社会主义核心价值观践行中党员干部责任倦怠及解决路径

随着党的群众路线教育实践活动的深入开展,社会主义核心价值观践行中党员干部的责任倦怠问题较为突出,主要表现在工作态度、工作效率、工作目的等多方面。培育党员干部责任意识应从重言行、知礼仪、讲品德、树信仰等方面下功夫,以艰苦奋斗、求真务实的精神克服责任倦怠,树立党员干部新形象。     

无花果新品种‘紫宝’

‘紫宝’是从中国无花果主栽品种‘青皮’中获得的芽变品种。秋果平均横径33.6 mm,纵径35.7 mm,平均单果质量33.4 g;果皮紫色,果肉深红色,可溶性固形物18.5°Brix,果肉松软,果汁量适中,味道香甜,品质优。     

褚橙龙陵基地柑橘叶片DRIS图解法和指数法综合营养诊断分析

[目的]开展基于综合营养诊断施肥法(Diagnosis and Recommendation Integrate System,DRIS)的冰糖橙种植基地柑橘营养诊断,为柑橘园的营养诊断和平衡施肥提供参考依据.[方法]以云南褚橙龙陵基地500 ha冰糖橙为研究对象(柑橘园随机划分为8个区域),通过测定成熟秋梢叶片中氮(N)、磷(P)、钾(K)、钙(Ca)、镁(Mg)、铜(Cu)、锌(Zn)和铁(Fe)矿质营养的含量,运用DRIS图解法求得冰糖橙叶片各营养元素诊断参数最适比范围,依据DRIS指数法求得各区域冰糖橙需肥紧迫程度排序及养分不平衡指数(NII),初步制定冰糖橙树体营养元素DRIS指数分级标准.[结果]整个园区高产园(>22500 kg/ha)的各营养元素含量大多高于低产园(≤22500 kg/ha),高产园和低产园间N、Fe、N/Fe、P/Fe、Ca/Mg、Ca/Fe、Mg/Zn差异显著(P<0.05)或极显著(P<0.01).当N/P在2.27~2.57,N/K在1.58~1.91,P/K在0.66~0.79时冰糖橙叶片N、P和K养分含量平衡;当Ca/Mg、Ca/Fe、Mg/Fe、Cu/Zn、Cu/Fe和Zn/Fe分别在17.01~19.09、0.21~0.26、0.012~0.015、2.77~4.60、0.30~0.43和0.09~0.12时,冰糖橙叶片Ca、Mg、Fe、Zn和Cu养分处于平衡状态.综合诊断显示冰糖橙叶片养分需求顺序为Mg>N>K>P>Ca>Cu>Zn>Fe,不同区域冰糖橙养分需求顺序不同,高产园冰糖橙相对缺乏的养分有N、P,低产园相对缺乏的养分有N、Mg,相比之下,低产园营养比例失衡情况较严重.依据高产园叶片矿质营养元素含量范围确定冰糖橙叶片矿质营养元素的适宜值为N(11.66~13.30 g/kg)、P(4.74~5.61 g/kg)、K(6.23~8.23 g/kg)、Ca(13.67~19.33 g/kg)、Mg(0.86~0.97 g/kg)、Cu(14.49~37.71 mg/kg)、Zn(6.38~8.02 mg/kg)和Fe(55.91~85.74 mg/kg).[结论]根据DRIS诊断,褚橙龙陵基地冰糖橙园普遍存在N、P、K、Ca、Mg缺乏,Cu、Zn、Fe相对过剩问题.DRIS图解法和指标法综合诊断既可求得各元素最适比范围和需肥紧迫程度,同时,参照冰糖橙叶片养分含量分级标准能判断树体营养元素的平衡状况,可用于生产上指导下一年合理施肥.     

OsHemA gene,encoding glutamyl-tRNA reductase(GluTR) is essential for chlorophyll biosynthesis in rice(Oryza sativa)

Chlorophyll(Chl) biosynthesis is essential for photosynthesis and plant growth. Glutamyl-tRNA reductase(GluTR) catalyzes glutamyl-tRNA into glutamate-1-semialdehyde(GSA) and initiates the chlorophyll biosynthesis. Even though the main role of GluTR has been established, the effects caused by natural variations in its corresponding gene remain largely unknown. Here, we characterized a spontaneous mutant in paddy field with Chl biosynthesis deficiency, designated as cbd1. With intact thylakoid lamellar structure, the cbd1 plant showed light green leaves and reduced Chl and carotenoids(Cars) content significantly compared to the wild type. By map-based gene cloning, the mutation was restricted within a 57-kb region on chromosome 10, in which an mPingA miniature inverted-repeat transposable element(MITE) inserted in the promoter region of OsHemA gene. Both leaf color and the pigment contents in cbd1 were recovered in a complementation test, confirming OsHemA was responsible for the mutant phenotype. OsHemA was uniquely predicted to encode GluTR and its expression level was dramatically repressed in cbd1. Transient transformation in protoplasts demonstrated that GluTR localized in chloroplasts and a signal peptide exists in its N-terminus. A majority of Chl biosynthesis genes, except for POR and CHLG, were down-regulated synchronously by the repression of OsHemA, suggesting that an attenuation occurred in the Chl biosynthesis pathway. Interestingly, we found major agronomic traits involved in rice yield were statistically unaffected, except for the number of full grains per panicle was increased in cbd1. Collectively, OsHemA plays an essential role in Chl biosynthesis in rice and its weak allele can adjust leaf color and Chls content without compromise to rice yield.     

Effects of Adding Mannan Oligosaccharides to Diets with Different Concentrate to Forage Ratios on Ruminal Fermentation of Sheep in vitro

The test was aimed at determining the effects of adding MOS to diets with different concentrate to forage ratios on ruminal fermentation of sheep in vitro.The 4×6 two-factor experimental design was chosen.There were 4 diets with different concentrate to forage ratios(20:80, 30:70, 40:60 and 50:50) and added to 6 dosages MOS(0, 0.4%, 0.8%, 1.2%, 1.6% and 2.0%), respectively.The method of gas production in vitro was used to measure the total gas and CH₄ production, IVDMD, IVCPD and IVOMD after cultured for 3, 6, 9, 12 and 24 h.The results showed that the gas(except 12 and 24 h) and CH₄ production(except 9 h) were not impacted by the two factors(P>0.05).The IVDMD, IVCPD and IVOMD were significantly affected by concentrate to forage ratios(P<0.05), while the IVCPD at 24 h was significantly affected by MOS(P<0.05).Meanwhile, all the indexes were not influenced by the interaction of two factors(P>0.05).The IVDMD and IVOMD increased with the rising of concentrate approximately, while the IVCPD decreased with the rising of MOS roughly.The biggest associative effect was gained in 30:70 concentrate to forage diet when adding 1.2% MOS.     

烤烟配方营养土定制化生产机械研制

在充分调查论证和反复试验基础上，确定以饼肥、菌渣、牛粪和钙镁磷肥的配方营养土替代传统烤烟火土灰配方营养土。根据饲料粉碎搅拌加工一体化原理，研制烤烟营养土加工机械。针对混制箱的堵塞问题，通过改进混制系统的粉碎和搅拌方式予以消除。试点机械化、规模化制作烤烟营养土，商品化供应烟农，简化烟农营养土制作工序，降低烟农营养土制作成本，满足烟叶生产规模化降本增效和烟叶生产绿色发展的需要，具有较好的社会和经济效益。      

“人的城镇化”与图书馆全方位知识服务

人的城镇化是新型城镇化的核心。在此分析了"人的城镇化"的内涵、中国在"人的城镇化"中面临的问题,并结合作为全国统筹城乡综合配套改革试验区的重庆市的现状,探寻"人的城镇化"问题的根源。基于重庆市南岸区参与新型城镇化工作试点的实践基础,提出在"人的城镇化"进程中,图书馆全方位知识服务的应对策略。     

UV/H2O2降解水体中磷酸三（1,3-二氯-2-丙基）酯的研究

以磷酸三(1,3-二氯-2-丙基)酯(TDCP)作为研究对象,探索了TDCP的紫外光氧化高效降解方法。结果表明,紫外光光强对TDCP的降解有很大的影响。当TDCP初始浓度为10 mg/kg,紫外光光强为94.5×100μW/cm2,反应时间为60 min,加入H2O2浓度为5.00 mmol/L,溶液起始pH为7时,TDCP的TOC去除率为93.69%,有机氯、有机磷完全转化为Cl-和PO43-;UV/H2O2体系能有效的降解TDCP。     

鸭坦布苏病毒E蛋白具有独特交叉反应性中和表位的鉴定

利用抗鸭坦布苏病毒(DTMUV)中和单抗1G2,结合E蛋白多肽扫描技术,鉴定了最小抗原表位²²⁷GSSAGTWQN²³⁵。Western blot显示,残基²³¹G或²³³W的突变后,表位完全失去了与1G2抗体识别的功能,表明²³¹G或²³³W是抗体1G2识别的关键氨基酸位点。通过免疫荧光分析(IFA)显示,MAb 1G2与JEV、WNV和ZIKV的E蛋白发生交叉反应,表明该表位是黄病毒的交叉反应性表位。蛋白质和病毒模型显示,表位定位于成熟病毒粒子可接近的表面,在E蛋白结构域Ⅱ的hi环中。本研究首次证明,中和抗体1G2靶向交叉反应表位定位在E蛋白结构域Ⅱ的hi环,该表位的鉴定有助于提高对黄病毒血清诊断中存在交叉反应问题的认识和理解。     

中国饮料制造业全要素生产率测度及其影响因素分析——基于13个四位数行业的视角

基于2003-2010年间中国饮料制造业13个四位数行业的面板数据,运用非参数数据包络分析的DEA-Malmquist生产率指数方法,对饮料制造业整体及细分行业的全要素生产率(TFP)进行测度,并考察TFP指标的变动情况。在此基础上,运用Tobit模型对行业TFP的影响因素进行了探讨。结果表明,考察期间,技术进步是推动TFP增长的主要动力;人均资本、企业规模和劳动力成本与饮料制造业TFP间具有显著的正相关性,而出口交货值和市场竞争程度两项指标与TFP间不显著负相关。