苯丙烯酸对黄瓜幼苗光合作用和细胞超微结构的影响

 通过基质栽培研究不同浓度的苯丙烯酸对黄瓜幼苗光合作用和超微结构的影响。结果表明，苯丙烯酸浓度在80 µmol·L 时显著地降低了黄瓜幼苗的叶面积、叶绿素a含量、光合速率、蒸腾速率、根系活力和气孔张开数(P<0.05)；叶绿体和线粒体等超微结构也受到破坏，而浓度在150 µmol·L 时显著减少了全株干质量和气孔总数，细胞器出现解体现象。     

百合花发育相关MADS box基因的克隆

 利用RT-PCR的方法，从百合中克隆得到了3个接近全长的花发育相关MADS box基因片段 LfMADS1-3。其中LfMADS1和LfMADS3以往未见报道，分别与多种作物的AG、SEP同源基因具有较高的同源性；LfMADS2与已发表的LMADS2对应区段的氨基酸同源性高达98.99%，推测可能是同一个基因。     

铺散诸葛菜的组织培养及植株再生

 以铺散诸葛菜的叶柄及叶片为外植体, 研究了不同的再生方式。在6-BA 和NAA 配合使用的MS 培养基上, 叶柄和叶片外植体有高的成芽率, 具有强的器官再生能力。在2,4-D 与6-BA、NAA 结合的培养基上, 叶柄和叶片有较高的愈伤组织诱导率, 在含6-BA 的培养基上也易于分化。叶柄、叶片再生的芽和经愈伤组织分化而得来的芽快速繁殖后, 在生根培养基上形成完整植株。研究表明铺散诸葛菜具有极强的器官分化能力。     

应用真空熏蒸技术杀灭蔗扁蛾

利用真空熏蒸技术对受蔗扁蛾Opogona sacchari Bojer危害的发财树进行了真空熏蒸处理.结果表明,在真空条件下,仅需要较低的熏蒸剂量在较短的时间内即可全部杀死发财树里的蔗扁蛾,杀虫效果达100%,达到快速、高效、安全、环保的要求.为花卉害虫的处理提供了一种新的方法.     

不同光湿环境对葡萄花芽分化和叶绿体类囊体膜磷酸酯酶活性的影响

以欧亚种葡萄美人指(Vitisviniferacv.ManicureFinger)为试材,正常光湿为对照,采用正常光偏高湿、偏弱光正常湿度、偏弱光高湿、偏弱光临界高湿、临界弱光偏高湿和临界弱光临界高湿6种处理。试验表明:单一偏弱光处理,葡萄叶片叶绿素含量上升,其余处理叶绿素含量下降;6种处理叶绿体类囊体膜磷酸酯酶活性都低于对照,叶绿体类囊体膜磷酸酯酶活性与光照和湿度有关;正常光偏高湿和偏弱光正常湿度部分芽能分化花原基,但在偏弱光高湿或偏弱光临界高湿花芽分化质量严重下降,临界弱光偏高湿和临界弱光临界高湿几乎未发现花原基;单一的弱光因子比单一的高湿因子对叶绿体类囊体膜磷酸酯酶活性和花芽形态分化影响更大,但弱光与高湿同时存在比单一弱光或高湿因子作用于葡萄时,叶绿体类囊体膜磷酸酯酶活性下降更为显著,花芽更难以形成。     

不同副梢处理对赤霞珠葡萄生长和结果的影响

试验以3～5年生赤霞珠葡萄(CabernetSauvignon)为试材,对其进行了不同的副梢处理:只留顶端1个副梢、副梢绝后处理、花序以上反复摘心、副梢全留。2年的结果表明:副梢全留(所有副梢全留2～3片叶反复摘心)处理的叶面积指数高,果实的产量和品质高。因此,相对多留副梢叶片,改善了叶幕叶龄结构,提高了果实品质,有利于枝梢成熟。     

利用雄性不育两用系配制的辣椒一代杂种‘冀研8号’

 ‘冀研8号’是利用甜椒雄性不育两用系AB91-8为母本，以优良辣椒自交系L16-2为父本配制成的杂交一代辣椒新品种。该品种早熟，果实牛角形，商品性好，单果质量70 g，味辣，抗病毒病，较抗疫病，适宜华北地区春季塑料大拱棚和露地地膜覆盖栽培，产量49．6～63．6 t／hm 。     

山杏新品种‘甜仁山杏’

 ‘甜仁山杏’是从山杏(苦杏仁) 中选出的甜仁新品种。其种子不具苦味, 果实、果核及种子比原变种小。可用于山区造林, 种子用于深加工。     

Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro

AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro.     

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.