不同灌溉制度对温室番茄光合特性的影响

光合对植物生长有直接影响,是作物产量形成的基础,因此研究不同灌溉制度对温室番茄生理变化的影响,对于温室番茄生产有积极的意义.试验采用传统灌溉方式为对比,研究了温室番茄滴灌条件下的灌溉制度对其光合参数及水分利用效率的影响.结果表明:滴灌可以使温室番茄的净光合速率峰值提前,提高水分利用效率;番茄的气孔导度与净光合速率、蒸腾速率、水分利用效率显著相关;灌水量较低时,可以通过缩短灌水间隔来提高温室番茄的水分利用效率.     

皇甫川流域水沙变化特点及其趋势分析

以地处多沙粗沙区的皇甫川流域为例,采用实测资料,对其水沙量进行了统计分析.采用滑动平均法,Mann-Kendall非参数秩次相关检验法.对控制站皇甫站水沙变化趋势进行了研究.结果表明:在一定显著性水平下,皇甫川流域径流与泥沙在多年变化中均呈明显下降趋势.最后,针对影响水沙变化的降水与人类活动因素,应用不同时段的雨-水-沙关系,结合双累积曲线的变化过程,定量计算了不同时段的水沙衰减量以及由于雨水衰减影响和水土保持影响的减沙量所占比重.     

割茎高度和割茎时间对潞党参产量和含量的影响

采用不同割茎高度和割茎时间处理,观察各处理对潞党参产量和含量的影响。结果表明：9月25日割茎保留地上部分20cm后,经过冬季养分积累,春季产量较秋季有所提高,4月30日达到最大产量,适宜春季采挖党参。从割茎高度来看,割茎保留60~80 cm地上部分时,产量达到最大,小区产量为164.75g,鲜根折合产量为274.5 kg·667 m^-2。从割茎时间上看,在8月13日割茎小区产量达最大为164.75g。11月10日采挖党参时,浸出物含量达最大值为68.06%,较对照提升1.75%。     

新农菜豆1号密度试验初报

研究了新农菜豆1号在3种不同密度下,农艺性状、干物质积累、叶面积指数等的变化规律。研究结果表明:随着密度的增加,株高和底荚高度增加,茎粗、节数、分枝数、分枝总长降低,单株荚数、单株粒数、单株粒重降低,而百粒重与密度关系不显著。以密度21万株·hm-2鲜荚产量最高,为12440kg·hm-2,百粒鲜重64.7g。     

Analysis of QTL for Seed Dormancy and Their Response to Dry Heat Treatment in Rice(Oryza sativa L.)

Quantitative trait loci(QTL)controlling seed dormancy in rice were identified using recombinant inbred lines(RILs)population derived from the cross between a japonica variety Kinmaze and an indica variety DV85. Seeds of two parental cultivars and each RIL were harvested in 35d after heading.. The germination percentage of these seeds at 30℃for 7 days were measured as the degree of seed dormancy.. QTL analysis was performed with Windows QTL Cartographer 1.13a program by composite interval mapping.. A total of four QTL for seed dormancy were detected on chromosome 2(two regions),5 and 11,respectively.Phenotypic variation explained by each QTL ranged from 8.37 to 17.40%.. Responses of such loci to a dormancy-breaking treatment with dry heat were further detected. The results showed that two alleles of qDOR-2-1 and qDOR-5 from DV85 as well as the allele of qDOR11 from Kinmaze increased the seed dormancy,which seemed to be easily broken by dry heat treatment. Such loci of seed dormancy may be applied to rice genetic improvement.The allele of qDOR-2-2 from DV85 increased the seed dormancy,which could not be broken by dry heat treatment.     

转基因抗虫作物中豇豆胰蛋白酶抑制剂（CpTI）酶联免疫检测方法的建立

 从10个不同豇豆品种中筛选出抑制剂活性最高的901青皮豇豆，提取豇豆胰蛋白酶抑制剂。通过G-75凝胶过滤、亲和层析纯化了豇豆胰蛋白酶抑制剂（CpTI）并制备了其兔多克隆抗体。用所制备的抗体建立了酶联免疫检测方法并检测了转CpTI编码基因棉花、水稻中的CpTI含量，发现棉花不同器官CpTI的表达量不尽相同。     

Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato

Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang wasconducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase (GUS) and neomycin phosphotransferase (NPT Ⅱ ) genes. The results indicated that embryogenicsuspension cultures precultured for 1 -3 d were suitable for the transformation. The optimal cocultivation timewas 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.     

甘薯胚性悬浮细胞遗传转化的研究

 以甘薯品种栗子香的胚性悬浮细胞为材料 ,以A2 0 8SE(pROA93)为供体菌株 ,对甘薯胚性悬浮细胞遗传转化的基本条件进行了研究。结果表明 ,对胚性悬浮细胞预培养 1～ 3d有利于转化 ,适宜的共培养时间为 4～ 5d ,适宜的Kan浓度为 5 0～ 75mg·L-1(悬浮培养阶段 )和 10 0mg·L-1(增殖培养阶段和植株再生阶段 ) ,适宜的Carb浓度为 10 0mg·L-1。获得了经GUS检测及PCR检测的转基因植株。     

丸粒化玉米种子的薄层干燥试验研究

利用丸粒化玉米种子干燥试验装置，研究了风温、风速、加热时间对丸粒化玉米种子干燥速率的影响。同时，采用正交试验方法，对丸粒化玉米种子进行了三因素三水平干燥试验，并建立了干燥特性曲线，分析了各试验因素对干燥特性的影响。结果表明：风温越高，风速越大，加热时间越长，干燥速度越快。