E. coli IgY has the potential to suppress enteric E. coli growth, but not Lactobacillus, Clostridium and Bifidobacterium. This promotes and maintains a healthy EWP intestinal environment. These findings suggest that IgY may be used as an alternative to antibiotics in EWP diets. 相似文献
879.
Zirui Zhou Jie Zhang Xianlian Zhang Shilan Mo Xian Tan Lixia Wang Jianzhong Li Yali Li Xueqin Ding Xianyong Liu Xingqun Ma Huansheng Yang Yulong Yin 《Journal of animal physiology and animal nutrition》2019,103(5):1530-1537
Weaning process widely affects the small intestinal structure and function in piglets, while the responses of large intestine to weaning stress are still obscure. The purpose of this study was to determine the developmental changes (i.e., short chain fatty acids (SCFAs) concentrations, growth parameters, crypt‐related indices and antioxidant capacity) in colon of piglet during weaning. Forty piglets were weaned at day 21 and euthanized to collect colonic tissues and digesta samples on day 0, 1, 3, 7 and 14 post‐weaning (n = 8). Piglet growth performance was improved (p < .001) on day 7 and 14 post‐weaning. The concentrations of acetate, propionate, butyrate, valerate, isobutyrate, isovalerate and total SCFAs were higher (p < .001) during the late post‐weaning period. The mRNA abundances of SCFAs transporters were greater (p < .001) on day 7 and 14. The absolute and relative weights, absolute length and perimeter of colon were greater (p < .001) on day 7 and 14. Similarly, post‐weaning increases (p < .001) in colonic crypt depth and Ki67 positive cells numbers per crypt were observed during the same period. Colonic crypt fission indices decreased (p < .01), while total crypt numbers increased (p < .001) on day 14 after weaning. Moreover, total SCFAs concentration was significantly associated with colonic growth parameters and Ki67 cells/crypt (p < .001). In addition, catalase content was decreased on day 3, 7, and 14, whereas, the concentrations of total superoxide dismutase (T‐SOD) and manganese‐containing superoxide dismutase (MnSOD) were higher (p < .05) on day 1 and 3 post‐weaning. These results showed that weaning process has a significant effect on colonic growth and development, which might be associated with the change of SCFAs concentrations in colon. 相似文献
880.
Xiaodong Zhang Liang Zhang Jinnan Shang Qiangqiang Tao Mi Tian Yingchun Ma Yiliang Xu Yueyun Ding Rong Zhou Kui Li Zongjun Yin 《Reproduction in domestic animals》2019,54(7):1018-1025
The aim of this study was to explore the expression difference of miRNAs and mRNAs between the follicular phase (FP) and luteal phase (LP) in porcine ovaries and provide a theoretical basis for the research on mammalian reproductive regulation. RNA‐Seq and miRNA‐Seq were used to identify differentially expressed genes (DEGs) and miRNAs (DEMs) between the FP and LP in ovaries of six sows (3‐year‐old Yorkshire pigs with similar weights and same parities). Bioinformatic analysis was used to screen potential genes and miRNAs related to porcine ovarian function. Real‐time qualitative PCR was used to validate the sequencing results. RNA‐Seq results showed that 3,078 genes were up‐regulated, and 1,444 genes were down‐regulated in the LP compared with the FP, and DEGs were significantly enriched in 242 Gene Ontology (GO) terms and 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. miRNA‐Seq identified 112 DEMs, of which 25 were up‐regulated and 87 were down‐regulated in the LP compared with the FP. We obtained 186 intersection genes (IGs) between the 4,522 DEGs and 2,444 target genes predicted from the 112 DEMs. After constructing a miRNA‐gene‐pathway network, we identified key miRNAs and genes including miR‐17‐3p, miR‐214, miR‐221‐5p, miR‐125b, FGF1, YWHAG, YWHAZ, FDFT1 and DHCR24, which are enriched in Hippo and PI3K‐Akt signalling pathways, and various metabolic pathways. These results indicate that these key genes and miRNAs may play important roles in the developmental transition from FP to LP in porcine ovaries and represent candidate targets for further study. 相似文献