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21.
22.
Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31 总被引:4,自引:0,他引:4
Specific oligonucleotide primers, designed for the sequences of known plant disease resistance genes, were used to amplify resistance gene analogues (RGAs) from wheat genomic DNA. This method was applied in a bulked segregant analysis to screen for the RGA markers linked to the powdery mildew resistance gene Pm31, introgressed into common wheat from wild emmer. Two RGA markers (RGA200 and RGA390) were found to be closely linked to Pm31 and completely co‐segregating with the marker allele of Xpsp3029 linked to Pm31, with a genetic distance of 0.6 cM. These two RGA markers were then integrated into the formerly established microsatellite map of Pm31 region. The result showed the effectiveness of the RGA approach for developing molecular markers linked to disease resistance genes and demonstrated the efficiency of denaturing polyacrylamide‐gel electrophoresis for detecting polymerase chain reaction polymorphism. 相似文献
23.
突扩能够使局部范围内水流紊动剧烈,可以作为水利工程中消能工的一种重要方式.为了探讨突扩比对下游流体水力特性的影响规律,基于RNG k-ε紊流模型和PISO压力-速度耦合算法,采用非均匀结构网格技术,建立了三维紊流数学模型,对突扩比分别为1000,0833,0.667,0500,0333等5种空间水跃进行了多工况数值模拟,结合试验数据,计算结果量纲一化,讨论了不同突扩比大小对下游水力性能的影响,并分析了下游流速、紊动能及消能率等随突扩比的变化规律.数值计算结果表明:突扩比对下游流速、紊动能及消能率影响显著,突扩比越小,下游流速变化越显著,主流在空间内扩散愈迅速,流态转变越明显;随着突扩比的减小,紊动应力愈小,紊动能量愈小,而消能率越大;突扩水跃较典型水跃消能效果明显增强,当突扩比小于0500时,消能率增大不再显著. 相似文献
24.
郑毅 《绿色中国(A版)》2001,(4):45-47
负债经营可给企业带来利益 ,但如使用不当会给企业带来不利影响 ,导致企业破产 ,当资产利润率大于负债利息率时 ,负债经营可使自有资金利润率扩大 ,但同时财务风险提高。林业企业在经营活动中应适度把握负债比例 ,并考虑 8个方面的问题 ,善于经营负债。 相似文献
25.
通过目测进行分类的方式很难准确界定矾根品种叶色。为建立一套更为科学的基于叶色表型的矾根品种分类体系,笔者利用色差仪对72个矾根品种共计432个样品的叶色进行测定,对获得的L、a、b 3个色度值进行聚类分析,将72个矾根品种分为褐色系、深绿色系、黄色系、灰绿色系、浅绿色系5类色系。对矾根品种各色系的L、a、b值进行相关性分析,发现叶片正面的L值与a值、a值与b值呈负相关,L值与b值呈正相关,但相关性不明显;叶片背面的L值与a值、a值与b值呈负相关,L值与b值呈显著正相关。从多重比较的结果中可以看出,矾根品种各色系之间的L、a、b色度值差异显著,尤其是各色系间叶正面的颜色差异较大,在矾根品种选育工作中,叶片正面的颜色将作为主要参考。 相似文献
26.
为了明确ABA对孕穗期低温下水稻结实率及叶片生理特性的调控效应,本试验在盆栽条件下,以2个水稻(Oryza sativa L.)品种‘龙粳11’(冷敏型)和‘龙稻5’(耐冷型)为材料,于孕穗期在人工气候室进行低温(15℃,持续1,2,3,4,5天)处理,并喷施不同浓度的脱落酸(ABA),研究低温条件下ABA对不同水稻结实率的调控效应,同时探讨了ABA和低温对水稻叶片膜透性、抗氧化酶等生理特性的影响。结果表明:低温条件下,‘龙稻5’的结实率变化不大,‘龙粳11’的结实率显著降低,20 mg/L的ABA能够有效缓解低温伤害,相对提高低温胁迫下水稻的结实率。叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性表现出先升高后下降的变化趋势,‘龙稻5’的抗氧化酶活性、可溶性糖和可溶性蛋白含量高于‘龙粳11’,但丙二醛(MDA)含量和相对电导率低于‘龙粳11’。ABA可提高冷敏品种的抗氧化酶活性、可溶性糖和可溶性蛋白的含量,同时可降低MDA含量和相对电导率。这说明ABA可以通过调控水稻抗氧化酶、可溶性糖等生理指标的活性及含量,进而提高冷敏水稻品种的耐冷性。 相似文献
27.
Weiguo?Cheng Wenxi?LuEmail author Xin?Xin Zheng?Zhang Haibo?Chu 《Paddy and Water Environment》2016,14(2):355-365
The existing irrigation schedule of most rice-growing areas in Jilin Province is not in full compliance with the Water Requirements Law of the rice; these discrepancies affect the yield of rice to some extent. Thus, research on rice water regulations and selection of an appropriate water production function model (WPFM) is very important. In this study, according to the water demand characteristics of rice, we used drought treatments separately during the tillering, jointing booting, flowering, and milky stages. Evapotranspiration and yield data were obtained using a test pit experiment and production measurements in 2011 and 2012, respectively. Five commonly used WPFMs (the Jensen, Blank, Minhas, Stewart, and Singh model) were selected for this study, and the water sensitivity index of each model was calculated based on the evapotranspiration, yield, and the model equations. The Jensen model was found to be the most suitable WPFM for this region; furthermore, a mathematical relationship between the water sensitivity index and days after rice planting was established. These results have important theoretical and practical implications for guidelines regarding rice irrigation in Jilin Province. 相似文献
28.
Faculty members from veterinary colleges that teach courses on food-borne, waterborne, and zoonotic diseases to non-veterinary students face unique challenges. The diversity of students' backgrounds, skills, and goals and the public nature of the science require some ingenuity in combining good science with the ability to communicate clearly in a variety of public forums. Drawing on pedagogical theory, the nature of the material, and the diverse abilities of the students, we have put together an effective teaching package for non-veterinary courses on these subjects, which includes a range of methods from outbreak investigations to town hall meetings and food diaries. 相似文献
29.
30.
Silvia P. Turner Tanja Maria Hess Kibby Treiber Erica B. Mello Bruno G. Souza Fernando Q. Almeida 《Journal of Equine Veterinary Science》2011,31(11):645-649
Diets high in concentrates and soluble carbohydrates are associated with reduced insulin sensitivity in horses. Exercise training could protect against diet-induced insulin resistance. The objective of this study was to determine the intensity of exercise training required to affect insulin sensitivity in stabled horses fed a diet high in concentrates but moderate in soluble carbohydrates. In all, 31 stabled horses underwent three different exercise regimens: turnout, light exercise, and moderate exercise, while being fed a diet containing 60% concentrate. Blood was sampled monthly and analyzed for insulin. Insulin sensitivity was assessed using basal insulin concentrations and calculated insulin sensitivity (reciprocal of the square root of insulin) and compared across months by analysis of variance with repeated measures. Insulin sensitivity (reciprocal of the square root of insulin) was higher during periods of moderate and light physical activity as compared with turnout. These results indicate that turnout alone may not be adequate to improve insulin sensitivity in horses fed high amounts of concentrate. 相似文献