海南岛几种优势树种构筑型的研究

对海南 9种优势树种构筑型进行研究 ,通过对树木分枝方式和发芽方式的细致观察和描述 ,发现有 1种属Massart型 ,1种属Troll型 ,7种属Rauh型。对这 9种树木的构筑型特征与国外的研究进行了比较 ,确定了构筑模式与属及发芽模式与属之间的关系。     

《农业与工业化》：农机化理论宝库——“发展经济学”创始人张培刚博士论文初识

发展经济学创始人张培刚1945年的博士论文《农业与工业化》,是近代农业机械化理论之集大成者。由于社会和历史的原因,这一农业机械化理论宝库一直未被认真开发、应用于我国农业机械化的实践,以致走了不少弯路。随着一代经济学大师的离世,如今,挖掘、开发这一理论宝库,用之培养新型农业机械化人才,指导我国农机化实践,必将结出丰硕之果。     

pH值对星突江鲽消化酶活性的影响

实验通过改变酶反应中的pH值,分析了星突江鲽蛋白酶、淀粉酶和脂肪酶的活性。结果表明,胃、肠和肝胰腺蛋白酶活性的最适pH值分别为3.8、8.2和7.8,各消化部位的最高点蛋白酶活性从高到低为:胃>肝胰腺>肠。胃、肠和肝胰腺淀粉酶活性的最适pH值分别为6.6、7.4和7.0,各消化部位的最高点淀粉酶活性从高到低为:肠>肝胰腺>胃。胃、肠和肝胰腺脂肪酶活性的最适pH值分别为7.4、7.8和7.8。各消化部位的最高点脂肪酶活性从高到低为:肝胰腺>肠>胃。     

锥—46抑制[^3H]次黄嘌呤掺入伊氏锥虫核酸的研究

用液体闪烁计数法对锥—46的抗锥虫作用的机理做了初步研究。发现T-46与Berenil很相似,均可显著抑制[~3H]次黄嘌呤掺入伊氏锥虫,抑制作用与浓度及时间呈正相关。T-46和Berenil对DNA合成的IC_(60)分别为1.33和1.73μg·ml~(-1)。本实验还提示T-46抗锥虫作用可能与损伤DNA模板有关。     

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress

The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10^–3, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6 M) or Lyc (0, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6, 1 × 10^–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10^–3 M VC or 1 × 10^–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10^–4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10^–3 M VC or 1 × 10^–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure.     

猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。     

长江源区土地沙漠化防治对策与措施

长江源区的土地沙漠化是最严重的生态环境问题。沙漠化土地主要分布于曲麻莱和治多两县境内的通天河阶地及楚玛尔河滩地 ,在玉树、称多县和塘古拉山乡也有分布。在青藏公路的五道粱至二道沟以东的河滩地区 ,有大片流动沙丘分布。在沱沱河口以东有连片的流动沙丘分布 ,其余地区为不连片的零星流动沙丘和沙漠化土地分布 ,长江源区共有沙漠化土地面积 1 9475 .32k 。土地沙漠化的迅速扩展 ,不仅影响本区生态环境 ,同时直接影响着整个流域的社会经济持续发展。根据本区高海拔寒冷的严酷自然条件 ,依科技为先导 ,建立科技示范工程 ,以保护生态环境为主 ,恢复植被与防治沙漠化土地相结合 ,即采取封育保护或建立生态保护区 ,在条件适宜的地区结合补播、治沙造林 ,运用生物和工程相结合的综合防治土地沙漠化的技术措施 ,建立新的生态系统 ,改善生态环境 ,恢复和重建长江源区的生态环境     

Technical note: recombinant LHRH fusion protein suppresses estrus in heifers

A recombinant luteinizing hormone-releasing hormone (LHRH) fusion protein was evaluated for its effectiveness in suppression of estrus in heifers. Eight heifers were randomly assigned to two equal treatment groups. Treatments consisted of recombinant ovalbumin-LHRH-7 or recombinant ovalbumin (control). This recombinant chimeric fusion protein consisted of ovalbumin with seven LHRH peptides (ovalbumin-LHRH-7). The plasmid for this protein was expressed in E. coli and was collected and purified as an insoluble protein. One milligram of the respective proteins was suspended in 2 mL of Z-Max adjuvant and administered by intramammary injection three times at 7-wk intervals. Luteinizing hormone-releasing hormone antibody binding was elevated in heifers treated with ovalbumin-LHRH-7 compared to ovalbumin-treated heifers (P < .05). Serum progesterone concentrations (< 1 ng/mL) indicate that the estrous cycle of the four heifers treated with ovalbumin-LHRH-7 was suppressed for a time period ranging from 60 to 238 d, which was different from control heifers (P < .01). Serum progesterone for the control heifers continued to exhibit cyclic profiles over the experimental period. This preliminary study in heifers demonstrated that a chimeric LHRH fusion protein induced elevated concentrations of circulating LHRH antibodies that suppressed estrus for an average of 122 +/- 41 d.