中间砧对“茂谷橘橙”树体和果实品质的影响

以蕉柑、芦柑和改良橙作为茂谷橘橙的中间砧，对各中间砧组合的亲和性、树体及枝梢生长表现、产量和果实品质等进行比较研究，结果表明，3种中间砧与接穗的亲和性无显著差异，均表现为亲和力强；改良橙作中间砧的冠径，春梢、夏梢、秋梢的生长量和早期产量均显著地低于蕉柑、芦柑的。高接5年后，3种中间砧对产量的影响无显著差异。3种中间砧对茂谷橘橙果实外观品质和内部品质的影响都不明显。     

旋耕刀正切刃设计方法的研究

论述了旋耕刀正切刃的设计，推导了正切丸弯折方程，讨论了正切刃滑切角的计算，应用偏心圆方法设计了正切刃，并对有关参数进行了分析。     

机组段运转特性曲线的绘制及其应用

机组段是水电站运行的基本单元。从运行的需要出发,采用机组段运转特性曲线来指导运行,比用水轮机运转特性曲线方便。本文介绍机组段运转特性曲线的绘制方法及其在水电站运行中的作用。     

深松耕作对土壤水分物理特性及作物生长的影响

通过对两种土壤深松耕作及传统耕作的对比试验研究，表明深松耕作可有效打破犁底层，使土壤容重降低0．1t／m3，孔隙度增大3．6％～4．0％，110cm上体内可多蓄纳雨水15．2～17．5mm。对粘壤上深松耕作可提高土壤的入渗能力，而对粉砂壤土则没有提高。同传统耕作相比，深松耕作可明显提高夏玉米的根长、根深及根量，为有效利用土壤水分及养分创造了条件．可使夏玉米产量提高5．7％～11．3％。     

基于超磁致伸缩转换器的流体控制阀及其技术

介绍了超磁致伸缩材料在流体控制阀中的应用研究现状，详细分析了使用GMA时要解决的热补偿、微位移放大和非线性控制等几项关键技术。提出了两种基于GMA的两级电液伺服阀实现方案。     

棱锥型孔排种部件的研究

根据地膜覆盖精量播种玉米的要求，研制了棱锥型孔排种部件，介绍了它的工作原理及基本参数，经台架试验得出了该排种器结构参数的较优设计方案。在较优设计参数下，棱锥型孔排种圆盘的线速度ｖ＝０．８０７ｍ／ｓ时，单粒率为９３．６７％，空穴率为１．６７％，其指标达到了机械式膜上玉米精量播种的先进水平，为地膜覆盖播种玉米提供了一种机械式精量排种器。     

水牛脾切除术的研究

近3年来成功地作了20例水牛的脾切除术,没有1例因手术而招致死亡。成功地探索出适合于水牛脾切除术的麻醉方法、切口部位和大小、手术操作方法。     

A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988^T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.     

第三次全国农作物种质资源调查与收集行动湖南茶树专项总结报告

2015年10月至2018年12月,项目组参与第三次全国农作物种质资源调查与收集行动,对湖南省茶树种质资源进行普查、调查和征集、收集,共普查、调查和征集、收集茶树种质资源165份,涉及45个农业县(市、区),范围为东经109.176821°~114.005865°、北纬24.943720°~29.819774°、海拔31.33~1469.00m,并对湖南茶树种质资源的保护和合理开发利用提出了建议。