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11.
猪圆环病毒2型(PCV2)是猪圆环病毒相关疾病(PCVAD)的必要病原,对养猪生产构成了严重威胁,但当前缺乏有效的防治手段.为了探索治疗PCVAD的新途径,分别针对PCV2的病毒复制相关蛋白基因(rep)与衣壳蛋白基因(cap),构建了PCV2特异的siRNA表达质粒(pGensil-R259、pGensil-C297和pGensilC490)和PCV2非特异的阴性对照质粒(pGensiI-SCR).在脂质体介导下,将构建的siRNA表达质粒与阴性对照质粒转染PK-15细胞,转染质粒20 h后感染PCV2.结果质粒表达的特异siRNA抑制了PCV2 DNA的合成及Rep和Cap蛋白的表达,使病毒滴度(TCID50)显著下降;针对靶基因不同位点的siRNA的抑制效果不同,病毒感染后36 h内,siRNA对PCV2的抑制作用最强,随后有所下降,但直至感染后60 h仍然能够显著抑制PCV2复制.实验结果提示质粒表达的特异siRNA能够显著地抑制PCV2的增殖,但这种抑制功能与作用时间呈负相关. 相似文献
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采用超声波及冰浴法对咪松酚乳膏进行前处理,以反相高效液相色谱法同时测定了咪松酚乳膏中硝酸咪康唑、醋酸地塞米松和丹皮酚的含量.硝酸咪康唑线性范围为40.5~648.0 μg/mL,r=0.999 1;醋酸地塞米松线性范围为3.05~48.8 μg/mL, r=0.999 8;丹皮酚线性范围为20.5~328.0 μg/mL, r=0.999 4;硝酸咪康唑、醋酸地塞米松及丹皮酚的平均回收率分别为:100.44±1.25(%), 98.46±1.04(%) 和98.24±1.29(%). 相似文献
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A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide 总被引:2,自引:0,他引:2
Wang JL Liu MQ Han J Chen WZ Cong W Cheng G Gao YH Lu YG Chen JL Zuo XP Yan WY Zheng ZX 《Veterinary microbiology》2007,125(3-4):224-231
The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide. 相似文献
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The effects of short-term nutrient additions (NPKμ, 10 g·m- 2) and disturbances on the species diversity, functional traits, soil properties, and community assembly mechanisms of the herbaceous community in the Urat desert steppe were examined. A mixed linear model, principal component analysis, and null model methods were utilized and both disturbance and interaction treatments were found to significantly reduce species richness and β diversity. Short-term nutrient additions significantly reduced soil pH but increased soil electrical conductivity. Six functional traits of the dominant species (i.e. Stipa glareosa, Peganum harmala, Salsola collina, Corispermum mongolicum) and six community-weighted mean significant changes under the disturbance and nutrient addition treatments were identified. The disturbance and nutrient additions promoted the transformation of the dominant species in the community from conservative strategies to acquisitive strategies. The results of the two null models revealed that community assembly under the control tended to be a stochastic process, while the disturbance and interaction treatments tended to be deterministic processes, and that of the nutrient addition treatments tended to be weaker deterministic processes. Plant communities in the desert steppe responded and adapted to the nutrient additions and disturbances through changes in the dominant species and their crucial functional traits. © 2023 Chinese Academy of Sciences. All rights reserved. 相似文献
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Discussion of the Application and Foreground of Falling Water Aeration with the Process of Sewage Treatment
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The engineering design of sewage treatment plant for the Chongqing city, P. R. China is analyzed in this paper. Considering the situation of China and other projects, the characteristics of falling water reactor for the environment protection project and economy of investment is introduced ; the application and foreground of falling water aeration in the process of sewage treatment is discussed, such as Oxidation - ditch, SBR, and so on. The conclusion indicated that the falling water aeration can be designed and used as an independent aeration, and, it is an upgraded technology of sewage treatment which is adaptive for city water environment control in middle and small towns in China. The falling water aeration may reduce running cost of the sewage treatment plant, is adaptive for foothill and the mountainous areas, and reduces project investment and environments damage. 相似文献
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CHEN Zuo chang 《保鲜与加工》2002,(2):93-97
With social development, the environment has become one of the major topics in human life. Although the construction project may improve economy and environment, it also may exert negative impact on environment. Cost, schedule and quality are three important objectives of construction project and on the objective of environment have not been focused. The author of this paper reiterated the importance of environmental control for the construction project and key areas to be focused on during the whole process of construction project. 相似文献
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陈正洪 《华中农业大学学报》1993,12(4):369-375
本文根据主导因子及综合分区原理,对与我省森林火灾有关的主要气候要素进行单项及综合区划,共得到3级共8个林火气候区,其中,重、中等火灾区各占1/7,轻火灾区占5/7。 相似文献