马铃薯甲虫空间分布型及序贯抽样

马铃薯甲虫Leptinotarsa decemlineata Say是一种重要的害虫,为明确其在新疆的空间分布,及时采取有效的防控措施,在田间调查的基础上,采取随机取样和Iwao回归分析法对新疆乌鲁木齐地区的马铃薯甲虫成虫、卵块和幼虫的空间分布型、理论抽样数及幼虫的序贯抽样方法进行了研究。结果表明,马铃薯甲虫各虫态空间格局的聚块性指标均大于1,空间分布型为普通群聚型;对低龄幼虫进行序贯抽样,当防治阈值为50头/样方、置信水平为1.96时,防治上、下限方程分别为:d1=50n+81n和d0=50n-81n,当百株虫量达到756头以上时需要进行防治。     

农药中毒的原因分析及防治对策

为探讨农业生产过程中农药中毒的发生原因及规律,我们在枣阳市的吴店、南城、兴隆和鹿头等乡镇进行了调查和试验,为广大农民科学掌握使用农药的方法进行了有益探索,并研究制定了科学有效的预防对策.     

Human serum albumin interaction with paraquat studied using spectroscopic methods

The interaction between herbicide paraquat and human serum albumin (HSA) was investigated by fluorescence and UV/Vis absorption spectroscopy. Paraquat can strongly quench the intrinsic fluorescence of HSA by static quenching and nonradiative energy transferring; The hydrophobic and electrostatic interactions play a major role in stabilizing the complex. The binding site number n and apparent binding constant K_A, corresponding thermodynamic parameters ΔG, ΔH, ΔS at different temperatures, were calculated. The distance r between donor (HSA) and acceptor (paraquat) was obtained according to fluorescence resonance energy transfer. The effect of paraquat on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy.     

福建果树根部的芒果半轮线虫记述

 芒果半轮线虫(Hemicriconemoides mangiferae Siddiqi,1961)在福建省一些果园发生。寄主有龙眼(Euphoria longana)、荔枝(Litchi chinensis)、芒果(Mangifera indica)、橄榄(Canarium album)、枇杷(Eriobotrya japonica)和葡萄(Vitis vinifera)等果树。雌虫唇部正面、环纹和阴门,雄虫侧带以及幼虫角质膜刺突等细微结构用扫描电子显微镜观察。     

Investigation of the In Vivo,In Vitro,and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

Previous studies found that both oral and topical administration of enzymatic digestion products < 3 K Da ultrafiltration fractions of Pinctada martensii mantle (PMPs) had pro-healing effects. Thus, we further purified them by Sephadex-G25 and screened them by cellular assays to obtain Pinctada martensii purified peptides (PMPPs). In this study, we explored the mechanism of PMPPs on wound healing by in vivo, in vitro, and in silico experiments. LC-MS/MS results showed that PMPPs consisted of 33 peptides with molecular weights ranging from 758.43 to 2014.04 Da, and the characteristic peptide was Leu-Asp. The results of cellular assays showed that PMPPs promoted the proliferation of human skin fibroblasts (HSF) (135%) and human immortalized keratinocyte (HaCaT) cells (125%) very significantly at 12.5 μg/mL. The in vivo results showed that PMPPs could achieve scarless healing by inhibiting the inflammatory response, accelerating the epithelialization process, and regulating collagen I/III ratio. The optimal peptide sequence FAFQAEIAQLMS of PMPPs was screened for key protein receptors in wound healing (EGFR1, FGFR1, and MMP-1) with the help of molecular docking technique, which also showed to be the key pro-healing active peptide sequence. Therefore, it may provide a therapeutic strategy with great potential for wound healing.     

辣椒中可用于辅助选择的分子标记及其研究进展

分子标记辅助选择已成为植物育种领域的热点,在辣椒的育种中具有广阔的应用潜力。对分子标记辅助选择方法及其在辣椒育种中的应用进行综述,并对其发展前景进行展望。     

紫甘薯多酚氧化酶性质探讨

甘薯是世界上一种重要的粮食和能源作物,由于加工过程中其组织中的多酚氧化酶易催化多酚类物质氧化成黑色素,严重影响了其加工品质.以济薯18为材料,研究了pH值、温度、底物浓度等对其多酚氧化酶(PPO)活性的影响.结果表明:紫甘薯济薯18中PPO活性在360 am处最大,其最适反应时间为11 min,最适pH值6.5,最适温...     

利用CAPS标记辅助辣椒PVY抗性基因PVr4转育的研究

利用国外报道的与PVr4基因紧密连锁的CAPS标记,对辣椒抗PVY基因PVr4的转育进行了研究和探讨。采用与报道的相同抗源材料CdM334为回交的供体亲本,与5个自交系77013、75-7-3-1、83077、83078、83-163进行回交,在BC1代进行了分子标记的选择。从5个BC1群体75-7-3-1×(CdM334×75-7-3-1)、77013×(CdM334×77013)、83077×(CdM334×83077)、83078×(CdM334×83078)、83-163×(CdM334×83-163)中,分别检测出含有抗病连锁标记的单株21株、15株、10株、11株、12株。     

百合品种IRAP指纹图谱构建

IRAP（inter—retrotransposon amplified polymorphism）是一种基于反转录转座子的分子标记技术。文章参考烟草中的反转录转座子Tnt1的LTR保守区域设计引物,对52个百合品种进行IRAP扩增,共扩增出165条多态性带,每个品种的扩增位点数介于0～8之间,据构建的指纹图谱可将供试百合品种完全区分开。     

野生稻与栽培稻的杂种后代对褐稻虱的抗性机制初探

为了开拓抗虫基因源,培育抗性稳定的水稻抗虫品种,采用感虫的栽培稻雄性不育株为母本,以高抗褐稻虱的野生稻为父本进行有性杂交。通过用改良的苗期群体筛选法,对其后代进行抗性鉴定,证实其抗性可以遗传,已成功地获得了抗褐稻虱的杂种后代。并根据飞虱在抗虫植株上的生存率、群体建立、蜜露量和稻株的受害级别等指标与感虫对照种TN1上的差异显著性作比较,综合评定杂种后代对褐稻虱的抗性机制。结果表明,测试的N482(A)/101392组合的F_5、F_6、F_7和H_4等几个后代对褐稻虱的抗性机制均为抗生性。