水稻条纹病毒在玉米和水稻上流行差异分析

为了明确水稻条纹病毒(RSV)在水稻和玉米上发生程度差异明显的原因,从一个侧面了解RSV流行本质,2004年在洪泽进行田间试验。采用黄盘诱集、盘扑、盘刮、肉眼计数等方法比较武育粳3号和掖单13上灰飞虱侵入和消长动态,结果表明两者均只有一个成虫侵入高峰,单位面积迁入虫量前者是后者的3.6倍,单株平均虫量相近。Dot-ELISA法测定灰飞虱带毒率为40%,成虫迁移扩散高峰期22d内两者接毒量约为每天百株137头带毒虫。武育粳3号有二代若虫发生,掖单13则无。逐日调查发病进程,结果显示水稻发病率为60%,玉米为0。室内抗性鉴定,掖单13对RSV的抗性比武育粳3号高4个级别。综合以上结果,寄主品种抗性决定了RSV在水稻和玉米上流行状况,二代若虫重复侵染是RSV在水稻上重发的另一主要原因。     

Detection of a broad range of potato viruses in a single assay by mechanical inoculation of herbaceous test plants

To optimize mechanical inoculation of test plants as part of the regulated post-entry quarantine testing of stolon- and tuber-forming Solanum spp. (including potato), nine test plant species have been screened for their ability to detect 22 potato-infecting viruses. These included all the common mechanically transmissible potato viruses and most of the viruses found in potato incidentally. The symptoms observed after mechanical inoculation are shown for each combination of test plant species and virus. Under given conditions, Nicotiana occidentalis -P1 and Nicotiana hesperis -67A were found to detect reliably 20 and 18 out of 22 viruses respectively. These and former results on the seed-transmissible viruses and Andean potato mottle virus demonstrate that these Nicotiana species are very suitable for post-entry quarantine testing. Addition of either Chenopodium amaranticolor or Chenopodium quinoa to these two species may slightly extend the range of viruses. Therefore, using C. amaranticolor / C. quinoa , N. hesperis -67A and N. occidentalis -P1 for the biological screening of imported Solanum spp. will improve both the efficiency and the quality of post-entry quarantine testing.