The effect of lactic acid bacterial starter culture and chemical additives on wilted rice straw silage

Lactic acid bacteria (LAB) are suitable for rice straw silage fermentation, but have been studied rarely, and rice straw as raw material for ensiling is difficult because of its disadvantages, such as low nutrition for microbial activities and low abundances of natural populations of LAB. So we investigated the effect of application of LAB and chemical additives on the fermentation quality and microbial community of wilted rice straw silage. Treatment with chemical additives increased the concentrations of crude protein (CP), water soluble carbohydrate (WSC), acetic acid and lactic acid, reduced the concentrations of acid detergent fiber (ADF) and neutral detergent fiber (NDF), but did not effectively inhibit the growth of spoilage organisms. Inoculation with LABs did not improve the nutritional value of the silage because of poor growth of LABs in wilted rice straw. Inoculation with LAB and addition of chemical materials improved the quality of silage similar to the effects of addition of chemical materials alone. Growth of aerobic and facultatively anaerobic bacteria was inhibited by this mixed treatment and the LAB gradually dominated the microbial community. In summary, the fermentation quality of wilted rice straw silage had improved by addition of LAB and chemical materials.     

苹果果实表皮总蛋白提取及双向电泳方法的优化

以苹果果实表皮为试材,采用3种方法提取果实表皮总蛋白,并对双向电泳(2-DE)操作体系进行优化。结果表明:改良酚提法适于苹果果实表皮总蛋白的提取,优化后的2-DE分离体系为预制IPG胶条18cm pH 4~7,上样量600μg,改进后的等电聚焦程序,浓度16%的分离胶,适于苹果果实表皮总蛋白的2-DE分离。     

Safety of an attenuated West Nile virus vaccine, live Flavivirus chimera in horses

REASON FOR PERFORMING STUDY: West Nile virus (WNV) infection is endemic and able to cause disease in naive hosts. It is necessary therefore to evaluate the safety of new vaccines. OBJECTIVES: To establish: 1) the safety of a modified live Flavivirus/West Nile virus (WN-FV) chimera by administration of an overdose and testing for shed of vaccine virus and spread to uninoculated sentinel horses; 2) that this vaccine did not become pathogenic once passaged in horses; and 3) vaccine safety under field conditions. METHODS: There were 3 protocols: 1) In the overdose/shed and spread study, horses were vaccinated with a 100x immunogenicity overdose of WN-FV chimera vaccine and housed with sentinel horses. 2) A reversion to virulence study, where horses were vaccinated with a 20x immunogenicity overdose of WN-FV chimera vaccine. Horses in both studies were evaluated for abnormal health conditions and samples obtained to detect virus, seroconversion and dissemination into tissues. 3) In a field safety test 919 healthy horses of various ages, breeds and sex were used. RESULTS: Vaccination did not result in site or systemic reactions in either experimental or field-injected horses. There was no shed of vaccine virus, no detection of vaccine virus into tissue and no reversion to virulence with passage. CONCLUSIONS: WN-FV chimera vaccine is safe to use in horses with no evidence of ill effects from very high doses of vaccine. There was no evidence of reversion to virulence. In addition, administration of this vaccine to several hundred horses that may have been previously exposed to WNV or WNV vaccine resulted in no untoward reactions. POTENTIAL RELEVANCE: These studies establish that this live attenuated Flavivirus chimera is safe to use for immunoprophylaxis against WNV disease in horses.     

Effects of Low- Intensity Ultrasound Treatment on Excess Sludge

In this study the influence of the low-intensity ultrasound treatment on excess sludge has been observed.The oxygen uptake rate(OUR),soluble chemical oxygen demand(SCOD),soluble total nitrogen(TN) and soluble total phosphorus(TP) were examined for three power levels of 0.03 W/mL, 0.05 W/mL and 0.1 W/mL as well as two time series of 0~5 min and 0~30 min.It showed that there were two stages of ultrasonic treatment: the first stage took place within the flocs in four minutes,in which SCOD,TN,TP were dissolved from flocs.In the second stage,the microbial activities have been strengthened,as well as OUR,TN,TP have increased greatly.     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

CALCULATION OF TNE END-EFFECT FACTOR OF SYNCHRONOUS MOTOR WITH CYLINDRICAL SOLID ROTOR

This paper presents the end-effect factor of Synchronous motor with cylindricalsolid rotor offered by analysing the 3-D electromagnetic field and using the Poynting vector.To ap-ply it in an actual calculation,the end-effect factor is proved correct and applicable.     

Study on Application Model of Knowledge Expression

As a key resource, the knowledge management is becoming a research field interested by many researchers. In order to study the mathematics theory base of knowledge expression, in this paper, a knowledge expression model is given. In this model the knowledge system is expressed by multi-tuple, the single knowledge object is expressed by the tuple, a structural expression of knowledge is given using token as example and the application of the model in city water supply pipe network is presented in this paper.     

Real-time Performance of Linux System

The standard Linux is a typical time-sharing system, it has a poor real-time performance. But according to the applications of the Linux OS are expanding on real-time domain, it becomes necessary to enhance the performance of the Linux system. This paper analyses the schedule policies and schedule algorithms of the Linux kernel, and discusses several different kinds of methods about enhancing real-time performance of the Linux system from the total Linux system. They authors summarizes the current research's developments of the Linux system.     

小黄鱼热泵干燥工艺及设备选型分析

研究了不同热泵干燥温度下小黄鱼的干燥特性,并对其干燥品质进行分析。结果表明在干燥温度55℃、铺放密度为6.48 kg/m2的条件下干燥时间为13.5 h,干燥品质最优。在综合考虑能耗、企业加工条件和产品品质的前提下,将生产工艺参数确定为:干燥温度60℃、铺放密度为6~7 kg/m2,干燥时间为10~12 h。根据企业日处理量5.5~6.0 t的加工要求,通过对产能核算及加工成本计算,确定设备选型方案为:1台GHRH-510型热泵干燥机与2台GHRH-340型热泵干燥机,该方案投资回报期短,社会效益明显。     

水稻田间育秧精密播种机设计与试验

为提高田间育秧播种机的播种合格率和播种稳定均匀性,设计了一种适用于南方水稻田间育秧的精密播种机。在深入分析螺旋勺轮式播种器的播种原理,以及播种器行走控制系统的基础上,采用正交试验方法,研究了螺旋勺轮的凹槽深度、螺旋升角、播种器行走速度对播种合格率和空穴率的影响规律,得出杂交稻播种合格率和空穴率影响因素的最佳参数组合:凹槽深度h为3 mm、螺旋升角α为81.73°、播种器行走速度v为0.35 m/s。试验表明,采用闭环控制系统后的播种机播种杂交稻2~6粒/格合格率由87.14%提升到93.21%,常规稻3~8粒/格的播种合格率为92.14%,播种性能满足常规稻和杂交稻精密播种育秧的技术要求。