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71.
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香蕉试管苗不炼苗移栽技术初探 总被引:1,自引:0,他引:1
20世纪70年代我国台湾省报道了利用香蕉茎尖培养大量种苗的有关研究。大陆80年代初期开始试验该项技术,1985年之后,广东、广西、云南、福建、海南等省(区)组织培养香蕉试管苗都先后进入工厂化生产阶段。与传统吸芽繁殖相比,香蕉组培快速繁殖具有能够迅速推广优良品种;繁殖系数高,周期短,幼苗整齐;收获期一致,有利于集约化经营和商品化生产等诸多优点。 相似文献
73.
奶牛胎衣不下是指奶牛在产后12~24小时内胎衣不能自然完全脱落者,它是奶牛的常见病之一。我国有关资料显示,在一般奶牛场中,此病的发病率为10%~25%,有些地区高达40%以上。据美国1996年有关报道,发病率平均为7.4%。据文献报道:胎衣不下患牛中,有80%以上牛只会继发感染子宫内膜炎,结果导致奶牛产后发情延迟和配种次数增加;40%以上牛只会继发感染乳房炎及产后代谢病,产奶量下降,淘汰率增加。1胎衣不下的原因1.1生理性因素1.1.1胎衣不下具有遗传性,即此胎发生的,下胎发病的概率会增加。1.1.2初产牛难产会使胎衣不下的发病危险程度提高3~4倍。1.1… 相似文献
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V. Villar B. Colao I. Calles‐Venal J. G. Fernndez‐lvarez M. Fernndez‐Caso J. M. Villar 《Anatomia, histologia, embryologia》2005,34(Z1):54-54
Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process. 相似文献
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AIM: To study whether Sca-1+ cells from fetal liver can be induced to differentiate into neuronal cells in vitro. METHODS:Sca-1+cells from 14 5-days-old murine fetal liver were isolated with a magnetic cell sorting kit, and were cultured in Dulbecco s modif ied Eagle s medium(DMEM)/F12 supplemented with 10%fetal bovine serum(FBS), and passaged at a rat io of 1 3 when cells reached more than 80%confluence.The 5 passage cells were induced by 10-3mol/Lβ-mercaptoethanol(β-ME)and 5×10-7 mol/L all-trans-retinoic acid(RA)for 24 hours, and then incubated in serum-free medium for 5 hours to 5 days.The characteristics of treated cel s were assayed by immunocytochemistry staining analysis at 5 hours, or 5 days.RESULTS: Cells treated with β-ME and RA exhibited neuronal phenotype and expressed neuron-specific protein such as neuron-specific nuclear protein (NeuN), neuronfilament-M, and neuron-specific tubulin-1 (TuJ-1) but not tau, MAP-2, or the astrocyte-specific marker glial fibrillary acidic protein (GFAP).CONCLUSION: Sca-1+ cells from fetal liver, of which most are regarded as hematopoietic stem cells, could differentiate into early immature neuronal cells in vitro. These findings suggest that Sca-1+ cells from fetal liver may be an alternative source in cell therapy and gene therapy of neural dysfunction. 相似文献
77.
AIM:To explore transdifferentiation potential of Sca-1+ cells from murine fetal liver. METHODS:2×103 of Sca-1+ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from 60 Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA+/CD45-F4/80- and NueN+/CD45-F4/80-, respectively. CONCLUSION:The evidence is provided for Sca-1+ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells. 相似文献
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苹果粗皮病与锰含量的关系 总被引:14,自引:2,他引:14
对胶东半岛部分发生苹果粗皮病(IBN)的果园的土壤和植株进行了分析测定,结果表明:发生苹果粗皮病的果园土壤有效锰含量较高,而且粗皮病严重果园高于粗皮病较轻果园。发生粗皮病的苹果树花器官中锰含量较高,粗皮病最严重的姜格庄果园苹果树花器官中锰平均含量比观水镇高出139.28mg/kg,比季家埠重病园和轻病园分别高122.34和182.19mg/kg。从4月到10月,苹果粗皮病逐渐加重,叶片中锰含量逐步增高。10月份姜格庄果园最高锰含量达1717.85mg/kg,平均锰含量达1119.69mg/kg,而粗皮病较轻的季家埠果园则仅306.03mg/kg,前者比后者高265.88%。对不同部位锰含量测定表明,锰含量在叶片中最高,其次是韧皮部,木质部最低。表明胶东半岛苹果粗皮病的发生是因为锰过量。可能是土壤锰含量高,pH值低,降雨多,施肥不合理等,造成土壤锰有效性增加.导致树体吸收过量而出现粗皮病害。 相似文献