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51.
为了回顾过去,把握未来,中文简单回顾和比较了中日两国林业发展的道路的各自长短,并为加强合作的必要性和可能性提出了一些直观的看法。 相似文献
52.
Yoshizawa K Sasaki T Uehara N Kuro M Kimura A Kinoshita Y Miki H Yuri T Tsubura A 《Journal of toxicologic pathology》2012,25(1):27-35
Seven-week-old male Lewis rats received a single intraperitoneal injection of N-ethyl-N-nitrosourea (ENU) (100, 200, 400 or 600 mg/kg), and retinal damage was evaluated 7 days after the treatment. Sequential morphological features of the retina and retinal DNA damage, as determined by a TUNEL assay and phospho-histone H2A.X (γ-H2AX), were analyzed 3, 6, 12, 24 and 72 hr, 7 days, and/or 30 days after 400 mg/kg ENU treatment. Activation of the nuclear enzyme poly (ADP-ribose) polymerase (PARP) was analyzed immunohistochemically by poly (ADP-ribose) (PAR) expression in response to DNA damage of the retina. All rats that received ≥ 400 mg/kg of ENU developed retinal degeneration characterized by the loss of photoreceptor cells in both the central and peripheral retina within 7 days. In the 400 mg/kg ENU-treated rats, TUNEL-positive signals were only located in the photoreceptor cells and peaked 24 hr after ENU treatment. The γ-H2AX signals in inner retinal cells appeared at 24 hr and peaked at 72 hr after ENU treatment, and the PAR signals selectively located in the photoreceptor cell nuclei appeared at 12 hr and peaked at 24 hr after ENU treatment. However, degeneration was restricted to photoreceptor cells, and no degenerative changes in inner retinal cells were seen at any time points. Retinal thickness and the photoreceptor cell ratio in the central and peripheral retina were significantly decreased, and the retinal damage ratio was significantly increased 7 days after ENU treatment. In conclusion, ENU induced retinal degeneration in adult rats that was characterized by photoreceptor cell apoptosis through PARP activity. 相似文献
53.
Yamada Y Sato R Kobayashi S Hankanga C Inanami O Kuwabara M Momota Y Tomizawa N Yasuda J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):443-448
Lactoferrin has several biological activities, including antitumor activities in some human and animal tumor cells. Clinical trials have been carried out in human medicine based on these effects. However, the antitumor effects of lactoferrin in veterinary medicine remain unknown. In this in vitro study, we demonstrated that co-incubation of canine mammary gland tumor cells (CIPp and CHMp) and bovine lactoferrin induced growth arrest of tumor cells. This growth arrest was associated with induction of G1 arrest. Furthermore, this effect was stronger in tumor cells than in normal cells. These findings demonstrate that bovine lactoferrin has anti-tumor activity in canine mammary tumors and has the potential for use in tumor-bearing dogs. 相似文献
54.
Yoshitaka Ono Sinchai Chatasiri Siriporn Pota Yuichi Yamaoka 《Journal of General Plant Pathology》2012,78(5):338-347
A new grapevine leaf rust (GLR) was found to be caused by Phakopsora montana. This new fungus naturally infects Crimson Glory vine (Vitis coignetiae), forming uredinia and telia on the leaves. Under experimental conditions, the fungus was pathogenic to table grape cultivars Delaware and Kyoho (V.?×?labruscana), Amur River grape (V. amurensis), and V. ficifolia, on which it produced urediniospores. Inoculation experiments proved that the pathogen alternates hosts, forming spermogonia and aecia on Meliosma tenuis. The new pathogen resembles P. meliosmae-myrianthae (=P. euvitis), a common GLR fungus; however, its spermogonial/aecial stage is restricted to M. tenuis, contrary to P. meliosmae-myrianthae with its spermogonial/aecial stage restricted to M. myriantha. Aeciospores of the new pathogen are evenly thin-walled, whereas the aeciospore wall is conspicuously thickened apically in P. meliosmae-myrianthae. Phakopsora montana is known to occur only on V. coignetiae in nature; however, table grape cultivars Delaware and Kyoho were not resistant to P. montana under experimental conditions. These results indicate that P. montana has caused a certain proportion of the GLR disease recorded in Japan with a possible mixed infection with P. meliosmae-myrianthae. 相似文献
55.
Kazutaka Kido Masaru Hasegawa Hiroyuki Matsumoto Masaki Kobayashi Yuichi Takikawa 《Journal of General Plant Pathology》2010,76(3):208-218
Ninety-six strains of Pantoea ananatis were isolated from 14 plant species including melon, rice, tea and other crops of economic importance. They were classified
into three groups (group I, II, III) based on a welsh onion stabbing assay, tobacco infiltration test, and polymerase chain
reaction to detect indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). Group Ι strains were characterized as causing significant blight symptom on welsh onion and inducing a hypersensitive response
(HR)-like reaction on tobacco leaves after 36–48 h and encompassed 20 isolates from foxtail millet, hydrangea, pineapple,
river water and rice. These 20 isolates did not possess iaaM, iaaH, or etz genes. Group II, consisting of 34 melon isolates, harbored iaaM,
iaaH and etz genes, but did not cause either blight on welsh onion or HR-like reaction on tobacco. Group III strains did not have the
iaaM, iaaH, and etz genes, nor did they cause any reaction on welsh onion or tobacco. The 42 strains in group III were isolated from bamboo grass,
Chinese silver grass, citrus, dogwood, melon, mugwort, silk tree, sweet corn, tea and welsh onion. Representative strains
of the three groups were tested for pathogenicity on melon and rice. Group Ι strains caused palea browning on rice but not
internal fruit rot on melon. On the contrary, group II strains did not cause disease on rice but caused internal fruit rot
on melon. Group III strains were not pathogenic on rice or melon. These results suggested that the host range of P. ananatis may be predicted by the reactions of welsh onion and tobacco and detection of iaaM, iaaH and etz genes. These tools may serve as rapid tests to identify the pathogenicity groups of P. ananatis. 相似文献
56.
Takahashi H Inumaru S Takahashi M Watanabe S Iga K Yokomizo Y Geshi M Okano A Okuda K 《The Journal of reproduction and development》2003,49(6):433-440
Bovine interferon (bIFN) tau, which plays a key role in maternal-fetal recognition of pregnancy, was expressed by an Autographa californica nuclear polyhedrosis virus expression system. cDNA coding bIFNtau was derived from cultured trophoblast cells. The recombinant (r) bIFNtau had high antiviral activity (1 x 10 (8) IU/mg) and the molecular weight of rbIFNtau was estimated to be 23 kDa by Western blotting analysis. We investigated the biological effect of rbIFNtau on prostaglandin (PG) F(2alpha) synthesis in cultured bovine endometrial epithelial cells in the presence or absence of oxytocin (OT, 100 nM). rbIFNtau suppressed basal and OT-induced PGF(2alpha) production in a dose-dependent manner (1-1,000 ng/ml). These results showed that biologically active rbIFNtau was produced in the baculovirus expression system, and that rbIFNtau had the ability to suppress the synthesis of PGF(2alpha) from bovine endometrial epithelial cells. 相似文献
57.
High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system 总被引:9,自引:0,他引:9
Nagata T Ishikawa S Shimokawa E Kamikawa M Kikuma R Muneta Y Yokomizo Y Nakamura M Takehara K 《Veterinary immunology and immunopathology》2002,87(1-2):65-72
Bioactive recombinant bovine interleukin-18 (rboIL-18) was expressed using a baculovirus system. Normally, IL-18 is translated as a precursor form of a 24kDa polypeptide and processed by IL-1beta converting enzyme (ICE) to a mature bioactive form of 18kDa protein. Hence, to express active form IL-18, we constructed two recombinant baculoviruses containing boIL-18 and human ICE (hICE) genes, respectively, and superinfected these viruses into insect cells. Superinfection of both recombinant viruses into the cells resulted in the expression of a 24kDa precursor form and an 18kDa mature form detectable in the supernatant by immunoblotting using anti-porcine IL-18 antibody. Culture supernatant from the superinfected cells showed a synergistic effect with recombinant boIL-12 for production of interferon-gamma (IFN-gamma) in bovine peripheral mononuclear cells. By addition of histidine hexamer at the C-terminal of boIL-18, the mature IL-18 was purified. Bioactivity remained after purification. 相似文献
58.
Takehara K Kikuma R Ishikawa S Kamikawa M Nagata T Yokomizo Y Nakamura M 《Veterinary immunology and immunopathology》2002,86(1-2):23-30
A recombinant bovine interleukin-12 (boIL-12) that contains a histidine hexamer, rboIL-12His, was produced, purified and administered to calves. We first tried the purification of heterodimer IL-12 from a mixture of p40 homodimer, p40 monomer, and p40-p35 heterodimer with a p35 subunit tagged with a histidine hexamar at its C-terminal (p35His). A recombinant baculovirus expressing p35His was generated and used for superinfection with a recombinant baculovirus expressing p40 subunit. The expressed subunits, p40 and p35His, were assembled into a 70kDa heterodimer in insect cells, released into culture medium, and then purified using a nickel chelate column. The purified rboIL-12His was bioactive for induction of IFN-gamma in bovine peripheral blood mononuclear cells (PBMCs) in vitro.The purified rboIL-12His was then administered to calves with inactivated Salmonella Typhimurium (ST). When sera were assayed by ELISA, specific anti-ST IgG1 antibodies were detected in all ST immunized calves, but, specific anti-ST IgG2 antibodies were detected only in calves administered ST along with rboIL-12His, indicating a possible switch to a Th1 response. Administration of commercially available Salmonella vaccine did not elicit IgG2 antibodies in calves. These results suggest that co-administration of IL-12 with inactivated ST cells could induce a Th1-type response in calves. 相似文献
59.
Ayumi SUZUKI Satoko KUSUMOTO Hiromichi HORIE Yuichi TAKIKAWA 《Journal of General Plant Pathology》2002,68(4):398-400
A bacterial leaf spot disease was observed on Hedera helix (English ivy) and H. canariensis (Algerian ivy) in Japan. The causal agent was identified as Xanthomonas campestris pv. hederae (Arnaud 1920) Dye 1978.
Received 13 May 2002/ Accepted in revised form 3 July 2002 相似文献
60.
Fukuda Toshiyuki Sato Yuichi Takiguchi Mako Yamamoto Takahiro Murasawa Hiroyasu Pawlak Akiko Kobayashi Hiroyuki Tamano Haruna Takeda Atsushi 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):279-282
Plant Foods for Human Nutrition - Amyloid β (Aβ) peptides produced from the amyloid precursor protein, a transmembrane protein, are neurotoxic and blocking the neurotoxicity may lead to... 相似文献