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91.
Masakazu?HiraideEmail author Yoshifumi?Miyazaki Yasushi?Shibata 《Journal of Wood Science》2004,50(4):358-364
The smell of food is one of the most important factors in assessing its quality. Concerning the smell of dried shiitake mushrooms [Lentinula edodes (Berk.) Pegler], 1,2,3,5,6-pentathiepane, commonly known as lenthionine, has been reported as a key compound. However, other compounds have not been studied sufficiently in connection with smell. From the results of sensory intensity studies and sensory evaluations of dried shiitake mushrooms, a positive significant correlation at 1% risk was observed between sensory intensity and sulfur perception. This showed that the smell of dried shiitake mushrooms was characterized by a sulfurous smell. Also, comparing the sensory intensity with the amounts of volatile components showed positive significant correlations at 1% risk between sensory intensity and three compounds: 1,2,4-trithiolane, 1,2,4,6-tetrathiepane, and lenthionine. Furthermore, significant correlations at 5% risk were obtained between the amounts of these three compounds and sensory intensity by multiple regression analysis. This showed that the smell of dried shiitake mushrooms depended on these compounds. The partial regression coefficient of 1,2,4-trithiolane was larger than those of the others, and so it was proposed that 1,2,4-trithiolane could serve as an indicator to estimate the smell of dried shiitake mushroom. 相似文献
92.
Rahman M. Motiur Yasushi Furukawa Isao Kawata Mohammed Mahabubur Rahman Mahbubul Alam 《Journal of Forest Research》2006,11(2):89-97
Empirical information regarding the role of homestead forests in household economy is essential in understanding the importance
of these resources. Identification of the factors that affect homestead forest production and understanding forest owners'
attitudes toward key forest management issues have great significance in making appropriate policy responses to manage these
resources on a sustainable basis. In Bangladesh, homestead forests are claimed to play an important economic role in rural
livelihoods, but no reliable quantitative information exists. This study was undertaken to investigate the role of homestead
forests in the household economy, examine if forest production and income vary across landholding size classes, to explore
the relationships of homestead forest production with species richness, education level, and household size, and to assess
the attitude of homestead forest owners toward key forest management issues. Results show that homestead forests contribute
15.9% of the household income and generate 51.4 man-days of employment per household per year. Production of homestead forests
significantly varies across landholding size classes. It was observed that forest production depends significantly on species
richness of homestead forests and the education level of forest owners. However, no such relationship was found between forest
production and household size. The study revealed that farming families depend more on forest income than nonfarming families.
Attitudes of forest owners toward key forest management issues differ significantly across landholding size classes. For example,
although the majority of the forest owners prefer fruit species to timber species, the percentage of respondents decreased
as the landholding size class increased, while the opposite trend was observed for timber species. The findings of this study
suggest that diversification of forests and extension of education amongst forest owners would improve forest production.
Furthermore, forest policy should address the concerns of the forest owners in different landholding classes and focus on
their specific requirements to enhance sustainable forest management. 相似文献
93.
Hiromi Yamagawa Satoshi Ito Yasushi Mitsuda Kazuro Fukuzato 《Journal of Forest Research》2006,11(2):99-106
We investigated factors limiting the recovery of natural forest in former large-scale conifer plantations abandoned after
clear-cutting in southwestern Japan. We analyzed forest recovery status (“recovered” sites covered by evergreen broad-leaved
trees, and “unrecovered” sites covered by pioneer community or nonvegetated sites) using aerial photographs and field survey.
We applied logistic regression analyses to evaluate the effects of topography, construction of harvesting roads, distance
from remnant forest, stand condition before clear-cutting, and prior land-use history on forest recovery. Human factors, i.e.,
land use and clear-cutting age, were found to affect to forest recovery more than environmental factors such as topography.
Harvesting roads had the strongest negative impact on forest recovery. Forest recovery after clear-cutting of young sugi plantations
also took longer than after clear-cutting of old sugi plantations or evergreen broad-leaved forests. Furthermore, areas formerly
utilized as meadows recovered less successfully than those that had been managed as coppices. The influences of these factors
were thought to be promoted by the advance reproduction as the regeneration sources for forest recovery. The influence of
stand age before logging suggested an effect of thinning, which might alter the abundance of advanced reproduction in the
understory. However, distance from remnant forest appeared to be less important. An influence of topography was also detected,
but this could be partly explained by the existence of advance reproduction in the understory in certain topographic positions.
Thus, our analysis suggests that regeneration sources originating from advanced reproduction in plantations play a significant
role for the recovery of natural forest after clear-cutting. 相似文献
94.
Enomoto H Li CP Morizane K Ibrahim HR Sugimoto Y Ohki S Ohtomo H Aoki T 《Journal of agricultural and food chemistry》2007,55(6):2392-2398
Beta-lactoglobulin (beta-Lg) was glycated with maltopentaose and subsequently phosphorylated by dry-heating in the presence of pyrophosphate to investigate the structural and functional properties of phosphorylated beta-Lg. The circular dichroism spectra showed that the change of the secondary structure in the beta-Lg molecule by glycation and subsequent phosphorylation was small. The differential scanning calorimetry thermograms of beta-Lg showed that the denaturation temperature of the most stable domain was only slightly affected, whereas the retinol-binding activity of beta-Lg was somewhat reduced by glycation and subsequent phosphorylation. These results indicated that the conformational changes of the beta-Lg molecule by glycation and subsequent phosphorylation were mild. The anti-beta-Lg antibody response was somewhat reduced by glycation, but significant changes were not observed by phosphorylation. Although the stability of beta-Lg against heat-induced insolubility was improved by glycation alone, it was further enhanced by phosphorylation. The calcium phosphate solubilizing ability of beta-Lg was enhanced by phosphorylation following glycation. 相似文献
95.
Yoshida H Mizukoshi T Hirayama K Miyano H 《Journal of agricultural and food chemistry》2007,55(3):551-560
A method for the comprehensive analysis of hydrophilic metabolites, based on a combination of high-performance liquid chromatography and mass spectrometry, is described. We evaluated three types of stationary phases to achieve the separation of highly hydrophilic metabolites. Good chromatographic retention and separation of these metabolites were achieved on a pentafluorophenylpropyl-bonded silica column with gradient elution, using 0.1% aqueous formic acid and acetonitrile as the mobile phase. The optimized conditions allowed the comprehensive determination of the standard 49 kinds of amino acids, 6 kinds of amines, 45 kinds of organic acids, 18 kinds of nucleic bases, 5 kinds of nucleosides, and 14 kinds of nucleotides, and then the linearity, dynamic range, detection limit, and precision of the retention time and the peak area were validated. We applied this method for the targeted analysis of the components in soy sauce. The results from the quantitative determination of amino acids were compared to those obtained with an amino acid analyzer, and the accuracy was in the range between 85 and 119%. The accuracy of other detected components was confirmed to be 105-133% by the recovery rate after the addition of standard compounds. We also applied the method for the nontargeted metabolic profiling of the components in several kinds of soy sauces with the principal component analysis. They were classified by the manufacturing methods, and the components that corresponded to the differences were identified. This method could be useful for the targeted analysis of hydrophilic metabolites as well as their nontargeted metabolic profiling. 相似文献
96.
Kashiwazaki N Okuda Y Seita Y Hisamatsu S Sonoki S Shino M Masaoka T Inomata T 《The Journal of reproduction and development》2006,52(4):511-516
The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rate of forward progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%). In addition, the rates of sperm plasma membrane integrity in lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%, respectively) were significantly (P<0.05) higher than in glycerol (17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and acetamide have higher cryoprotective effects than 1.0 M glycerol for cryopreservation of Japanese white rabbit spermatozoa. 相似文献
97.
Tohno M Shimosato T Moue M Aso H Watanabe K Kawai Y Yamaguchi T Saito T Kitazawa H 《Veterinary research》2006,37(6):791-812
To clarify the crucial role of Toll-like receptor (TLR) 2 and TLR9 in immature gut-associated lymphoid tissues (GALT), we focused on the expression of TLR2 and TLR9 and the immune responses induced by their ligands in the GALT of presuckling newborn swine. Quantitative real-time PCR revealed that TLR2 and TLR9 mRNA were expressed at detectable levels in all tested tissues (heart, thymus, lung, spleen, liver, kidney, skeletal muscle, duodenum, jejunum, ileum, ileal Peyer patches (Pps), and mesenteric lymph nodes (MLN)). In particular, in immature intestinal tissues and GALT, TLR2 and TLR9 mRNA were expressed at higher levels in ileal Pps and MLN than in the duodenum, jejunum, and ileum. We confirmed that the TLR2 and TLR9 proteins were also highly expressed and that their ligands were preferentially recognized by TLR2- or TLR9-expressing cells in the MLN and ileal Pps. Zymosan, CpG2006, and lactic acid bacteria could promote mitogenesis and production of multiple cytokines by the MLN and ileal Pps. In addition, double immunostaining for cytokeratin 18 and either TLR2 or TLR9 revealed that both TLR2 and TLR9 are strongly expressed in the columnar membranous (M) cells. Interestingly, while the apical membrane of the columnar M cells strongly expressed TLR2 protein and preferentially recognized zymosan, both "TLR2 expression on the apical membrane" and "TLR2-mediated zymosan binding" were negligible in neighboring enterocytes. These results indicate that TLR2 and TLR9 allow MLN and ileal Pps to respond to a variety of bacterial components immediately after birth, thereby providing newborns with a host defense system. 相似文献
98.
Resistively detected nuclear spin relaxation measurements in closely separated two-dimensional electron systems reveal strong low-frequency electron-spin fluctuations in the quantum Hall regime. As the temperature is decreased, the spin fluctuations, manifested by a sharp enhancement of the nuclear spin-lattice relaxation rate 1/T1, continue to grow down to the lowest temperature of 66 millikelvin. The observed divergent behavior of 1/T1 signals a gapless spin excitation mode and is a hallmark of canted antiferromagnetic order. Our data demonstrate the realization of a two-dimensional system with planar broken symmetry, in which fluctuations do not freeze out when approaching the zero temperature limit. 相似文献
99.
100.
Aito-Inoue M Ohtsuki K Nakamura Y Park EY Iwai K Morimatsu F Sato K 《Journal of agricultural and food chemistry》2006,54(15):5261-5266
For the isolation and detection of food-derived peptides in blood, an approach based on the derivatization of peptides with phenyl isothiocyanate (PITC) was developed. This approach allows hydrophilic peptides to be resolved and specifically detected by reversed-phase (RP) HPLC. For the rapid capturing and clarification of peptides in human plasma, solid-phase extraction by using a mini spin column (5 mmx5 mm) packed with a strong cation exchanger was used. The clarified peptide fraction was further fractionated by size-exclusion chromatography (SEC). The peptides in the SEC fractions were derivatized with PITC, and the derivatives were resolved by RP-HPLC by using an ammonium acetate buffer or a trifluoroacetic acid system. An automatic peptide sequencer based on Edman degradation with a modified program can directly analyze the resolved derivatives. Some synthetic peptides and food-derived peptides in human plasma were successfully isolated and identified by this approach. 相似文献