全文获取类型
收费全文 | 7454篇 |
免费 | 488篇 |
国内免费 | 561篇 |
专业分类
林业 | 648篇 |
农学 | 351篇 |
基础科学 | 247篇 |
571篇 | |
综合类 | 3606篇 |
农作物 | 542篇 |
水产渔业 | 334篇 |
畜牧兽医 | 1311篇 |
园艺 | 638篇 |
植物保护 | 255篇 |
出版年
2024年 | 56篇 |
2023年 | 141篇 |
2022年 | 334篇 |
2021年 | 293篇 |
2020年 | 314篇 |
2019年 | 287篇 |
2018年 | 219篇 |
2017年 | 365篇 |
2016年 | 236篇 |
2015年 | 373篇 |
2014年 | 415篇 |
2013年 | 450篇 |
2012年 | 625篇 |
2011年 | 654篇 |
2010年 | 615篇 |
2009年 | 560篇 |
2008年 | 524篇 |
2007年 | 498篇 |
2006年 | 398篇 |
2005年 | 354篇 |
2004年 | 197篇 |
2003年 | 119篇 |
2002年 | 132篇 |
2001年 | 147篇 |
2000年 | 121篇 |
1999年 | 36篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 3篇 |
1990年 | 6篇 |
1989年 | 1篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1956年 | 5篇 |
排序方式: 共有8503条查询结果,搜索用时 15 毫秒
211.
212.
213.
214.
茎芥菜胞质四倍体白菜雄性不育系花药发育的研究 总被引:1,自引:0,他引:1
以茎芥菜胞质雄性不育系与四倍体白菜杂交获得的同源四倍体白菜异源胞质雄性不育系及其保持系为材料,采用形态学和石蜡切片方法研究其花药解剖结构及发育。结果表明:该雄性不育为结构性雄性不育,其退化或畸形雄蕊分为5种类型:盾状雄蕊、条状雄蕊、片状雄蕊、羽状雄蕊和瓣状雄蕊。该雄性不育系花药发育败育有两个时期,盾状雄蕊花药败育于孢原细胞分化期,雄蕊整个发育时期均处在孢原细胞分化期,无绒毡层与花粉母细胞的分化,不形成药室,属孢子体败育型;其它类型雄蕊,花药败育发生在雄蕊原基分化时期,由于雄蕊原基偏离正常的分化轨道,形成瓣状化雄蕊。 相似文献
215.
AIM: The different effect of bivalent immunoglobulin Yolk (IgY) was evaluated against snake venom between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation. METHODS: The venom of naja and viper was injected alternately into the leghorn hen. Bivalent anti-snake venom IgY was extracted by water dilution. The concentration of bivalent IgY in plasma was observed in indirect ELISA assay after bivalent anti-snake venom IgY taken orally. The gastric emptying function test was used for determining optimization time after gastric administration of IgY. The protective effect of bivalent anti-snake venom IgY was compared between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation. RESULTS: Bivalent anti-snake venom IgY was extracted from eggs laid in 28-42 d after the first immunization. The titers of Bivalent IgY against cobra and viper venom were 1∶12 800 and 1∶6 400. At the time of 2.5-3.5 h after bivalent anti-snake venom IgY was taken orally in three concentrations (75 mg, 150 mg, 300 mg·0.5 mL-1·20 g-1 BW), the gastric evacuation rate of mice was above 68.9%, with the plasma concentration of bivalent IgY in peak. The survival time of mice envenomation with snake venom was extremely prolonged (P<0.01), after IgY was taken by intragastric administration or intraperitoneal injection. While administration with the same dose of IgY, the survival rate of mice envenomation with cobra venom was higher than that of viper venom. The effective dose of intragastric administration was decuple higher than that of intraperitoneal injection. CONCLUSION: The animal envenomation with cobra or viper venoms can be significantly protected by bivalent anti-snake venom IgY with intragastric administration or intraperitoneal injection. 相似文献
216.
WU Xiao-bin PENG Jun-sheng LI Chu-jun WANG Hui DU Yan-ping YANG Zu-li XIANG Jun HU Kun-hua LIU Wei LI Ming-tao 《园艺学报》2009,25(6):1098-1103
AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma. 相似文献
217.
低温胁迫对四种野生常绿藤本植物抗寒生理指标的影响 总被引:4,自引:1,他引:3
以鹰爪枫Holboellia coriacea、南五味子Kadsura japonica、尾叶挪藤Stauntonia obovatifoliola ssp. urophylla、山蒟Piper hancei等4种野生常绿藤本植物为试验材料,通过人工冷冻处理,对叶片的相对电导率、过氧化物酶(POD)活性和游离脯氨酸含量等3个生理指标进行了测定.结果表明:细胞膜透性表现为逐渐上升趋势;而过氧化物酶(POD)活性和游离脯氨酸含量则随着温度的降低呈现出先上升后下降的变化规律,低温下耐寒性强的种类能保持较高的POD活性.4种野生常绿藤本植物的抗寒性具有明显差异,其中以鹰爪枫抗寒能力最强,其次为南五味子和尾叶挪藤,山蒟抗寒能力最差. 相似文献
218.
生物质成型燃料加工技术分析研究 总被引:2,自引:0,他引:2
阐述了生物质成型燃料国内外发展现状、生物质成型燃料加工技术与装备的特点与使用对象;重点分析了现有的生物质成型燃料加工技术和装备在实际应用中的优缺点,得出目前我国的生物质固化成型装备在设备的实用性、系列化、规模化上还很不足,距国际先进水平还有不小的差距;这一问题以成型机最为突出,表现在生产率低、成型能耗高、主要工作部件寿命短、机器故障率多、费用高等方面;并依据生物质成型燃料发展趋势,提出目前我国生物质成型燃料加工设备的设计要点为:一是加工设备的原料适应性,二是加工设备的先进性与性价比。 相似文献
219.
拦河闸下游河床下切、河道水位下降,会造成其下游消能工无法正常运行,需采取相应的工程措施解决。本文根据广东省流溪河大坳拦河闸、东江水利枢纽左河汊拦河闸、乌石拦河闸、潮州供水枢纽西溪拦河闸等四座拦河闸下游消能工除险加固的水力模型试验成果,介绍了拦河闸下游河道水位下降后的下游消能工改造优化方案及运行情况,供类似工程设计和运行参考。 相似文献
220.
综合运用基于DEM的流域水系模型与TOPMODEL水文模型构建出了流域梯级水电站实时水文预报模型,并以VS.Net为平台,运用分布式网络编程技术将二者集成,为西南某流域梯级水电站设计开发出功能先进和完善的网络版实时水情预报管理软件系统.该系统已投入使用,为实现流域水情预报的智能化、信息化和网络化管理提供了可靠的辅助工具. 相似文献