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41.
42.
Susan A. Elmore Mark Hoenerhoff Osamu Katsuta Hiroko Kokoshima Robert Maronpot Hiroaki Nagai Hiroshi Satoh Yasuhiro Tanaka Tomoaki Tochitani Seiichiro Tsuchiya Katsuhiko Yoshizawa 《Journal of toxicologic pathology》2013,26(2):231-257
The first joint Japanese Society of Toxicologic Pathology (JSTP) and National Toxicology
Program (NTP) Satellite Symposium, entitled “Pathology Potpourri,” was held on January
29th at Okura Frontier Hotel in Tsukuba, Ibaraki, Japan, in advance of the
JSTP’s 29th Annual Meeting. The goal of this Symposium was to present current
diagnostic pathology or nomenclature issues to the toxicologic pathology community. This
article presents summaries of the speakers’ presentations, including diagnostic or
nomenclature issues that were presented, select images that were used for audience voting
or discussion, and the voting results. Some lesions and topics covered during the
symposium include: treatment-related atypical hepatocellular foci of cellular alteration
in B6C3F1 mice; purulent ventriculoencephalitis in a young BALB/c mouse; a subcutaneous
malignant schwannoma in a RccHan:WIST rat; spontaneous nasal septum
hyalinosis/eosinophilic substance in B6C3F1 mice; a rare pancreatic ductal cell adenoma in
a young Lewis rat; eosinophilic crystalline pneumonia in a transgenic mouse model; hyaline
glomerulopathy in two female ddY mice; treatment-related intrahepatic erythrocytes in
B6C3F1 mice; treatment-related subendothelial hepatocytes in B6C3F1 mice; spontaneous
thyroid follicular cell vacuolar degeneration in a cynomolgus monkey; congenital hepatic
fibrosis in a 1-year-old cat; a spontaneous adenocarcinoma of the middle ear in a young
Crl:CD(SD) rat; and finally a series of cases illustrating some differences between
cholangiofibrosis and cholangiocarcinoma in Sprague Dawley and F344 rats. 相似文献
43.
Yoshie MANABE Makoto TOCHIGI Akiyoshi MORIWAKI Sakae TAKEUCHI Sumio TAKAHASHI 《The Journal of reproduction and development》2013,59(4):398-404
Reproductive functions decline with the onset of diabetes in female mice. Diabetic mice
have smaller uteri with an underdeveloped endometrium, suggesting diminished
estrogen-induced growth. We aimed to clarify the changes in the estrous cycle and in
insulin-like growth factor 1 (IGF1) expression in the uteri of streptozotocin
(STZ)-treated diabetic mice, because IGF1 is one of the main growth factors involved in
estrogen-induced uterine growth. ICR female mice were intraperitoneally administered STZ
(10 mg/100 g BW), and blood glucose levels were determined. Mice with blood glucose levels
> 200 mg/dl were classified as diabetic mice. The onset of diabetes was associated with
acyclic estrous cycles. Diabetes was also induced with STZ in ovariectomized mice. Uterine
Igf1 mRNA levels were reduced in ovariectomized STZ-treated diabetic
mice. Estrogen is known to stimulate Igf1 mRNA expression in the uterus,
but estrogen action was abolished in the uteri of STZ-treated diabetic mice. mRNA
expressions of estrogen receptor α (ERα) and steroid hormone receptor coactivators
(SRC-1/Ncoa1, SRC-2/Ncoa2,
SRC-3/Ncoa3 and CBP/p300/Crebbp) were reduced in the
uteri of ovariectomized STZ-treated diabetic mice. The present study demonstrates that
diabetes induces a decline in female reproductive functions in mice. Igf1
expression in ovariectomized diabetic female mice was decreased, and decreased
responsiveness to estrogen in the uteri of diabetic mice is probably associated with a
reduction in ERα and steroid receptor coactivator mRNA expression. 相似文献
44.
Yamato O Hayashi D Satoh H Shoda T Uchida K Nakayama H Sakai H Masegi T Murai A Iida T Hisada H Hisada A Yamasaki M Maede Y Arai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):813-818
GM2 gangliosidosis variant 0 (human Sandhoff disease) is a lysosomal storage disease caused by simultaneous deficiencies of acid beta-hexosaminidase (Hex) A and Hex B due to an abnormality of beta-subunit, a common component in these enzyme molecules, which is coded by the HEXB gene. In the present study, a retrospective diagnosis was performed in 2 previous suspected cases of feline Sandhoff-like disease using a DNA test to detect the causative mutation identified previously in 4 cats in 2 other families of Japanese domestic cats. Enzymic analysis was also performed using stored leukocytes and plasma collected from the subject families in order to investigate the usefulness of enzymic diagnosis and genotyping of carriers. The DNA test suggested that the 2 cases were homozygous recessive for the mutation. Consequently, 6 cats homozygous for the same mutation have been found in 4 separate locations of Japan, suggesting that this mutant allele may be spread widely in the Japanese domestic cat populations. In enzymic analysis, Hex A and Hex B activities in leukocytes and plasma measured using 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide as a substrate were negligible in affected cats, compared with those in normal and carrier cats. However, there was a wide overlap in enzyme activity between normal and carrier cats. Therefore, it was concluded that enzymic analysis is useful for diagnosis of affected cats, but is not acceptable for genotyping of carriers. 相似文献
45.
Jun Sasaki Megumi Uehara Itaru Sato Hiroshi Satoh Yoshitaka Deguchi Hiroyuki Chida Masahiro Natsuhori Takahisa Murata Kenji Ochiai Kumiko Otani Keiji Okada Nobuhiko Ito 《Animal Science Journal》2019,90(9):1333-1339
To study the effect of ionizing radiation on thyroid glands, 66 Japanese Black cattle residing in the restricted area of the Fukushima Daiichi Nuclear Power Plant in 2013–2017 were pathologically examined. There were no clinical symptoms of thyroid disease in these cattle. Three cases of goiter and seven of atrophy were found in two among the four farms examined. Cases of goiter exhibited normal morphological structure without mass or nodule formation in thyroid glands. Cellular atypia or capsular invasion of the follicular epithelium was absent. The estimated integrated dose of external radiation in goiter cases ranged from maximum 797 mSv to minimum 24 mSv. All lobules in the seven atrophic thyroid glands were affected, but pathological findings, such as inflammatory cell infiltration or stromal fibrosis, were not observed. The estimated integrated dose of external radiation in atrophic thyroids ranged from maximum 589 mSv to minimum 8 mSv. Immunohistochemical analysis of anti‐nitroguanosine and the TUNEL method in goiter and atrophic thyroid glands did not reveal any positive findings. The present study indicates that there was no significant relationship between a radiation effect and pathological findings in any thyroid glands. 相似文献
46.
Itaru Sato Jun Sasaki Hiroshi Satoh Masahiro Natsuhori Takahisa Murata Keiji Okada 《Animal Science Journal》2019,90(8):1090-1095
Blood and urine were compared to clarify which is a better sample for estimating the concentration of radioactive cesium in muscles of live cattle. The most probable concentration of 137Cs in muscles was 21.0 times that in blood, and the error of this estimation was 28%. The concentration of 137Cs in blood was estimated accurately using urine samples. The estimation error did not increase so much (33%), even when the concentration of 137Cs in muscles was estimated using urine samples. On the other hand, the maximum volume of blood that can be collected with one syringe is 50 ml, whereas it is easy to collect 1,000 ml of urine. It took at least 360 min to confirm that a cow meets the legal standard by inspecting 50 ml of blood. However, with 1,000 ml of urine, a 20 min measurement time was sufficient for this purpose. This difference in the required measurement time is critically important for practical use. In addition, urine can be collected by farmers themselves, whereas the blood collection requires a veterinarian. Therefore, urine is a more convenient and practical sample for estimating the contamination level of live cattle with radioactive cesium. 相似文献
47.
Itaru Sato Jun Sasaki Hiroshi Satoh Yoshitaka Deguchi Hiroyuki Chida Masahiro Natsuhori Kumiko Otani Keiji Okada 《Animal Science Journal》2019,90(1):128-134
White blood cells, especially lymphocytes, are susceptible to radiation exposure. In the present study, red blood cell, total white blood cell, and lymphocyte counts were repeatedly measured in cattle living on three farms located in the “difficult‐to‐return zone” of the Fukushima nuclear accident, and compared with two control groups from unaffected areas. Blood cell counts differed significantly between the two control groups, although almost all the values fell within the normal range. The blood cell counts of the cattle in the “difficult‐to‐return zone” varied across sampling times even on the same farms, being sometimes higher or lower than either of the two control groups. However, neither a statistically significant decrease in blood cell counts nor an increase in the rate of cattle with extremely low blood cell counts was observed overall. The estimated cumulative exposure dose for the cattle on the most contaminated farm was within a range of 500–1000 mSv, exceeding the threshold for the lymphopenia. Because of the low dose rate on these farms, potential radiation damages would have been repaired and have not accumulated enough to cause deterministic effects. 相似文献
48.
Malik Ashiq Rabbani Yoshie Murakami Yasuhisa Kuginuki Kenji Takayanagi 《Genetic Resources and Crop Evolution》1998,45(4):307-316
Genetic diversity of 30 radish (Raphanus sativus L.) accessions was investigated at the phenotypic level with morphological characters and at the DNA level using the random amplified polymorphic DNA (RAPD) technique. Thirty-six morpho-physiological traits were recorded from seedling stage to harvest. The 31 primers used generated 202 RAPD bands, of which 158 (78.2%) were polymorphic. Multivariate procedures were used to classify the germplasm on the basis of phenotypic traits and RAPD fragments. Dendrograms were generated for the Euclidean distance from the morphological data and the Nei's genetic distance from the RAPD markers. Phenotypically, all the accessions were classified into four major groups corresponding to the different forms of cultivated radish. The morphological diversity existing within each of these groups suggested that they should be discriminated into the three botanical convarieties, sativusT (large-rooted), caudatus (pod-type) and oleifer (oilseed-type). Clustering of the accessions did not show any pattern of association between the morphological characters and the collection sites. Instead, landrace groups were associated with their morphological similarities and horticultural uses. On the other hand, the intra-specific genetic relationships of several accessions based on RAPD analysis were related primarily to their collection sites rather than to their phenotypic affinities. The level of polymorphism exhibited by the various convarieties could be exploited in genetic mapping populations to tag economically important traits. These genotypes also could serve as a useful germplasm source for root, leaf, pod and seed. This preliminary study of traditional radish landraces from Pakistan provides useful information regarding their horticultural potential. 相似文献
49.
RA Periana DJ Taube S Gamble H Taube T Satoh H Fujii 《Science (New York, N.Y.)》1998,280(5363):560-564
Platinum catalysts are reported for the direct, low-temperature, oxidative conversion of methane to a methanol derivative at greater than 70 percent one-pass yield based on methane. The catalysts are platinum complexes derived from the bidiazine ligand family that are stable, active, and selective for the oxidation of a carbon-hydrogen bond of methane to produce methyl esters. Mechanistic studies show that platinum(II) is the most active oxidation state of platinum for reaction with methane, and are consistent with reaction proceeding through carbon-hydrogen bond activation of methane to generate a platinum-methyl intermediate that is oxidized to generate the methyl ester product. 相似文献
50.
Tatsuro Suzuki Takahiro Noda Toshikazu Morishita Koji Ishiguro Shiori Otsuka Jotaro Aii Ayana Nakano Kenjiro Katsu Katsuhiro Matsui Ryoma Takeshima Noriaki Aoki Takashi Hara Takahiro Hara Rie Satoh 《Plant Breeding》2023,142(5):711-720
The concentration of amylose, which is synthesised using granule-bound starch synthase, affects the physical properties of food. However, no studies have focused on starch properties and physical characteristics of low-amylose buckwheat. Here, we hypothesised that low-amylose buckwheat would be useful to produce new buckwheat products because low-amylose characteristics change the texture of buckwheat food. In this study, we bred relatively low-amylose buckwheat compared to wild type and investigated the causative genes of the traits, starch properties and physical properties of noodles. In the GBSSa mutant, the amylose concentration was lower than that in the wild type. Compared with the wild type, the mutant exhibited the following traits: Amylose concentration decreased by approximately 2%, setback in the Rapid Visco Analyzer decreased by 30 points and the physical characteristics of noodles in the sensory analysis were soft and sticky. These results suggest that this trait may be useful for changing the texture of foods. In addition, the mutant is promising for producing new foods with physical characteristics that are different from those of the wild type. 相似文献