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81.
82.
Yoshida S Kono K Lowery DM Bartolini S Yaffe MB Ohya Y Pellman D 《Science (New York, N.Y.)》2006,313(5783):108-111
The links between the cell cycle machinery and the cytoskeletal proteins controlling cytokinesis are poorly understood. The small guanine nucleotide triphosphate (GTP)-binding protein RhoA stimulates type II myosin contractility and formin-dependent assembly of the cytokinetic actin contractile ring. We found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange factors. This role of Cdc5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms. 相似文献
83.
Abe Y Hirane A Yoshizawa A Nakajima H Adachi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(10):1089-1091
Serum obtained from a patient histopathologically diagnosed as intestinal spirochetosis was investigated serodiagnostically by agglutination test. B. aalborgi which is a human intestinal spirochete reacted strongly with the human serum, while B. pilosicoli which has potential pathogenicity to humans reacted with the serum, but as strongly and its titer was different than the other three species. On the other hand, intestinal spirochetes (Matsumoto isolates) were isolated from the biopsy samples of the patient. The morphological, biochemical, and genetic characteristics of the isolates were very similar to those of B. aalborgi. Furthermore, the protein profiles of the Matsumoto isolates were also similar to those of B. aalborgi but were different than those of B. pilosicoli and B. hyodysenteriae. The reaction profiles of the Matsumoto isolates in immunoblotting were relatively similar to those of B. aalborgi except for a 74 kDa band but were different from those of B. pilosicoli and B. hyodysenteriae. Therefore, we identified the Matsumoto isolates as B. aalborgi and diagnosed the patient with a B. aalborgi infection. 相似文献
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Successful interspecific hybridization between Cucumis sativus L. and C. hystrix Chakr. 总被引:13,自引:0,他引:13
Jin-feng Chen Jack E. Staub Yosuke Tashiro Shiro Isshiki Sadami Miyazaki 《Euphytica》1997,96(3):413-419
Interspecific F1 hybrids were obtained from a cross between Cucumis sativus L. (2n = 2x = 14) and C. hystrix Chakr. (2n = 2x = 24). Controlled
crossing resulted in fruit containing embryos which were excised and rescued on a Murashige and Skoog solid medium. A total
of 59 vigorous plants were obtained from a fruit containing 159 embryos (37.3% regeneration rate). Hybrid plants were morphologically
uniform. The multiple branching habit, densely brown hairs (especially on corolla and pistil), orange-yellow collora, and
ovate fruit of F1 hybrid plants were similar to that of the C. hystrix paternal parent. While appearance of the first pistillate flower was
more similar to that of C. sativus maternal parent than to C. hystrix, staminate flower appearance was mid-parent in occurence.
The diameter and internode length of stem, shape and size of leaves and flowers were intermediate when compared to the parents.
An elongated green, trilobate style/stigma which was not apparent in either parent was observed in staminate flowers of F1 plants. Similarly, the style/stigma of pistillate flower of F1 plants were longger when compared to their parents. The brown pubescence observed on pistillate flowers of the F1 and C. hystrix was not observed on the C. sativus parent. The somatic chromosome number of F1 plants was 19. Two morphologically distinct groups of chromosomes were observed in the F1 hybrid; 7 relatively large chromosomes characteristic of C. sativus, and 12 smaller chromosomes characteristic of C. hystrix.
Analysis of malate dehydrogenase isozyme banding patterns provided additional comfirmation of hybridity. Reciprocal crossing
of F1 plants to either parent and self-crossing indicated that the hybrids were male and female sterile.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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SUMMARY: In a study of the relationship between structural changes and rheological parameters of raw and cooked abalone meat, abalone Haliotis discus was cooked in boiling water for 3 h, then cut up and separated into cross- and vertical sections. Structural change was observed using a light microscope and scanning electron micrography. The rheological parameters were obtained by stress-relaxation experiments. Microscopic photography revealed that structural change between muscle fibers in both sections of the cooked meat was greater than that in the raw meat. The elastic modulus and rupture strength in both sections of cooked meat showed no difference between the cross- and vertical sections, and were smaller than those for the raw meat. The relaxation time for the cooked meat was also greater than for the raw meat. These results were mainly because of collagen being gelatinized during heating. These changes were also observed by staining collagen and differential scanning calorimetry. 相似文献
89.
Maoka T Akimoto N Tsushima M Komemushi S Mezaki T Iwase F Takahashi Y Sameshima N Mori M Sakagami Y 《Marine drugs》2011,9(8):1419-1427
Carotenoids of the corals Acropora japonica, A. secale, and A. hyacinthus, the tridacnid clam Tridacna squamosa, the crown-of-thorns starfish Acanthaster planci, and the small sea snail Drupella fragum were investigated. The corals and the tridacnid clam are filter feeders and are associated with symbiotic zooxanthellae. Peridinin and pyrrhoxanthin, which originated from symbiotic zooxanthellae, were found to be major carotenoids in corals and the tridacnid clam. The crown-of-thorns starfish and the sea snail D. fragum are carnivorous and mainly feed on corals. Peridinin-3-acyl esters were major carotenoids in the sea snail D. fragum. On the other hand, ketocarotenoids such as 7,8-didehydroastaxanthin and astaxanthin were major carotenoids in the crown-of-thorns starfish. Carotenoids found in these marine animals closely reflected not only their metabolism but also their food chains. 相似文献
90.
Yoshikazu Taketa Akira Inomata Jiro Sonoda Kazuhiro Hayakawa Kyoko Nakano-Ito Etsuko Ohta Yuki Seki Aya Goto Satoru Hosokawa 《Journal of toxicologic pathology》2013,26(4):419-422
Malakoplakia is a rare form of chronic granulomatous inflammation in mammals, and usually affects the urinary tract in humans. In this report, we present a case of granulomatous nephritis consistent with malakoplakia in a 4-year-old male cynomolgus monkey. Gross examination showed that the kidney was markedly enlarged and adhered to the surrounding organs. Histology showed that there was diffuse interstitial infiltration of histiocytes with abundant foamy eosinophilic cytoplasm resembling von Hansemann cells, PAS-positive granular cytoplasm and occasional PAS- and iron-positive intracellular small inclusion bodies. Electron microscopy showed that these histiocytes contained abundant lysosomes and phagolysosomes but no obvious Michaelis-Gutmann bodies. Based on these findings, a diagnosis of granulomatous nephritis consistent with early malakoplakia was made. This is the first report in a monkey of a renal lesion consistent with malakoplakia. 相似文献