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41.
Yoshihiko BABA Yasuyuki KAKE Masayuki YOSHIDA Kazumasa UEMATSU 《Fisheries Science》2003,69(3):581-588
ABSTRACT: Fish swim by undulatory contractions of the axial trunk musculature. It has been presumed that a descending signal from the brainstem activates central pattern generators in the spinal cord to make the swimming rhythm. In the carp the electrical or chemical stimulation of a mesencephalic nucleus, the nucleus fasciculi longitudinalis medialis (Nflm), could initiate swimming. However, it has not been established what signals originate from the Nflm in order to make the fish swim. The activity of the Nflm neurons was therefore examined electrophysiologically during fictive swimming in paralyzed carp. Three types of neuronal activities closely related to the swimming rhythms were obtained. The first type was a continuous tonic firing throughout an episode of fictive swimming. Neurons involved in this tonic activity may project to the spinal cord and contribute to the activation of spinal neurons to initiate fish swimming. The second type was accompanied by continual phasic firings occurring in rhythm with the activity of spinal motoneurons. Supposing that the swimming rhythm originates in the spinal cord, the synchronous activity in the brain neurons may suggest that in the nucleus there is a relay neuron conveying the rhythm information from the cord to other neurons there or in the brain. The third type exhibited reduced firing rates during fictive swimming. It is possible that the neurons engaged in this activity may be inhibitory and suppress the activities of other neurons in the nucleus and spinal cord during rest or during decelerating swimming. 相似文献
42.
Genetically modified, herbicide-tolerant (GMHT) Brassica napus plants originating from seed spill have recently been found along roadsides leading from Japanese ports that unload oilseed rape. Such introductions have potential biodiversity effects (as defined by the Cartagena Protocol): these include replacement of native elements in the biota through competitive suppression or hybridization. We conducted surveys in the period 2006–2011 to assess such threats. We examined shifts in the population distribution and occurrence of GMHT plants in 1,029 volunteer introduced assemblages of B. napus, 1,169 of B. juncea, and 184 of B. rapa around 12 ports. GMHT B. napus was found around 10 of 12 ports, but its proportion in the populations varied greatly by year and location. Over the survey period, the distributions of a pure non-GMHT population around Tobata and a pure GMHT population around Hakata increased significantly. However, there was no common trend of population expansion or contraction around the 12 ports. Furthermore, we found no herbicide tolerant B. juncea and B. rapa plants derived from crosses with GMHT B. napus. Therefore, GMHT B. napus is not invading native vegetation surrounding its populations and not likely to cross with congeners in Japanese environment. 相似文献
43.
Hanayama R Tanaka M Miyasaka K Aozasa K Koike M Uchiyama Y Nagata S 《Science (New York, N.Y.)》2004,304(5674):1147-1150
Apoptotic cells expose phosphatidylserine and are swiftly engulfed by macrophages. Milk fat globule epidermal growth factor (EGF) factor 8 (MFG-E8) is a protein that binds to apoptotic cells by recognizing phosphatidylserine and that enhances the engulfment of apoptotic cells by macrophages. We report that tingible body macrophages in the germinal centers of the spleen and lymph nodes strongly express MFG-E8. Many apoptotic lymphocytes were found on the MFG-E8-/- tingible body macrophages, but they were not efficiently engulfed. The MFG-E8-/- mice developed splenomegaly, with the formation of numerous germinal centers, and suffered from glomerulonephritis as a result of autoantibody production. These data demonstrate that MFG-E8 has a critical role in removing apoptotic B cells in the germinal centers and that its failure can lead to autoimmune diseases. 相似文献
44.
Asagiri M Hirai T Kunigami T Kamano S Gober HJ Okamoto K Nishikawa K Latz E Golenbock DT Aoki K Ohya K Imai Y Morishita Y Miyazono K Kato S Saftig P Takayanagi H 《Science (New York, N.Y.)》2008,319(5863):624-627
Cathepsin K was originally identified as an osteoclast-specific lysosomal protease, the inhibitor of which has been considered might have therapeutic potential. We show that inhibition of cathepsin K could potently suppress autoimmune inflammation of the joints as well as osteoclastic bone resorption in autoimmune arthritis. Furthermore, cathepsin K-/- mice were resistant to experimental autoimmune encephalomyelitis. Pharmacological inhibition or targeted disruption of cathepsin K resulted in defective Toll-like receptor 9 signaling in dendritic cells in response to unmethylated CpG DNA, which in turn led to attenuated induction of T helper 17 cells, without affecting the antigen-presenting ability of dendritic cells. These results suggest that cathepsin K plays an important role in the immune system and may serve as a valid therapeutic target in autoimmune diseases. 相似文献
45.
Matsumoto Y Nakatsuji S Kuga K Karaki Y Horie N Shimura Y Sakakibara T Nevidomskyy AH Coleman P 《Science (New York, N.Y.)》2011,331(6015):316-319
Fermi liquid theory, the standard theory of metals, has been challenged by a number of observations of anomalous metallic behavior found in the vicinity of a quantum phase transition. The breakdown of the Fermi liquid is accomplished by fine-tuning the material to a quantum critical point by using a control parameter such as the magnetic field, pressure, or chemical composition. Our high-precision magnetization measurements of the ultrapure f-electron-based superconductor β-YbAlB(4) demonstrate a scaling of its free energy that is indicative of zero-field quantum criticality without tuning in a metal. The breakdown of Fermi liquid behavior takes place in a mixed-valence state, which is in sharp contrast with other known examples of quantum critical f-electron systems that are magnetic Kondo lattice systems with integral valence. 相似文献
46.
47.
With the aim of developing therapeutic agents for strongyloidosis, the disease caused by infection with Strongyloides stercoralis, we established a novel assay technique using S. ratti and S. venezuelensis as models for S. stercoralis. The newly developed assay technique was found to more accurately represent treatment-induced larval paralysis than existing assays. Our method uses paper disks impregnated with the test solution, which even allows materials that are sparingly soluble in water to be tested. An inverted microscope was used to observe the larval states, and these states were recorded using a digital camera. We observed the activities of ivermectin and thiabendazole against larvae and calculated larval motility and velocity. These two factors were then combined to determine the overall viability of larvae at selected concentrations. The activities of the anthelmintics were compared by calculating the concentrations at which 50% viability was demonstrated, or in other words, the concentration at which paralysis was caused in 50% of the individuals (50% paralysis concentration; PC(50)). Evaluations after 24h of exposure yielded the following reproducible PC(50) values for ivermectin and thiabendazole, respectively: S. ratti, 2.4 and 140 microM; and S. venezuelensis, 2.3 and 190 microM. After treatment with ivermectin, there was a tendency for larval motility to be greater than that of the controls at low concentrations, a result that might be associated with its mechanism of action. 相似文献
48.
Park SJ Yoshida N Nishifuji K Sekiguchi M Momoi Y Fukada T Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):63-65
The effects of non-specific immunotherapy with anti-allergic peptides extracted from the urine of human allergic patients (MS-antigen), in two dogs with allergic dermatitis (AD) have been described. Clinically, severe pruritus accompanied by secondary bacterial pyoderma did not respond to conventional therapy with systemic antibiotics. The first clinical change appeared as a significant reduction in pruritus within 3 months, around the time of the 15th injection in both cases. The clinical condition was stabilized after 5 months, allowing the gradual withdrawal of concurrent therapies and an increase of injection intervals. The correlation between the results of intradermal skin tests before and after treatment and the improvement of clinical signs was not obvious. 相似文献
49.
Preimplantation development of embryos in labrador retrievers 总被引:1,自引:0,他引:1
Abe Y Suwa Y Yanagimoto-Ueta Y Suzuki H 《The Journal of reproduction and development》2008,54(2):135-137
Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further develop assisted reproductive techniques in canine species, such as cryopreservation and subsequent embryo transfer. 相似文献
50.
Shimokawa Miyama T Umeki S Baba K Sada K Hiraoka H Endo Y Inokuma H Hisasue M Okuda M Mizuno T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1389-1393
A 4-year-old, intact male Shiba dog was referred to Yamaguchi University Animal Medical Center, Yamaguchi, Japan, for the following complaints: anorexia, lethargy, intermittent fever, gingival bleeding and abdominal purpura. The dog presented with persistent neutropenia. Histopathological examination of a bone marrow sample revealed round to oval structures that resembled Hepatozoon micromerozoites and formed a "wheel-spoke" pattern. Furthermore, mature neutrophils were observed around these structures. PCR and sequencing using bone marrow aspirate confirmed Hepatozoon canis (H. canis) infection. These findings suggest that the neutropenia observed in this case was associated with osteomyelitis due to H. canis infection. This is the first report of neutropenia associated with H. canis infection. H. canis infection can be included in the differential diagnosis in canine cases of neutropenia in areas where the disease is endemic. 相似文献