Application of a low-cost database to soil erosion and soil conservation studies in the Awash Basin,Ethiopia

In large-scale land resource planning exercises, Geographical Information Systems (GIS) form a valuable means of storing, retrieving, displaying, and analysing spatially-referenced data, as well as enabling the simulation of the consequences of various alternative development options. However, at this scale only generalized policies for the allocation of finance can be made, and the implementation of those policies will invariably require detailed evaluation at the local level. This suggests that expenditure on the central planning process itself should be limited, with funds reserved for later site studies. The form of GIS adopted centrally should also lend itself to easy use and interpretation by policymakers and planners. These requirements suggest that GIS development using proprietary off-the-shelf database management software may be preferable to high-technology GIS software. This paper outlines the development of such a GIS, based on dBase-III-plus and its internal programming language, in the analysis of problems of soil erosion and soil conservation in the large (110,000 km²) drainage basin of the Awash River in Ethiopia. The methodology employed involves the FAO modification of the Universal Soil Loss Equation, which clearly lacks a secure process basis, but is appropriate for the scale of planning involved and the quality of calibration data available. The GIS is used to map patterns of soil erosion, to identify target areas for conservation on the basis of criteria of sensitivity to land use change, and to assess the costs and benefits of conservation work in those areas. It is suggested that the simple form of GIS involved in this study has much to offer environmental managers in developing countries in the rapid development of plans for soil conservation.     

Effects of season and postharvest storage on the carotenoid content of Solanum phureja potato tubers

The total carotenoid content was determined of tubers from 38 Solanum phureja lines grown in field plots over 3 years. The results indicated a significant difference between years, but the ranking was similar from year to year and the interaction between season and variety was small. Postharvest storage significantly reduced the carotenoid content of the tubers, and reducing the storage temperature further lowered the carotenoid content. Examination of the individual carotenoids revealed that lutein was the most stable and least likely to be reduced, while the levels of the carotenoids derived from beta-carotene were significantly reduced during storage at either temperature. Exposure of the tubers to either mercury or sodium lights resulted in a significant increase in total carotenoid content, concomitant with elevated chlorophyll. Although both types of radiation produced a broadly similar increase in total carotenoid contents, differential effects on the individual carotenoid profile of the light-induced carotenoids were observed.     

A comparison of molecular methods for monitoring soil nematodes and their use as biological indicators

Soil fauna, especially soil nematode communities may be used as indicators for monitoring soil biodiversity and ecological processes. A major drawback facing ecologists is the specialised taxonomic knowledge and labour intensive nature of the work required for traditional morphological identification of soil fauna. We review rapid molecular methods, including: DNA Barcoding or sequencing, PCR-DGGE, PCR-TRFLP and real-time PCR, which could enable an empirical assessment of soil nematode assemblages, in relation to their use as monitoring tools. Based on advantages of: high-throughput; ease of comparison between samples; and rapid data analysis, we argue that PCR-TRFLP is well suited to monitoring purposes.